An Experimental Study on Antioxidant Enzyme Gene Expression in Trematomus newnesi (Boulenger, 1902) Experimentally Exposed to Perfluoro-Octanoic Acid
Antarctica is the continent with the lowest local human impact; however, it is susceptible to pollution from external sources. Emerging pollutants such as perfluoroalkyl substances pose an increasing threat to this environment and therefore require more in-depth investigations to understand their en...
Published in: | Antioxidants |
---|---|
Main Authors: | , , , , , |
Format: | Text |
Language: | English |
Published: |
Multidisciplinary Digital Publishing Institute
2023
|
Subjects: | |
Online Access: | https://doi.org/10.3390/antiox12020352 |
id |
ftmdpi:oai:mdpi.com:/2076-3921/12/2/352/ |
---|---|
record_format |
openpolar |
spelling |
ftmdpi:oai:mdpi.com:/2076-3921/12/2/352/ 2023-08-20T04:00:56+02:00 An Experimental Study on Antioxidant Enzyme Gene Expression in Trematomus newnesi (Boulenger, 1902) Experimentally Exposed to Perfluoro-Octanoic Acid Sara Pacchini Elisabetta Piva Sophia Schumann Paola Irato Daniela Pellegrino Gianfranco Santovito agris 2023-02-02 application/pdf https://doi.org/10.3390/antiox12020352 EN eng Multidisciplinary Digital Publishing Institute Antioxidant Enzyme Systems https://dx.doi.org/10.3390/antiox12020352 https://creativecommons.org/licenses/by/4.0/ Antioxidants; Volume 12; Issue 2; Pages: 352 Antarctic fish perfluoroalkyl substances PFOA oxidative stress antioxidant defence system Text 2023 ftmdpi https://doi.org/10.3390/antiox12020352 2023-08-01T08:35:57Z Antarctica is the continent with the lowest local human impact; however, it is susceptible to pollution from external sources. Emerging pollutants such as perfluoroalkyl substances pose an increasing threat to this environment and therefore require more in-depth investigations to understand their environmental fate and biological impacts. The present study focuses on expression analysis at the transcriptional level of genes coding for four antioxidant enzymes (sod1, sod2, gpx1, and gpx4) in the liver and kidney of an Antarctic fish species, Trematomus newnesi (Boulenger, 1902). mRNA levels were also assessed in fish exposed to 1.5 μg/L of perfluoro-octanoic acid for 10 days. The kidney showed a higher level of expression than the liver in wildlife specimens. In the liver, the treatment induced an increase in gene expression for all the considered enzymes, whereas in the kidney, it induced a general decrease. The obtained results advance the scientific community’s understanding of how the potential future presence of anthropogenic contaminants in the Southern Ocean can affect the antioxidant system of Antarctic fishes. The presence of pollutants belonging to the perfluoroalkyl substances in the Southern Ocean needs to be continuously monitored in parallel with this type of research. Text Antarc* Antarctic Antarctica Southern Ocean MDPI Open Access Publishing Antarctic Southern Ocean Antioxidants 12 2 352 |
institution |
Open Polar |
collection |
MDPI Open Access Publishing |
op_collection_id |
ftmdpi |
language |
English |
topic |
Antarctic fish perfluoroalkyl substances PFOA oxidative stress antioxidant defence system |
spellingShingle |
Antarctic fish perfluoroalkyl substances PFOA oxidative stress antioxidant defence system Sara Pacchini Elisabetta Piva Sophia Schumann Paola Irato Daniela Pellegrino Gianfranco Santovito An Experimental Study on Antioxidant Enzyme Gene Expression in Trematomus newnesi (Boulenger, 1902) Experimentally Exposed to Perfluoro-Octanoic Acid |
topic_facet |
Antarctic fish perfluoroalkyl substances PFOA oxidative stress antioxidant defence system |
description |
Antarctica is the continent with the lowest local human impact; however, it is susceptible to pollution from external sources. Emerging pollutants such as perfluoroalkyl substances pose an increasing threat to this environment and therefore require more in-depth investigations to understand their environmental fate and biological impacts. The present study focuses on expression analysis at the transcriptional level of genes coding for four antioxidant enzymes (sod1, sod2, gpx1, and gpx4) in the liver and kidney of an Antarctic fish species, Trematomus newnesi (Boulenger, 1902). mRNA levels were also assessed in fish exposed to 1.5 μg/L of perfluoro-octanoic acid for 10 days. The kidney showed a higher level of expression than the liver in wildlife specimens. In the liver, the treatment induced an increase in gene expression for all the considered enzymes, whereas in the kidney, it induced a general decrease. The obtained results advance the scientific community’s understanding of how the potential future presence of anthropogenic contaminants in the Southern Ocean can affect the antioxidant system of Antarctic fishes. The presence of pollutants belonging to the perfluoroalkyl substances in the Southern Ocean needs to be continuously monitored in parallel with this type of research. |
format |
Text |
author |
Sara Pacchini Elisabetta Piva Sophia Schumann Paola Irato Daniela Pellegrino Gianfranco Santovito |
author_facet |
Sara Pacchini Elisabetta Piva Sophia Schumann Paola Irato Daniela Pellegrino Gianfranco Santovito |
author_sort |
Sara Pacchini |
title |
An Experimental Study on Antioxidant Enzyme Gene Expression in Trematomus newnesi (Boulenger, 1902) Experimentally Exposed to Perfluoro-Octanoic Acid |
title_short |
An Experimental Study on Antioxidant Enzyme Gene Expression in Trematomus newnesi (Boulenger, 1902) Experimentally Exposed to Perfluoro-Octanoic Acid |
title_full |
An Experimental Study on Antioxidant Enzyme Gene Expression in Trematomus newnesi (Boulenger, 1902) Experimentally Exposed to Perfluoro-Octanoic Acid |
title_fullStr |
An Experimental Study on Antioxidant Enzyme Gene Expression in Trematomus newnesi (Boulenger, 1902) Experimentally Exposed to Perfluoro-Octanoic Acid |
title_full_unstemmed |
An Experimental Study on Antioxidant Enzyme Gene Expression in Trematomus newnesi (Boulenger, 1902) Experimentally Exposed to Perfluoro-Octanoic Acid |
title_sort |
experimental study on antioxidant enzyme gene expression in trematomus newnesi (boulenger, 1902) experimentally exposed to perfluoro-octanoic acid |
publisher |
Multidisciplinary Digital Publishing Institute |
publishDate |
2023 |
url |
https://doi.org/10.3390/antiox12020352 |
op_coverage |
agris |
geographic |
Antarctic Southern Ocean |
geographic_facet |
Antarctic Southern Ocean |
genre |
Antarc* Antarctic Antarctica Southern Ocean |
genre_facet |
Antarc* Antarctic Antarctica Southern Ocean |
op_source |
Antioxidants; Volume 12; Issue 2; Pages: 352 |
op_relation |
Antioxidant Enzyme Systems https://dx.doi.org/10.3390/antiox12020352 |
op_rights |
https://creativecommons.org/licenses/by/4.0/ |
op_doi |
https://doi.org/10.3390/antiox12020352 |
container_title |
Antioxidants |
container_volume |
12 |
container_issue |
2 |
container_start_page |
352 |
_version_ |
1774721424939810816 |