Effects of Enzymatic- and Ultrasound-Assisted Extraction on Physicochemical and Antioxidant Properties of Collagen Hydrolysate Fractions from Alaska Pollack (Theragra chalcogramma) Skin
Collagen hydrolysate were extracted from Alaska pollock skin using enzymatic (EAE), ultrasound (UAE), or combination of enzymatic and ultrasound (EAE+UAE) treatment. Control (C) was not treated with enzymatic or ultrasound. The extracts from C, EAE, UAE, and EAE+UAE were fractionated with ≤3, 3–10,...
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ftmdpi:oai:mdpi.com:/2076-3921/11/11/2112/ 2023-08-20T03:59:15+02:00 Effects of Enzymatic- and Ultrasound-Assisted Extraction on Physicochemical and Antioxidant Properties of Collagen Hydrolysate Fractions from Alaska Pollack (Theragra chalcogramma) Skin Ju Eun Lee Sang-Kyu Noh Mi Jeong Kim agris 2022-10-26 application/pdf https://doi.org/10.3390/antiox11112112 EN eng Multidisciplinary Digital Publishing Institute Extraction and Industrial Applications of Antioxidants https://dx.doi.org/10.3390/antiox11112112 https://creativecommons.org/licenses/by/4.0/ Antioxidants; Volume 11; Issue 11; Pages: 2112 Alaska pollock skin collagen hydrolysate ultrasound assisted extraction enzyme assisted extraction antioxidant properties microstructure Text 2022 ftmdpi https://doi.org/10.3390/antiox11112112 2023-08-01T07:03:25Z Collagen hydrolysate were extracted from Alaska pollock skin using enzymatic (EAE), ultrasound (UAE), or combination of enzymatic and ultrasound (EAE+UAE) treatment. Control (C) was not treated with enzymatic or ultrasound. The extracts from C, EAE, UAE, and EAE+UAE were fractionated with ≤3, 3–10, 10–30, and ≥30 kDa. Each fraction was evaluated for biological activity and structural properties. All fractions contained high levels of glycine and proline. The ≤3 kDa fraction of control and ultrasound-assisted extracts exhibited the highest antioxidant activity as measured using Trolox equivalent antioxidant capacity, ferric ion reducing antioxidant power, oxygen radical absorbance capacity, and an assay on the inhibition of nitric oxide production by LPS-induced macrophages. The structurally digested collagen was evaluated using FTIR spectra and SDS-PAGE after Alcalase® and ultrasound treatments. The microstructure of collagen hydrolysate was assessed using SEM microscopy; the surface morphology was altered according to fraction size and extraction conditions. Overall, it was determined that enzyme treatment in combination with ultrasound is the most effective procedure for obtaining digested collagen hydrolysate, which could be used to further improve biotechnological processing for the addition of value to marine production chains in the future. Text alaska pollock Theragra chalcogramma Alaska MDPI Open Access Publishing Antioxidants 11 11 2112 |
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Open Polar |
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MDPI Open Access Publishing |
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ftmdpi |
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English |
topic |
Alaska pollock skin collagen hydrolysate ultrasound assisted extraction enzyme assisted extraction antioxidant properties microstructure |
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Alaska pollock skin collagen hydrolysate ultrasound assisted extraction enzyme assisted extraction antioxidant properties microstructure Ju Eun Lee Sang-Kyu Noh Mi Jeong Kim Effects of Enzymatic- and Ultrasound-Assisted Extraction on Physicochemical and Antioxidant Properties of Collagen Hydrolysate Fractions from Alaska Pollack (Theragra chalcogramma) Skin |
topic_facet |
Alaska pollock skin collagen hydrolysate ultrasound assisted extraction enzyme assisted extraction antioxidant properties microstructure |
description |
Collagen hydrolysate were extracted from Alaska pollock skin using enzymatic (EAE), ultrasound (UAE), or combination of enzymatic and ultrasound (EAE+UAE) treatment. Control (C) was not treated with enzymatic or ultrasound. The extracts from C, EAE, UAE, and EAE+UAE were fractionated with ≤3, 3–10, 10–30, and ≥30 kDa. Each fraction was evaluated for biological activity and structural properties. All fractions contained high levels of glycine and proline. The ≤3 kDa fraction of control and ultrasound-assisted extracts exhibited the highest antioxidant activity as measured using Trolox equivalent antioxidant capacity, ferric ion reducing antioxidant power, oxygen radical absorbance capacity, and an assay on the inhibition of nitric oxide production by LPS-induced macrophages. The structurally digested collagen was evaluated using FTIR spectra and SDS-PAGE after Alcalase® and ultrasound treatments. The microstructure of collagen hydrolysate was assessed using SEM microscopy; the surface morphology was altered according to fraction size and extraction conditions. Overall, it was determined that enzyme treatment in combination with ultrasound is the most effective procedure for obtaining digested collagen hydrolysate, which could be used to further improve biotechnological processing for the addition of value to marine production chains in the future. |
format |
Text |
author |
Ju Eun Lee Sang-Kyu Noh Mi Jeong Kim |
author_facet |
Ju Eun Lee Sang-Kyu Noh Mi Jeong Kim |
author_sort |
Ju Eun Lee |
title |
Effects of Enzymatic- and Ultrasound-Assisted Extraction on Physicochemical and Antioxidant Properties of Collagen Hydrolysate Fractions from Alaska Pollack (Theragra chalcogramma) Skin |
title_short |
Effects of Enzymatic- and Ultrasound-Assisted Extraction on Physicochemical and Antioxidant Properties of Collagen Hydrolysate Fractions from Alaska Pollack (Theragra chalcogramma) Skin |
title_full |
Effects of Enzymatic- and Ultrasound-Assisted Extraction on Physicochemical and Antioxidant Properties of Collagen Hydrolysate Fractions from Alaska Pollack (Theragra chalcogramma) Skin |
title_fullStr |
Effects of Enzymatic- and Ultrasound-Assisted Extraction on Physicochemical and Antioxidant Properties of Collagen Hydrolysate Fractions from Alaska Pollack (Theragra chalcogramma) Skin |
title_full_unstemmed |
Effects of Enzymatic- and Ultrasound-Assisted Extraction on Physicochemical and Antioxidant Properties of Collagen Hydrolysate Fractions from Alaska Pollack (Theragra chalcogramma) Skin |
title_sort |
effects of enzymatic- and ultrasound-assisted extraction on physicochemical and antioxidant properties of collagen hydrolysate fractions from alaska pollack (theragra chalcogramma) skin |
publisher |
Multidisciplinary Digital Publishing Institute |
publishDate |
2022 |
url |
https://doi.org/10.3390/antiox11112112 |
op_coverage |
agris |
genre |
alaska pollock Theragra chalcogramma Alaska |
genre_facet |
alaska pollock Theragra chalcogramma Alaska |
op_source |
Antioxidants; Volume 11; Issue 11; Pages: 2112 |
op_relation |
Extraction and Industrial Applications of Antioxidants https://dx.doi.org/10.3390/antiox11112112 |
op_rights |
https://creativecommons.org/licenses/by/4.0/ |
op_doi |
https://doi.org/10.3390/antiox11112112 |
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Antioxidants |
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11 |
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11 |
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2112 |
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