Genetic Mechanism for Antioxidant Activity of Endogenous Enzymes under Salinity and Temperature Stress in Turbot (Scophthalmus maximus)
Three antioxidant properties (corresponding to the enzymes superoxide dismutase (SOD), catalase, and glutathione peroxidase) were measured in the livers of Scophthalmus maximus under different salinities/temperatures (5, 10, 20, 30, and 40‰/17, 20, 23, 25, and 28 °C). Split-plot (SP) analysis, addit...
Published in: | Antioxidants |
---|---|
Main Authors: | , , , , |
Format: | Text |
Language: | English |
Published: |
Multidisciplinary Digital Publishing Institute
2022
|
Subjects: | |
Online Access: | https://doi.org/10.3390/antiox11102062 |
id |
ftmdpi:oai:mdpi.com:/2076-3921/11/10/2062/ |
---|---|
record_format |
openpolar |
spelling |
ftmdpi:oai:mdpi.com:/2076-3921/11/10/2062/ 2023-08-20T04:09:40+02:00 Genetic Mechanism for Antioxidant Activity of Endogenous Enzymes under Salinity and Temperature Stress in Turbot (Scophthalmus maximus) Xinan Wang Aijun Ma Zhihui Huang Zhibin Sun Zhifeng Liu agris 2022-10-19 application/pdf https://doi.org/10.3390/antiox11102062 EN eng Multidisciplinary Digital Publishing Institute https://dx.doi.org/10.3390/antiox11102062 https://creativecommons.org/licenses/by/4.0/ Antioxidants; Volume 11; Issue 10; Pages: 2062 Scophthalmus maximus antioxidant enzymes salinity temperature genotype interactions Text 2022 ftmdpi https://doi.org/10.3390/antiox11102062 2023-08-01T06:57:08Z Three antioxidant properties (corresponding to the enzymes superoxide dismutase (SOD), catalase, and glutathione peroxidase) were measured in the livers of Scophthalmus maximus under different salinities/temperatures (5, 10, 20, 30, and 40‰/17, 20, 23, 25, and 28 °C). Split-plot (SP) analysis, additive main effects, and multiplicative interaction (AMMI) and genotype × environment interaction (GGE) biplots were used to analyze genotype × salinity/temperature interactions for antioxidant properties. The results of the SP analysis show that the activity of the three antioxidant properties was significantly (p < 0.01) affected by salinity/temperature, antioxidant, and salinity/temperature × antioxidant interaction. The results of the AMMI analysis for salinity reveal that the effect of genotype, salinity, and genotype × salinity interaction on antioxidant properties reached a significant level (p < 0.001); 92.1065%, 2.6256%, and 4.4360% of the total sum of squares for antioxidant property activity were attributable to the effects of genotype, salinity, and genotype × salinity interaction, respectively. The results of GGE biplot analysis for salinity reveal differences in the activity ranking of the three antioxidant properties under five salinities; this difference expands with an decrease or increase in salinity from 30‰ (optimum salinity). A salinity of 5‰ had the strongest ability to identify the three antioxidant properties. The five experimental salinities were divided into one region, and SOD activity was the highest in this region. In a comprehensive analysis of stability and activity, SOD had the best activity and stability. The results of AMMI analysis for temperature reveal that genotype, temperature, and genotype × temperature interaction had significant effects on the antioxidant properties (p < 0.001); 82.4720%, 4.0666%, and 12.0968% of the total sum of squares for antioxidant property activity were attributable to the effects of genotype, temperature, and genotype × temperature interaction, ... Text Scophthalmus maximus Turbot MDPI Open Access Publishing Antioxidants 11 10 2062 |
institution |
Open Polar |
collection |
MDPI Open Access Publishing |
op_collection_id |
ftmdpi |
language |
English |
topic |
Scophthalmus maximus antioxidant enzymes salinity temperature genotype interactions |
spellingShingle |
Scophthalmus maximus antioxidant enzymes salinity temperature genotype interactions Xinan Wang Aijun Ma Zhihui Huang Zhibin Sun Zhifeng Liu Genetic Mechanism for Antioxidant Activity of Endogenous Enzymes under Salinity and Temperature Stress in Turbot (Scophthalmus maximus) |
topic_facet |
Scophthalmus maximus antioxidant enzymes salinity temperature genotype interactions |
description |
Three antioxidant properties (corresponding to the enzymes superoxide dismutase (SOD), catalase, and glutathione peroxidase) were measured in the livers of Scophthalmus maximus under different salinities/temperatures (5, 10, 20, 30, and 40‰/17, 20, 23, 25, and 28 °C). Split-plot (SP) analysis, additive main effects, and multiplicative interaction (AMMI) and genotype × environment interaction (GGE) biplots were used to analyze genotype × salinity/temperature interactions for antioxidant properties. The results of the SP analysis show that the activity of the three antioxidant properties was significantly (p < 0.01) affected by salinity/temperature, antioxidant, and salinity/temperature × antioxidant interaction. The results of the AMMI analysis for salinity reveal that the effect of genotype, salinity, and genotype × salinity interaction on antioxidant properties reached a significant level (p < 0.001); 92.1065%, 2.6256%, and 4.4360% of the total sum of squares for antioxidant property activity were attributable to the effects of genotype, salinity, and genotype × salinity interaction, respectively. The results of GGE biplot analysis for salinity reveal differences in the activity ranking of the three antioxidant properties under five salinities; this difference expands with an decrease or increase in salinity from 30‰ (optimum salinity). A salinity of 5‰ had the strongest ability to identify the three antioxidant properties. The five experimental salinities were divided into one region, and SOD activity was the highest in this region. In a comprehensive analysis of stability and activity, SOD had the best activity and stability. The results of AMMI analysis for temperature reveal that genotype, temperature, and genotype × temperature interaction had significant effects on the antioxidant properties (p < 0.001); 82.4720%, 4.0666%, and 12.0968% of the total sum of squares for antioxidant property activity were attributable to the effects of genotype, temperature, and genotype × temperature interaction, ... |
format |
Text |
author |
Xinan Wang Aijun Ma Zhihui Huang Zhibin Sun Zhifeng Liu |
author_facet |
Xinan Wang Aijun Ma Zhihui Huang Zhibin Sun Zhifeng Liu |
author_sort |
Xinan Wang |
title |
Genetic Mechanism for Antioxidant Activity of Endogenous Enzymes under Salinity and Temperature Stress in Turbot (Scophthalmus maximus) |
title_short |
Genetic Mechanism for Antioxidant Activity of Endogenous Enzymes under Salinity and Temperature Stress in Turbot (Scophthalmus maximus) |
title_full |
Genetic Mechanism for Antioxidant Activity of Endogenous Enzymes under Salinity and Temperature Stress in Turbot (Scophthalmus maximus) |
title_fullStr |
Genetic Mechanism for Antioxidant Activity of Endogenous Enzymes under Salinity and Temperature Stress in Turbot (Scophthalmus maximus) |
title_full_unstemmed |
Genetic Mechanism for Antioxidant Activity of Endogenous Enzymes under Salinity and Temperature Stress in Turbot (Scophthalmus maximus) |
title_sort |
genetic mechanism for antioxidant activity of endogenous enzymes under salinity and temperature stress in turbot (scophthalmus maximus) |
publisher |
Multidisciplinary Digital Publishing Institute |
publishDate |
2022 |
url |
https://doi.org/10.3390/antiox11102062 |
op_coverage |
agris |
genre |
Scophthalmus maximus Turbot |
genre_facet |
Scophthalmus maximus Turbot |
op_source |
Antioxidants; Volume 11; Issue 10; Pages: 2062 |
op_relation |
https://dx.doi.org/10.3390/antiox11102062 |
op_rights |
https://creativecommons.org/licenses/by/4.0/ |
op_doi |
https://doi.org/10.3390/antiox11102062 |
container_title |
Antioxidants |
container_volume |
11 |
container_issue |
10 |
container_start_page |
2062 |
_version_ |
1774723265918402560 |