Steroid Hormones Protect against Fluoranthene Ethoxyresorufin-O-Deethylase (EROD) Activity Inhibition

The physiological conditions of an organism may influence its ability to cope with environmental stressors, such as contaminants. Biotransformation and the endocrine system interact with each other to promote animal’s fitness. However, little is known regarding the interaction between hormones and r...

Full description

Bibliographic Details
Published in:Applied Sciences
Main Authors: Carla S. S. Ferreira, Miguel Oliveira, Mário Pacheco, Maria Ana Santos
Format: Text
Language:English
Published: Multidisciplinary Digital Publishing Institute 2022
Subjects:
Online Access:https://doi.org/10.3390/app12063098
id ftmdpi:oai:mdpi.com:/2076-3417/12/6/3098/
record_format openpolar
spelling ftmdpi:oai:mdpi.com:/2076-3417/12/6/3098/ 2023-08-20T03:59:44+02:00 Steroid Hormones Protect against Fluoranthene Ethoxyresorufin-O-Deethylase (EROD) Activity Inhibition Carla S. S. Ferreira Miguel Oliveira Mário Pacheco Maria Ana Santos agris 2022-03-18 application/pdf https://doi.org/10.3390/app12063098 EN eng Multidisciplinary Digital Publishing Institute Environmental Sciences https://dx.doi.org/10.3390/app12063098 https://creativecommons.org/licenses/by/4.0/ Applied Sciences; Volume 12; Issue 6; Pages: 3098 organic contaminants endocrine regulation biotransformation enzymes in vitro effects Text 2022 ftmdpi https://doi.org/10.3390/app12063098 2023-08-01T04:29:39Z The physiological conditions of an organism may influence its ability to cope with environmental stressors, such as contaminants. Biotransformation and the endocrine system interact with each other to promote animal’s fitness. However, little is known regarding the interaction between hormones and response to pollutants such as polycyclic aromatic hydrocarbons (PAHs). In this in vitro study, we aimed to increase the knowledge regarding the effects of steroid hormones on ethoxyresorufin-O-deethylase (EROD) activity inhibited by contaminants. The effects on in vivo induced EROD activity of Anguilla anguilla were assessed by conducting single and combined exposures to fluoranthene (FL) and to physiological levels of two major steroid hormones (cortisol and 17ß-estradiol). Hepatic microsome exposure to the lowest concentrations of FL (0.1 and 0.3 µM), as well as to cortisol and 17ß-estradiol (E2), led to significant EROD activity induction. However, the highest tested concentrations of FL (0.9 and 2.7 µM) significantly inhibited this enzymatic activity. When microsomes were simultaneously exposed to 0.9 µM FL and one of the hormones, both cortisol and E2 were able to decrease the inhibitory effects, with the former completely reverting EROD activity inhibition. These findings support the idea that cortisol and E2 can help prevent the inhibitory effects of PAHs over biotransformation enzymes, highlighting the physiological relevance of these hormones. Text Anguilla anguilla MDPI Open Access Publishing Applied Sciences 12 6 3098
institution Open Polar
collection MDPI Open Access Publishing
op_collection_id ftmdpi
language English
topic organic contaminants
endocrine regulation
biotransformation enzymes
in vitro effects
spellingShingle organic contaminants
endocrine regulation
biotransformation enzymes
in vitro effects
Carla S. S. Ferreira
Miguel Oliveira
Mário Pacheco
Maria Ana Santos
Steroid Hormones Protect against Fluoranthene Ethoxyresorufin-O-Deethylase (EROD) Activity Inhibition
topic_facet organic contaminants
endocrine regulation
biotransformation enzymes
in vitro effects
description The physiological conditions of an organism may influence its ability to cope with environmental stressors, such as contaminants. Biotransformation and the endocrine system interact with each other to promote animal’s fitness. However, little is known regarding the interaction between hormones and response to pollutants such as polycyclic aromatic hydrocarbons (PAHs). In this in vitro study, we aimed to increase the knowledge regarding the effects of steroid hormones on ethoxyresorufin-O-deethylase (EROD) activity inhibited by contaminants. The effects on in vivo induced EROD activity of Anguilla anguilla were assessed by conducting single and combined exposures to fluoranthene (FL) and to physiological levels of two major steroid hormones (cortisol and 17ß-estradiol). Hepatic microsome exposure to the lowest concentrations of FL (0.1 and 0.3 µM), as well as to cortisol and 17ß-estradiol (E2), led to significant EROD activity induction. However, the highest tested concentrations of FL (0.9 and 2.7 µM) significantly inhibited this enzymatic activity. When microsomes were simultaneously exposed to 0.9 µM FL and one of the hormones, both cortisol and E2 were able to decrease the inhibitory effects, with the former completely reverting EROD activity inhibition. These findings support the idea that cortisol and E2 can help prevent the inhibitory effects of PAHs over biotransformation enzymes, highlighting the physiological relevance of these hormones.
format Text
author Carla S. S. Ferreira
Miguel Oliveira
Mário Pacheco
Maria Ana Santos
author_facet Carla S. S. Ferreira
Miguel Oliveira
Mário Pacheco
Maria Ana Santos
author_sort Carla S. S. Ferreira
title Steroid Hormones Protect against Fluoranthene Ethoxyresorufin-O-Deethylase (EROD) Activity Inhibition
title_short Steroid Hormones Protect against Fluoranthene Ethoxyresorufin-O-Deethylase (EROD) Activity Inhibition
title_full Steroid Hormones Protect against Fluoranthene Ethoxyresorufin-O-Deethylase (EROD) Activity Inhibition
title_fullStr Steroid Hormones Protect against Fluoranthene Ethoxyresorufin-O-Deethylase (EROD) Activity Inhibition
title_full_unstemmed Steroid Hormones Protect against Fluoranthene Ethoxyresorufin-O-Deethylase (EROD) Activity Inhibition
title_sort steroid hormones protect against fluoranthene ethoxyresorufin-o-deethylase (erod) activity inhibition
publisher Multidisciplinary Digital Publishing Institute
publishDate 2022
url https://doi.org/10.3390/app12063098
op_coverage agris
genre Anguilla anguilla
genre_facet Anguilla anguilla
op_source Applied Sciences; Volume 12; Issue 6; Pages: 3098
op_relation Environmental Sciences
https://dx.doi.org/10.3390/app12063098
op_rights https://creativecommons.org/licenses/by/4.0/
op_doi https://doi.org/10.3390/app12063098
container_title Applied Sciences
container_volume 12
container_issue 6
container_start_page 3098
_version_ 1774715074334687232