Collagen-Based Bioactive Bromelain Hydrolysate from Salt-Cured Cod Skin

• Published in: Applied Sciences
• Main Authors: Ezequiel R. Coscueta, María Emilia Brassesco, Manuela Pintado
• Format: Text
• Language: English
• Published: Multidisciplinary Digital Publishing Institute 2021
• Subjects: Atlantic cod skin; enzymatic hydrolysis; collagen; fishery by-products; bioactive peptides; bromelain; antioxidant; antihypertensive; atlantic cod
• Online Access: https://doi.org/10.3390/app11188538

Considerable amounts of fish processing by-products are discarded each year. About 30% of this material may be skin and bone. Fish skin has more than 80% of its total protein content as collagen. Furthermore, in recent years, there has been a growing demand for collagen-based peptides due to their beneficial health effects. So, the objective of the present study was to optimise the obtaining bioactive hydrolysates from salt-cured cod skin using the protease Bromelain at 0.5% (w/w) concentration. This study developed a sustainable process that consumes less time and energy and uses an alternative source as raw material. In addition, bromelain allows hydrolysates with important antioxidant (ORAC, 514 μmol Trolox Equivalent/g protein) and antihypertensive activities (inhibition of ACE, IC50 of 166 μg protein/mL) as well as excellent biocompatibility with dermal and subcutaneous cells.