Insights into Early Ontogenesis of Salmo salar: RNA Extraction, Housekeeping Gene Validation and Transcriptional Expression of Important Primordial Germ Cell and Sex-Determination Genes

• Published in: Animals
• Main Authors: Irfan Ahmad Bhat, Milena Malgorzata Dubiel, Eduardo Rodriguez, Zophonías Oddur Jónsson
• Format: Text
• Language: English
• Published: Multidisciplinary Digital Publishing Institute 2023
• Subjects: PGCs; gonads; sex determination; housekeeping genes; S. salar; sterility; Vasa; Salmo salar
• Online Access: https://doi.org/10.3390/ani13061094

The challenge in extracting high-quality RNA impedes the investigation of the transcriptome of developing salmonid embryos. Furthermore, the mRNA expression pattern of important PGC and SD genes during the initial embryonic development of Salmo salar is yet to be studied. So, in the present study, w...