Physicochemical and Biochemical Properties of Trypsin-like Enzyme from Two Sturgeon Species
This work aimed to determine the physicochemical and biochemical properties of trypsin from beluga Huso huso and sevruga Acipenser stellatus, two highly valuable sturgeon species. According to the results obtained from the methods of casein-zymogram and inhibitory activity staining, the molecular we...
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ftmdpi:oai:mdpi.com:/2076-2615/13/5/853/ 2023-08-20T04:05:33+02:00 Physicochemical and Biochemical Properties of Trypsin-like Enzyme from Two Sturgeon Species Abbas Zamani Maryam Khajavi Abdolmohammad Abedian Kenari Masoumeh Haghbin Nazarpak Atefeh Solouk Mina Esmaeili Enric Gisbert agris 2023-02-26 application/pdf https://doi.org/10.3390/ani13050853 EN eng Multidisciplinary Digital Publishing Institute Aquatic Animals https://dx.doi.org/10.3390/ani13050853 https://creativecommons.org/licenses/by/4.0/ Animals; Volume 13; Issue 5; Pages: 853 beluga physicochemical properties sevruga trypsin digestive physiology Text 2023 ftmdpi https://doi.org/10.3390/ani13050853 2023-08-01T09:00:13Z This work aimed to determine the physicochemical and biochemical properties of trypsin from beluga Huso huso and sevruga Acipenser stellatus, two highly valuable sturgeon species. According to the results obtained from the methods of casein-zymogram and inhibitory activity staining, the molecular weight of trypsin for sevruga and beluga was 27.5 and 29.5 kDa, respectively. Optimum pH and temperature values for both trypsins were recorded at 8.5 and 55 °C by BAPNA (a specific substrate), respectively. The stability of both trypsins was well-preserved at pH values from 6.0 to 11.0 and temperatures up to 50 °C. TLCK and SBTI, two specific trypsin inhibitors, showed a significant inhibitory effect on the enzymatic activity of both trypsins (p < 0.05). The enzyme activity was significantly increased in the presence of Ca+2 and surfactants and decreased by oxidizing agents, Cu+2, Zn+2, and Co+2 (p < 0.05). However, univalent ions Na+ and K+ did not show any significant effect on the activity of both trypsins (p > 0.05). The results of our study show that the properties of trypsin from beluga and sevruga are in agreement with data reported in bony fish and can contribute to the clear understanding of trypsin activity in these primitive species. Text Beluga Beluga* MDPI Open Access Publishing Animals 13 5 853 |
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beluga physicochemical properties sevruga trypsin digestive physiology |
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beluga physicochemical properties sevruga trypsin digestive physiology Abbas Zamani Maryam Khajavi Abdolmohammad Abedian Kenari Masoumeh Haghbin Nazarpak Atefeh Solouk Mina Esmaeili Enric Gisbert Physicochemical and Biochemical Properties of Trypsin-like Enzyme from Two Sturgeon Species |
topic_facet |
beluga physicochemical properties sevruga trypsin digestive physiology |
description |
This work aimed to determine the physicochemical and biochemical properties of trypsin from beluga Huso huso and sevruga Acipenser stellatus, two highly valuable sturgeon species. According to the results obtained from the methods of casein-zymogram and inhibitory activity staining, the molecular weight of trypsin for sevruga and beluga was 27.5 and 29.5 kDa, respectively. Optimum pH and temperature values for both trypsins were recorded at 8.5 and 55 °C by BAPNA (a specific substrate), respectively. The stability of both trypsins was well-preserved at pH values from 6.0 to 11.0 and temperatures up to 50 °C. TLCK and SBTI, two specific trypsin inhibitors, showed a significant inhibitory effect on the enzymatic activity of both trypsins (p < 0.05). The enzyme activity was significantly increased in the presence of Ca+2 and surfactants and decreased by oxidizing agents, Cu+2, Zn+2, and Co+2 (p < 0.05). However, univalent ions Na+ and K+ did not show any significant effect on the activity of both trypsins (p > 0.05). The results of our study show that the properties of trypsin from beluga and sevruga are in agreement with data reported in bony fish and can contribute to the clear understanding of trypsin activity in these primitive species. |
format |
Text |
author |
Abbas Zamani Maryam Khajavi Abdolmohammad Abedian Kenari Masoumeh Haghbin Nazarpak Atefeh Solouk Mina Esmaeili Enric Gisbert |
author_facet |
Abbas Zamani Maryam Khajavi Abdolmohammad Abedian Kenari Masoumeh Haghbin Nazarpak Atefeh Solouk Mina Esmaeili Enric Gisbert |
author_sort |
Abbas Zamani |
title |
Physicochemical and Biochemical Properties of Trypsin-like Enzyme from Two Sturgeon Species |
title_short |
Physicochemical and Biochemical Properties of Trypsin-like Enzyme from Two Sturgeon Species |
title_full |
Physicochemical and Biochemical Properties of Trypsin-like Enzyme from Two Sturgeon Species |
title_fullStr |
Physicochemical and Biochemical Properties of Trypsin-like Enzyme from Two Sturgeon Species |
title_full_unstemmed |
Physicochemical and Biochemical Properties of Trypsin-like Enzyme from Two Sturgeon Species |
title_sort |
physicochemical and biochemical properties of trypsin-like enzyme from two sturgeon species |
publisher |
Multidisciplinary Digital Publishing Institute |
publishDate |
2023 |
url |
https://doi.org/10.3390/ani13050853 |
op_coverage |
agris |
genre |
Beluga Beluga* |
genre_facet |
Beluga Beluga* |
op_source |
Animals; Volume 13; Issue 5; Pages: 853 |
op_relation |
Aquatic Animals https://dx.doi.org/10.3390/ani13050853 |
op_rights |
https://creativecommons.org/licenses/by/4.0/ |
op_doi |
https://doi.org/10.3390/ani13050853 |
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Animals |
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13 |
container_issue |
5 |
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853 |
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1774716111657369600 |