Computed Tomography as a Method for Age Determination of Carnivora and Odontocetes with Validation from Individuals with Known Age
Traditional methods for age determination of wildlife include either slicing thin sections off or grinding a tooth, both of which are laborious and invasive. Especially when it comes to ancient and valuable museum samples of rare or extinct species, non-invasive methods are preferable. In this study...
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2023
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ftmdpi:oai:mdpi.com:/2076-2615/13/11/1783/ 2023-08-20T04:09:17+02:00 Computed Tomography as a Method for Age Determination of Carnivora and Odontocetes with Validation from Individuals with Known Age Sina Baier-Stegmaier Carsten Gundlach Mariann Chriél Mette Sif Hansen Christina Vedel-Smith Charlotte Vikkelsø Hansen Daniel Klingberg Johansson Louise Birgitte Henriksen Magnus Wahlberg Charlotte Bie Thøstesen Aage Kristian Olsen Alstrup Kristian Murphy Gregersen Cino Pertoldi Sussie Pagh agris 2023-05-27 application/pdf https://doi.org/10.3390/ani13111783 EN eng Multidisciplinary Digital Publishing Institute https://dx.doi.org/10.3390/ani13111783 https://creativecommons.org/licenses/by/4.0/ Animals; Volume 13; Issue 11; Pages: 1783 museum collections X-ray computed tomography micro-CT µ-CT toothed whale age carnivore age red fox Vulpes vulpes American mink Neogale vison harbor porpoise Phocoena phocoena Text 2023 ftmdpi https://doi.org/10.3390/ani13111783 2023-08-01T10:14:51Z Traditional methods for age determination of wildlife include either slicing thin sections off or grinding a tooth, both of which are laborious and invasive. Especially when it comes to ancient and valuable museum samples of rare or extinct species, non-invasive methods are preferable. In this study, X-ray micro-computed tomography (µ-CT) was verified as an alternative non-invasive method for age determination of three species within the order of Carnivora and suborders Odontoceti. Teeth from 13 red foxes (Vulpes vulpes), 2 American mink (Neogale vison), and 2 harbor porpoises (Phocoena phocoena) of known age were studied using µ-CT. The number of visible dental growth layers in the µ-CT were highly correlated with true age for all three species (R2 = 96%, p < 0.001). In addition, the Bland–Altman plot showed high agreement between the age of individuals and visible dental layers represented in 2D slices of the 3D µ-CT images. The true age of individuals was on average 0.3 (±0.6 SD) years higher than the age interpreted by the µ-CT image, and there was a 95% agreement between the true age and the age interpreted from visible dental layers in the µ-CT. Text Phocoena phocoena toothed whale MDPI Open Access Publishing Animals 13 11 1783 |
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Open Polar |
collection |
MDPI Open Access Publishing |
op_collection_id |
ftmdpi |
language |
English |
topic |
museum collections X-ray computed tomography micro-CT µ-CT toothed whale age carnivore age red fox Vulpes vulpes American mink Neogale vison harbor porpoise Phocoena phocoena |
spellingShingle |
museum collections X-ray computed tomography micro-CT µ-CT toothed whale age carnivore age red fox Vulpes vulpes American mink Neogale vison harbor porpoise Phocoena phocoena Sina Baier-Stegmaier Carsten Gundlach Mariann Chriél Mette Sif Hansen Christina Vedel-Smith Charlotte Vikkelsø Hansen Daniel Klingberg Johansson Louise Birgitte Henriksen Magnus Wahlberg Charlotte Bie Thøstesen Aage Kristian Olsen Alstrup Kristian Murphy Gregersen Cino Pertoldi Sussie Pagh Computed Tomography as a Method for Age Determination of Carnivora and Odontocetes with Validation from Individuals with Known Age |
topic_facet |
museum collections X-ray computed tomography micro-CT µ-CT toothed whale age carnivore age red fox Vulpes vulpes American mink Neogale vison harbor porpoise Phocoena phocoena |
description |
Traditional methods for age determination of wildlife include either slicing thin sections off or grinding a tooth, both of which are laborious and invasive. Especially when it comes to ancient and valuable museum samples of rare or extinct species, non-invasive methods are preferable. In this study, X-ray micro-computed tomography (µ-CT) was verified as an alternative non-invasive method for age determination of three species within the order of Carnivora and suborders Odontoceti. Teeth from 13 red foxes (Vulpes vulpes), 2 American mink (Neogale vison), and 2 harbor porpoises (Phocoena phocoena) of known age were studied using µ-CT. The number of visible dental growth layers in the µ-CT were highly correlated with true age for all three species (R2 = 96%, p < 0.001). In addition, the Bland–Altman plot showed high agreement between the age of individuals and visible dental layers represented in 2D slices of the 3D µ-CT images. The true age of individuals was on average 0.3 (±0.6 SD) years higher than the age interpreted by the µ-CT image, and there was a 95% agreement between the true age and the age interpreted from visible dental layers in the µ-CT. |
format |
Text |
author |
Sina Baier-Stegmaier Carsten Gundlach Mariann Chriél Mette Sif Hansen Christina Vedel-Smith Charlotte Vikkelsø Hansen Daniel Klingberg Johansson Louise Birgitte Henriksen Magnus Wahlberg Charlotte Bie Thøstesen Aage Kristian Olsen Alstrup Kristian Murphy Gregersen Cino Pertoldi Sussie Pagh |
author_facet |
Sina Baier-Stegmaier Carsten Gundlach Mariann Chriél Mette Sif Hansen Christina Vedel-Smith Charlotte Vikkelsø Hansen Daniel Klingberg Johansson Louise Birgitte Henriksen Magnus Wahlberg Charlotte Bie Thøstesen Aage Kristian Olsen Alstrup Kristian Murphy Gregersen Cino Pertoldi Sussie Pagh |
author_sort |
Sina Baier-Stegmaier |
title |
Computed Tomography as a Method for Age Determination of Carnivora and Odontocetes with Validation from Individuals with Known Age |
title_short |
Computed Tomography as a Method for Age Determination of Carnivora and Odontocetes with Validation from Individuals with Known Age |
title_full |
Computed Tomography as a Method for Age Determination of Carnivora and Odontocetes with Validation from Individuals with Known Age |
title_fullStr |
Computed Tomography as a Method for Age Determination of Carnivora and Odontocetes with Validation from Individuals with Known Age |
title_full_unstemmed |
Computed Tomography as a Method for Age Determination of Carnivora and Odontocetes with Validation from Individuals with Known Age |
title_sort |
computed tomography as a method for age determination of carnivora and odontocetes with validation from individuals with known age |
publisher |
Multidisciplinary Digital Publishing Institute |
publishDate |
2023 |
url |
https://doi.org/10.3390/ani13111783 |
op_coverage |
agris |
genre |
Phocoena phocoena toothed whale |
genre_facet |
Phocoena phocoena toothed whale |
op_source |
Animals; Volume 13; Issue 11; Pages: 1783 |
op_relation |
https://dx.doi.org/10.3390/ani13111783 |
op_rights |
https://creativecommons.org/licenses/by/4.0/ |
op_doi |
https://doi.org/10.3390/ani13111783 |
container_title |
Animals |
container_volume |
13 |
container_issue |
11 |
container_start_page |
1783 |
_version_ |
1774722112154501120 |