Case Studies in Polar Bear (Ursus maritimus) Sperm Collection and Cryopreservation Techniques
Assisted reproductive technologies can aid conservation efforts via support of ex situ population management and preservation of genetic material. Data from 38 sperm collection attempts from 17 polar bears (1–5 procedures/bear) were evaluated. Sample collections were attempted via electroejaculation...
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2022
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ftmdpi:oai:mdpi.com:/2076-2615/12/4/430/ 2023-08-20T04:10:17+02:00 Case Studies in Polar Bear (Ursus maritimus) Sperm Collection and Cryopreservation Techniques Jessye Wojtusik Terri L. Roth Erin Curry agris 2022-02-11 application/pdf https://doi.org/10.3390/ani12040430 EN eng Multidisciplinary Digital Publishing Institute Wildlife https://dx.doi.org/10.3390/ani12040430 https://creativecommons.org/licenses/by/4.0/ Animals; Volume 12; Issue 4; Pages: 430 electroejaculation endangered species gamete rescue polar bear sperm cryopreservation sperm rescue testicular tumor urethral catheterization Text 2022 ftmdpi https://doi.org/10.3390/ani12040430 2023-08-01T04:07:45Z Assisted reproductive technologies can aid conservation efforts via support of ex situ population management and preservation of genetic material. Data from 38 sperm collection attempts from 17 polar bears (1–5 procedures/bear) were evaluated. Sample collections were attempted via electroejaculation (EEJ; n = 6), urethral catheterization (UC; n = 25), or sperm rescue (SR; n = 7) during the breeding season (Jan. 1-May 21; n = 27) and nonbreeding season (May 22-Dec. 31; n = 11). Sperm retrieval was successful in 1 EEJ (16.7%), 18 UC (72.0%) and 4 SR (57.1%) collections. Initial sperm motility and viability were 50.0% and 77.0% for EEJ, 64.3 ± 7.4% and 80.9 ± 3.8% for UC, and 56.7 ± 8.8% and 80.5 ± 0.5% for SR. UC and SR were more likely to be successful during the breeding season (84.2–100%) than the nonbreeding season (25.0–33.3%). Testicular tumors were observed in four males (57%) during SR. In total, 13 samples were cryopreserved (n = 1 EEJ, 9 UC, and 3 SR) with egg-yolk-based equine extender (EQ) or OptiXcell (OP). For both extenders, post-thaw motility and viability were reduced by 20–60% and 30–65%, respectively. Further efforts to optimize procedures are warranted, but this summary provides data useful for enhancing the success of polar bear sperm collection and cryopreservation. Text Ursus maritimus MDPI Open Access Publishing Animals 12 4 430 |
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Open Polar |
collection |
MDPI Open Access Publishing |
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ftmdpi |
language |
English |
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electroejaculation endangered species gamete rescue polar bear sperm cryopreservation sperm rescue testicular tumor urethral catheterization |
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electroejaculation endangered species gamete rescue polar bear sperm cryopreservation sperm rescue testicular tumor urethral catheterization Jessye Wojtusik Terri L. Roth Erin Curry Case Studies in Polar Bear (Ursus maritimus) Sperm Collection and Cryopreservation Techniques |
topic_facet |
electroejaculation endangered species gamete rescue polar bear sperm cryopreservation sperm rescue testicular tumor urethral catheterization |
description |
Assisted reproductive technologies can aid conservation efforts via support of ex situ population management and preservation of genetic material. Data from 38 sperm collection attempts from 17 polar bears (1–5 procedures/bear) were evaluated. Sample collections were attempted via electroejaculation (EEJ; n = 6), urethral catheterization (UC; n = 25), or sperm rescue (SR; n = 7) during the breeding season (Jan. 1-May 21; n = 27) and nonbreeding season (May 22-Dec. 31; n = 11). Sperm retrieval was successful in 1 EEJ (16.7%), 18 UC (72.0%) and 4 SR (57.1%) collections. Initial sperm motility and viability were 50.0% and 77.0% for EEJ, 64.3 ± 7.4% and 80.9 ± 3.8% for UC, and 56.7 ± 8.8% and 80.5 ± 0.5% for SR. UC and SR were more likely to be successful during the breeding season (84.2–100%) than the nonbreeding season (25.0–33.3%). Testicular tumors were observed in four males (57%) during SR. In total, 13 samples were cryopreserved (n = 1 EEJ, 9 UC, and 3 SR) with egg-yolk-based equine extender (EQ) or OptiXcell (OP). For both extenders, post-thaw motility and viability were reduced by 20–60% and 30–65%, respectively. Further efforts to optimize procedures are warranted, but this summary provides data useful for enhancing the success of polar bear sperm collection and cryopreservation. |
format |
Text |
author |
Jessye Wojtusik Terri L. Roth Erin Curry |
author_facet |
Jessye Wojtusik Terri L. Roth Erin Curry |
author_sort |
Jessye Wojtusik |
title |
Case Studies in Polar Bear (Ursus maritimus) Sperm Collection and Cryopreservation Techniques |
title_short |
Case Studies in Polar Bear (Ursus maritimus) Sperm Collection and Cryopreservation Techniques |
title_full |
Case Studies in Polar Bear (Ursus maritimus) Sperm Collection and Cryopreservation Techniques |
title_fullStr |
Case Studies in Polar Bear (Ursus maritimus) Sperm Collection and Cryopreservation Techniques |
title_full_unstemmed |
Case Studies in Polar Bear (Ursus maritimus) Sperm Collection and Cryopreservation Techniques |
title_sort |
case studies in polar bear (ursus maritimus) sperm collection and cryopreservation techniques |
publisher |
Multidisciplinary Digital Publishing Institute |
publishDate |
2022 |
url |
https://doi.org/10.3390/ani12040430 |
op_coverage |
agris |
genre |
Ursus maritimus |
genre_facet |
Ursus maritimus |
op_source |
Animals; Volume 12; Issue 4; Pages: 430 |
op_relation |
Wildlife https://dx.doi.org/10.3390/ani12040430 |
op_rights |
https://creativecommons.org/licenses/by/4.0/ |
op_doi |
https://doi.org/10.3390/ani12040430 |
container_title |
Animals |
container_volume |
12 |
container_issue |
4 |
container_start_page |
430 |
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1774724375432396800 |