Establishment of the Primary Avian Gonadal Somatic Cell Lines for Cytogenetic Studies

• Published in: Animals
• Main Authors: Inna E. Pristyazhnyuk, Lyubov P. Malinovskaya, Pavel M. Borodin
• Format: Text
• Language: English
• Published: Multidisciplinary Digital Publishing Institute 2022
• Subjects: avian primary cell culture; songbirds; karyotyping; electroporation; Parus; Avian Studies
• Online Access: https://doi.org/10.3390/ani12131724

The last decade was marked by a steep rise in avian studies at genomic and cellular levels. Cell lines are important tools for in vitro studies in cell biology and cytogenetics. We developed a simple method of primary somatic cell culture establishment from the ovaries of the great tits (Parus major...