What Goes Wrong during Early Development of Artificially Reproduced European Eel Anguilla anguilla? Clues from the Larval Transcriptome and Gene Expression Patterns
In eels, large variations in larval mortality exist, which would impede the viable production of juvenile glass eels in captivity. The transcriptome of European eel larvae was investigated to identify physiological pathways and genes that show differential regulation between non-viable vs. viable la...
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ftmdpi:oai:mdpi.com:/2076-2615/11/6/1710/ 2023-08-20T03:59:42+02:00 What Goes Wrong during Early Development of Artificially Reproduced European Eel Anguilla anguilla? Clues from the Larval Transcriptome and Gene Expression Patterns Pauline Jéhannet Arjan P. Palstra Leon T. N. Heinsbroek Leo Kruijt Ron P. Dirks William Swinkels Hans Komen agris 2021-06-08 application/pdf https://doi.org/10.3390/ani11061710 EN eng Multidisciplinary Digital Publishing Institute Aquatic Animals https://dx.doi.org/10.3390/ani11061710 https://creativecommons.org/licenses/by/4.0/ Animals; Volume 11; Issue 6; Pages: 1710 European eel Anguilla anguilla aquaculture immune system osmoregulation morphogenesis RNA-Seq Text 2021 ftmdpi https://doi.org/10.3390/ani11061710 2023-08-01T01:54:18Z In eels, large variations in larval mortality exist, which would impede the viable production of juvenile glass eels in captivity. The transcriptome of European eel larvae was investigated to identify physiological pathways and genes that show differential regulation between non-viable vs. viable larvae. Expression of genes involved in inflammation and host protection was higher, suggesting that non-viable larvae suffered from microbial infection. Expression of genes involved in osmoregulation was also higher, implying that non-viable larvae tried to maintain homeostasis by strong osmoregulatory adaptation. Expression of genes involved in myogenesis, neural, and sensory development was reduced in the non-viable larvae. Expression of the major histocompatibility complex class-I (mhc1) gene, M-protein (myom2), the dopamine 2B receptor (d2br), the melatonin receptor (mtr1), and heat-shock protein beta-1 (hspb1) showed strong differential regulation and was therefore studied in 1, 8, and 15 days post-hatch (dph) larvae by RT-PCR to comprehend the roles of these genes during ontogeny. Expression patterning of these genes indicated the start of active swimming (8 dph) and feed searching behavior (15 dph) and confirmed immunocompetence immediately after hatching. This study revealed useful insights for improving larval survival by microbial control and salinity reduction. Text Anguilla anguilla MDPI Open Access Publishing Animals 11 6 1710 |
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MDPI Open Access Publishing |
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ftmdpi |
language |
English |
topic |
European eel Anguilla anguilla aquaculture immune system osmoregulation morphogenesis RNA-Seq |
spellingShingle |
European eel Anguilla anguilla aquaculture immune system osmoregulation morphogenesis RNA-Seq Pauline Jéhannet Arjan P. Palstra Leon T. N. Heinsbroek Leo Kruijt Ron P. Dirks William Swinkels Hans Komen What Goes Wrong during Early Development of Artificially Reproduced European Eel Anguilla anguilla? Clues from the Larval Transcriptome and Gene Expression Patterns |
topic_facet |
European eel Anguilla anguilla aquaculture immune system osmoregulation morphogenesis RNA-Seq |
description |
In eels, large variations in larval mortality exist, which would impede the viable production of juvenile glass eels in captivity. The transcriptome of European eel larvae was investigated to identify physiological pathways and genes that show differential regulation between non-viable vs. viable larvae. Expression of genes involved in inflammation and host protection was higher, suggesting that non-viable larvae suffered from microbial infection. Expression of genes involved in osmoregulation was also higher, implying that non-viable larvae tried to maintain homeostasis by strong osmoregulatory adaptation. Expression of genes involved in myogenesis, neural, and sensory development was reduced in the non-viable larvae. Expression of the major histocompatibility complex class-I (mhc1) gene, M-protein (myom2), the dopamine 2B receptor (d2br), the melatonin receptor (mtr1), and heat-shock protein beta-1 (hspb1) showed strong differential regulation and was therefore studied in 1, 8, and 15 days post-hatch (dph) larvae by RT-PCR to comprehend the roles of these genes during ontogeny. Expression patterning of these genes indicated the start of active swimming (8 dph) and feed searching behavior (15 dph) and confirmed immunocompetence immediately after hatching. This study revealed useful insights for improving larval survival by microbial control and salinity reduction. |
format |
Text |
author |
Pauline Jéhannet Arjan P. Palstra Leon T. N. Heinsbroek Leo Kruijt Ron P. Dirks William Swinkels Hans Komen |
author_facet |
Pauline Jéhannet Arjan P. Palstra Leon T. N. Heinsbroek Leo Kruijt Ron P. Dirks William Swinkels Hans Komen |
author_sort |
Pauline Jéhannet |
title |
What Goes Wrong during Early Development of Artificially Reproduced European Eel Anguilla anguilla? Clues from the Larval Transcriptome and Gene Expression Patterns |
title_short |
What Goes Wrong during Early Development of Artificially Reproduced European Eel Anguilla anguilla? Clues from the Larval Transcriptome and Gene Expression Patterns |
title_full |
What Goes Wrong during Early Development of Artificially Reproduced European Eel Anguilla anguilla? Clues from the Larval Transcriptome and Gene Expression Patterns |
title_fullStr |
What Goes Wrong during Early Development of Artificially Reproduced European Eel Anguilla anguilla? Clues from the Larval Transcriptome and Gene Expression Patterns |
title_full_unstemmed |
What Goes Wrong during Early Development of Artificially Reproduced European Eel Anguilla anguilla? Clues from the Larval Transcriptome and Gene Expression Patterns |
title_sort |
what goes wrong during early development of artificially reproduced european eel anguilla anguilla? clues from the larval transcriptome and gene expression patterns |
publisher |
Multidisciplinary Digital Publishing Institute |
publishDate |
2021 |
url |
https://doi.org/10.3390/ani11061710 |
op_coverage |
agris |
genre |
Anguilla anguilla |
genre_facet |
Anguilla anguilla |
op_source |
Animals; Volume 11; Issue 6; Pages: 1710 |
op_relation |
Aquatic Animals https://dx.doi.org/10.3390/ani11061710 |
op_rights |
https://creativecommons.org/licenses/by/4.0/ |
op_doi |
https://doi.org/10.3390/ani11061710 |
container_title |
Animals |
container_volume |
11 |
container_issue |
6 |
container_start_page |
1710 |
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1774714670152679424 |