Utero-Placental Immune Milieu during Normal and Aglepristone-Induced Parturition in the Dog
Maternal immunotolerance is required for the maintenance of pregnancy, in sharp contrast with the uterine pro-inflammatory activity observed during parturition in several species. Correspondingly, in the dog, increased immune signaling at term has been suggested, but a deeper understanding of the ut...
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ftmdpi:oai:mdpi.com:/2076-2615/11/12/3598/ 2023-08-20T04:05:48+02:00 Utero-Placental Immune Milieu during Normal and Aglepristone-Induced Parturition in the Dog Miguel Tavares Pereira Renata Nowaczyk Selim Aslan Serhan S. Ay Mariusz P. Kowalewski agris 2021-12-19 application/pdf https://doi.org/10.3390/ani11123598 EN eng Multidisciplinary Digital Publishing Institute Animal Reproduction https://dx.doi.org/10.3390/ani11123598 https://creativecommons.org/licenses/by/4.0/ Animals; Volume 11; Issue 12; Pages: 3598 dog ( Canis lupus familiaris ) immune system utero-placental parturition aglepristone Text 2021 ftmdpi https://doi.org/10.3390/ani11123598 2023-08-01T03:36:25Z Maternal immunotolerance is required for the maintenance of pregnancy, in sharp contrast with the uterine pro-inflammatory activity observed during parturition in several species. Correspondingly, in the dog, increased immune signaling at term has been suggested, but a deeper understanding of the uterine immune milieu is still missing. Thus, the availability of 30 immune-related factors was assessed in utero-placental samples collected during post-implantation (days 18–25 of pregnancy) and mid-gestation (days 35–40) stages, and at the time of prepartum luteolysis. Gene expression and/or protein localization studies were employed. Samples collected from antigestagen (aglepristone)-treated dogs were further analyzed. Progression of pregnancy was associated with the downregulation of IL1β and upregulation of IL10 (p < 0.05) at mid-gestation. When compared with mid-gestation, a higher availability of several factors was observed at term (e.g., CD206, CD4, TLR4). However, in contrast with natural parturition, MHCII, CD25, CCR7, TNFα, IDO1 and AIF1 were upregulated after aglepristone treatment (p < 0.05), but not TNFR1 or CCL13 (p > 0.05). Altogether, these results show an increased immune activity during canine parturition, involving, i.a., M2 macrophages, Treg and Th cells, with strong support for progesterone-mediated immunomodulation. Furthermore, differences between term and induced parturition/abortion could relate to differences in placental maturation towards parturition and/or functional traits of antigestagens. Text Canis lupus MDPI Open Access Publishing Animals 11 12 3598 |
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MDPI Open Access Publishing |
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ftmdpi |
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English |
topic |
dog ( Canis lupus familiaris ) immune system utero-placental parturition aglepristone |
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dog ( Canis lupus familiaris ) immune system utero-placental parturition aglepristone Miguel Tavares Pereira Renata Nowaczyk Selim Aslan Serhan S. Ay Mariusz P. Kowalewski Utero-Placental Immune Milieu during Normal and Aglepristone-Induced Parturition in the Dog |
topic_facet |
dog ( Canis lupus familiaris ) immune system utero-placental parturition aglepristone |
description |
Maternal immunotolerance is required for the maintenance of pregnancy, in sharp contrast with the uterine pro-inflammatory activity observed during parturition in several species. Correspondingly, in the dog, increased immune signaling at term has been suggested, but a deeper understanding of the uterine immune milieu is still missing. Thus, the availability of 30 immune-related factors was assessed in utero-placental samples collected during post-implantation (days 18–25 of pregnancy) and mid-gestation (days 35–40) stages, and at the time of prepartum luteolysis. Gene expression and/or protein localization studies were employed. Samples collected from antigestagen (aglepristone)-treated dogs were further analyzed. Progression of pregnancy was associated with the downregulation of IL1β and upregulation of IL10 (p < 0.05) at mid-gestation. When compared with mid-gestation, a higher availability of several factors was observed at term (e.g., CD206, CD4, TLR4). However, in contrast with natural parturition, MHCII, CD25, CCR7, TNFα, IDO1 and AIF1 were upregulated after aglepristone treatment (p < 0.05), but not TNFR1 or CCL13 (p > 0.05). Altogether, these results show an increased immune activity during canine parturition, involving, i.a., M2 macrophages, Treg and Th cells, with strong support for progesterone-mediated immunomodulation. Furthermore, differences between term and induced parturition/abortion could relate to differences in placental maturation towards parturition and/or functional traits of antigestagens. |
format |
Text |
author |
Miguel Tavares Pereira Renata Nowaczyk Selim Aslan Serhan S. Ay Mariusz P. Kowalewski |
author_facet |
Miguel Tavares Pereira Renata Nowaczyk Selim Aslan Serhan S. Ay Mariusz P. Kowalewski |
author_sort |
Miguel Tavares Pereira |
title |
Utero-Placental Immune Milieu during Normal and Aglepristone-Induced Parturition in the Dog |
title_short |
Utero-Placental Immune Milieu during Normal and Aglepristone-Induced Parturition in the Dog |
title_full |
Utero-Placental Immune Milieu during Normal and Aglepristone-Induced Parturition in the Dog |
title_fullStr |
Utero-Placental Immune Milieu during Normal and Aglepristone-Induced Parturition in the Dog |
title_full_unstemmed |
Utero-Placental Immune Milieu during Normal and Aglepristone-Induced Parturition in the Dog |
title_sort |
utero-placental immune milieu during normal and aglepristone-induced parturition in the dog |
publisher |
Multidisciplinary Digital Publishing Institute |
publishDate |
2021 |
url |
https://doi.org/10.3390/ani11123598 |
op_coverage |
agris |
genre |
Canis lupus |
genre_facet |
Canis lupus |
op_source |
Animals; Volume 11; Issue 12; Pages: 3598 |
op_relation |
Animal Reproduction https://dx.doi.org/10.3390/ani11123598 |
op_rights |
https://creativecommons.org/licenses/by/4.0/ |
op_doi |
https://doi.org/10.3390/ani11123598 |
container_title |
Animals |
container_volume |
11 |
container_issue |
12 |
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3598 |
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