Optimization of Sperm Cryopreservation Protocol for Peregrine Falcon (Falco peregrinus)
Sperm cryopreservation is a complex process that needs to be adapted to wild and domestic avian species to ensure proper efficiency. Because of its accessibility, the peregrine falcon may be used as a good model for studying other raptor species. To find the most optimal cryopreservation protocol fo...
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ftmdpi:oai:mdpi.com:/2076-2615/10/4/691/ 2023-08-20T04:06:22+02:00 Optimization of Sperm Cryopreservation Protocol for Peregrine Falcon (Falco peregrinus) Beatriz Cardoso Irene Sánchez-Ajofrín Cristina Castaño Olga García-Álvarez Milagros Cristina Esteso Alejandro Maroto-Morales María Iniesta-Cuerda José Julián Garde Julián Santiago-Moreno Ana Josefa Soler agris 2020-04-16 application/pdf https://doi.org/10.3390/ani10040691 EN eng Multidisciplinary Digital Publishing Institute Wildlife https://dx.doi.org/10.3390/ani10040691 https://creativecommons.org/licenses/by/4.0/ Animals; Volume 10; Issue 4; Pages: 691 cryopreservation peregrine falcon spermatozoa freezing/thawing cryoprotectant Text 2020 ftmdpi https://doi.org/10.3390/ani10040691 2023-07-31T23:22:46Z Sperm cryopreservation is a complex process that needs to be adapted to wild and domestic avian species to ensure proper efficiency. Because of its accessibility, the peregrine falcon may be used as a good model for studying other raptor species. To find the most optimal cryopreservation protocol for peregrine falcon ejaculates, sperm parameters such as motility, viability, DNA fragmentation, acrosome integrity, and mitochondrial activity were analyzed under different conditions by varying the freezing method (slow freezing in straws vs. ultrarapid freezing in pellets), thawing conditions (37 °C for 30 s vs. 5 °C for 1 min), type of cryoprotectant (DMA vs. DMSO), and concentration of DMSO (4% vs. 8%). Results show that slow cryopreservation in straws yielded greater percentages (p < 0.05) of motile spermatozoa (22.5% ± 4.4% vs. 0.0% ± 4.1%), viable spermatozoa with intact acrosomes (84.6% ± 4.3% vs. 77.4% ± 4.3%), and spermatozoa with active mitochondria (41.0% ± 6.7% vs.12.8% ± 6.7%), compared with those obtained by the ultrarapid freezing in pellets. However, no differences were found between different thawing conditions. Moreover, all sperm motility parameters were greater (p < 0.05) when DMSO was used during freezing compared with DMA, although the use of 3% and 8% DMSO produced similar results. In conclusion, these results represent important progress in the study of falcon semen cryopreservation protocol, highlighting the crucial steps of the process and the most suitable conditions. Text Falco peregrinus peregrine falcon MDPI Open Access Publishing Animals 10 4 691 |
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MDPI Open Access Publishing |
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ftmdpi |
language |
English |
topic |
cryopreservation peregrine falcon spermatozoa freezing/thawing cryoprotectant |
spellingShingle |
cryopreservation peregrine falcon spermatozoa freezing/thawing cryoprotectant Beatriz Cardoso Irene Sánchez-Ajofrín Cristina Castaño Olga García-Álvarez Milagros Cristina Esteso Alejandro Maroto-Morales María Iniesta-Cuerda José Julián Garde Julián Santiago-Moreno Ana Josefa Soler Optimization of Sperm Cryopreservation Protocol for Peregrine Falcon (Falco peregrinus) |
topic_facet |
cryopreservation peregrine falcon spermatozoa freezing/thawing cryoprotectant |
description |
Sperm cryopreservation is a complex process that needs to be adapted to wild and domestic avian species to ensure proper efficiency. Because of its accessibility, the peregrine falcon may be used as a good model for studying other raptor species. To find the most optimal cryopreservation protocol for peregrine falcon ejaculates, sperm parameters such as motility, viability, DNA fragmentation, acrosome integrity, and mitochondrial activity were analyzed under different conditions by varying the freezing method (slow freezing in straws vs. ultrarapid freezing in pellets), thawing conditions (37 °C for 30 s vs. 5 °C for 1 min), type of cryoprotectant (DMA vs. DMSO), and concentration of DMSO (4% vs. 8%). Results show that slow cryopreservation in straws yielded greater percentages (p < 0.05) of motile spermatozoa (22.5% ± 4.4% vs. 0.0% ± 4.1%), viable spermatozoa with intact acrosomes (84.6% ± 4.3% vs. 77.4% ± 4.3%), and spermatozoa with active mitochondria (41.0% ± 6.7% vs.12.8% ± 6.7%), compared with those obtained by the ultrarapid freezing in pellets. However, no differences were found between different thawing conditions. Moreover, all sperm motility parameters were greater (p < 0.05) when DMSO was used during freezing compared with DMA, although the use of 3% and 8% DMSO produced similar results. In conclusion, these results represent important progress in the study of falcon semen cryopreservation protocol, highlighting the crucial steps of the process and the most suitable conditions. |
format |
Text |
author |
Beatriz Cardoso Irene Sánchez-Ajofrín Cristina Castaño Olga García-Álvarez Milagros Cristina Esteso Alejandro Maroto-Morales María Iniesta-Cuerda José Julián Garde Julián Santiago-Moreno Ana Josefa Soler |
author_facet |
Beatriz Cardoso Irene Sánchez-Ajofrín Cristina Castaño Olga García-Álvarez Milagros Cristina Esteso Alejandro Maroto-Morales María Iniesta-Cuerda José Julián Garde Julián Santiago-Moreno Ana Josefa Soler |
author_sort |
Beatriz Cardoso |
title |
Optimization of Sperm Cryopreservation Protocol for Peregrine Falcon (Falco peregrinus) |
title_short |
Optimization of Sperm Cryopreservation Protocol for Peregrine Falcon (Falco peregrinus) |
title_full |
Optimization of Sperm Cryopreservation Protocol for Peregrine Falcon (Falco peregrinus) |
title_fullStr |
Optimization of Sperm Cryopreservation Protocol for Peregrine Falcon (Falco peregrinus) |
title_full_unstemmed |
Optimization of Sperm Cryopreservation Protocol for Peregrine Falcon (Falco peregrinus) |
title_sort |
optimization of sperm cryopreservation protocol for peregrine falcon (falco peregrinus) |
publisher |
Multidisciplinary Digital Publishing Institute |
publishDate |
2020 |
url |
https://doi.org/10.3390/ani10040691 |
op_coverage |
agris |
genre |
Falco peregrinus peregrine falcon |
genre_facet |
Falco peregrinus peregrine falcon |
op_source |
Animals; Volume 10; Issue 4; Pages: 691 |
op_relation |
Wildlife https://dx.doi.org/10.3390/ani10040691 |
op_rights |
https://creativecommons.org/licenses/by/4.0/ |
op_doi |
https://doi.org/10.3390/ani10040691 |
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Animals |
container_volume |
10 |
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4 |
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691 |
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