Identification of Hepatitis E Virus in the Feces of Red Foxes (Vulpes vulpes)
Orthohepeviruses (HEV) can infect a wide range of animals, showing a relatively strict host specificity; however, its zoonotic potential, natural transmission in the wildlife are less known. Several new HEV-like viruses have been identified in various animal species, including carnivores; however, t...
Published in: | Animals |
---|---|
Main Authors: | , , , , , |
Format: | Text |
Language: | English |
Published: |
Multidisciplinary Digital Publishing Institute
2020
|
Subjects: | |
Online Access: | https://doi.org/10.3390/ani10101841 |
id |
ftmdpi:oai:mdpi.com:/2076-2615/10/10/1841/ |
---|---|
record_format |
openpolar |
spelling |
ftmdpi:oai:mdpi.com:/2076-2615/10/10/1841/ 2023-08-20T04:05:59+02:00 Identification of Hepatitis E Virus in the Feces of Red Foxes (Vulpes vulpes) Zsófia Lanszki Kornélia Kurucz Safia Zeghbib Gábor Kemenesi József Lanszki Ferenc Jakab agris 2020-10-10 application/pdf https://doi.org/10.3390/ani10101841 EN eng Multidisciplinary Digital Publishing Institute Wildlife https://dx.doi.org/10.3390/ani10101841 https://creativecommons.org/licenses/by/4.0/ Animals; Volume 10; Issue 10; Pages: 1841 Hepeviridae Orthohepevirus C cvHEV small mammal zoonoses taxonomy diet Text 2020 ftmdpi https://doi.org/10.3390/ani10101841 2023-08-01T00:14:57Z Orthohepeviruses (HEV) can infect a wide range of animals, showing a relatively strict host specificity; however, its zoonotic potential, natural transmission in the wildlife are less known. Several new HEV-like viruses have been identified in various animal species, including carnivores; however, the phylogenetic relationship among these viruses is poorly resolved, since some of them were known as rodent-related so far. The red fox, the most widespread carnivore worldwide, is a known reservoir of several viruses that transmit from wildlife to humans or domestic animals; they might have a defined role in the circulation of rodent-borne HEV. In this study, we performed a HEV survey by heminested RT-PCR (Reverse Transcription PCR) on red fox fecal samples to investigate the presence of HEV in red foxes living in natural conditions, and to explore the origin of the virus via phylogenetic analysis. Out of the 26 investigated samples, HEV RNA was identified in one sample. Following Sanger sequencing, the novel sequence displayed 91% identity on the nucleotide level with recently published European common vole-HEV derived from Microtus arvalis. In contrast, it shared 85% nucleotide similarity with HEV strains described previously in red foxes. Our results strongly support “the dietary-origin” of unclassified HEV-like strains described from predators that usually prey on rodents. Text Common vole Microtus arvalis MDPI Open Access Publishing Animals 10 10 1841 |
institution |
Open Polar |
collection |
MDPI Open Access Publishing |
op_collection_id |
ftmdpi |
language |
English |
topic |
Hepeviridae Orthohepevirus C cvHEV small mammal zoonoses taxonomy diet |
spellingShingle |
Hepeviridae Orthohepevirus C cvHEV small mammal zoonoses taxonomy diet Zsófia Lanszki Kornélia Kurucz Safia Zeghbib Gábor Kemenesi József Lanszki Ferenc Jakab Identification of Hepatitis E Virus in the Feces of Red Foxes (Vulpes vulpes) |
topic_facet |
Hepeviridae Orthohepevirus C cvHEV small mammal zoonoses taxonomy diet |
description |
Orthohepeviruses (HEV) can infect a wide range of animals, showing a relatively strict host specificity; however, its zoonotic potential, natural transmission in the wildlife are less known. Several new HEV-like viruses have been identified in various animal species, including carnivores; however, the phylogenetic relationship among these viruses is poorly resolved, since some of them were known as rodent-related so far. The red fox, the most widespread carnivore worldwide, is a known reservoir of several viruses that transmit from wildlife to humans or domestic animals; they might have a defined role in the circulation of rodent-borne HEV. In this study, we performed a HEV survey by heminested RT-PCR (Reverse Transcription PCR) on red fox fecal samples to investigate the presence of HEV in red foxes living in natural conditions, and to explore the origin of the virus via phylogenetic analysis. Out of the 26 investigated samples, HEV RNA was identified in one sample. Following Sanger sequencing, the novel sequence displayed 91% identity on the nucleotide level with recently published European common vole-HEV derived from Microtus arvalis. In contrast, it shared 85% nucleotide similarity with HEV strains described previously in red foxes. Our results strongly support “the dietary-origin” of unclassified HEV-like strains described from predators that usually prey on rodents. |
format |
Text |
author |
Zsófia Lanszki Kornélia Kurucz Safia Zeghbib Gábor Kemenesi József Lanszki Ferenc Jakab |
author_facet |
Zsófia Lanszki Kornélia Kurucz Safia Zeghbib Gábor Kemenesi József Lanszki Ferenc Jakab |
author_sort |
Zsófia Lanszki |
title |
Identification of Hepatitis E Virus in the Feces of Red Foxes (Vulpes vulpes) |
title_short |
Identification of Hepatitis E Virus in the Feces of Red Foxes (Vulpes vulpes) |
title_full |
Identification of Hepatitis E Virus in the Feces of Red Foxes (Vulpes vulpes) |
title_fullStr |
Identification of Hepatitis E Virus in the Feces of Red Foxes (Vulpes vulpes) |
title_full_unstemmed |
Identification of Hepatitis E Virus in the Feces of Red Foxes (Vulpes vulpes) |
title_sort |
identification of hepatitis e virus in the feces of red foxes (vulpes vulpes) |
publisher |
Multidisciplinary Digital Publishing Institute |
publishDate |
2020 |
url |
https://doi.org/10.3390/ani10101841 |
op_coverage |
agris |
genre |
Common vole Microtus arvalis |
genre_facet |
Common vole Microtus arvalis |
op_source |
Animals; Volume 10; Issue 10; Pages: 1841 |
op_relation |
Wildlife https://dx.doi.org/10.3390/ani10101841 |
op_rights |
https://creativecommons.org/licenses/by/4.0/ |
op_doi |
https://doi.org/10.3390/ani10101841 |
container_title |
Animals |
container_volume |
10 |
container_issue |
10 |
container_start_page |
1841 |
_version_ |
1774716819919077376 |