Dynamic Immune Response to Vibriosis in Pacific Oyster Crassostrea gigas Larvae during the Infection Process as Supported by Accurate Positioning of GFP-Tagged Vibrio Strains
As the immune system is not fully developed during the larval stage, hatchery culture of bivalve larvae is characterized by frequent mass mortality caused by bacterial pathogens, especially Vibrio spp. However, the knowledge is limited to the pathogenesis of vibriosis in oyster larvae, while the imm...
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ftmdpi:oai:mdpi.com:/2076-2607/9/7/1523/ 2023-08-20T04:06:01+02:00 Dynamic Immune Response to Vibriosis in Pacific Oyster Crassostrea gigas Larvae during the Infection Process as Supported by Accurate Positioning of GFP-Tagged Vibrio Strains Dongdong Wang Alfredo Loor Lobke De Bels Gilbert Van Stappen Wim Van den Broeck Nancy Nevejan agris 2021-07-17 application/pdf https://doi.org/10.3390/microorganisms9071523 EN eng Multidisciplinary Digital Publishing Institute https://dx.doi.org/10.3390/microorganisms9071523 https://creativecommons.org/licenses/by/4.0/ Microorganisms; Volume 9; Issue 7; Pages: 1523 Crassostrea gigas larvae vibriosis pathogenesis GFP histopathology immune system Text 2021 ftmdpi https://doi.org/10.3390/microorganisms9071523 2023-08-01T02:12:39Z As the immune system is not fully developed during the larval stage, hatchery culture of bivalve larvae is characterized by frequent mass mortality caused by bacterial pathogens, especially Vibrio spp. However, the knowledge is limited to the pathogenesis of vibriosis in oyster larvae, while the immune response to pathogenic microorganisms in this early life stage is still far from being fully elucidated. In this study, we combined green fluorescent protein (GFP)-tagging, histological and transcriptomic analyses to clarify the pathogenesis of experimental vibriosis and the mechanisms used by the host Pacific oyster Crassostrea gigas larvae to resist infection. The Vibrio strains first colonized the digestive system and rapidly proliferated, while only the transcription level of IκB kinase (IKK) and nuclear factor κB (NF-κB) associated with signaling transduction were up-regulated in oyster at 18 h post challenge (hpc). The mRNA levels for integrin β-1, peroxinectin, and heat shock protein 70 (HSP70), which are associated with phagocytosis, cell adhesion, and cytoprotection, were not upregulated until 30 hpc when the necrosis already happened in the larval digestive system. This suggested that the immunity in the early stages of C. gigas is not strong enough to prevent vibriosis and future research may focus on the strengthening of the gastrointestinal immune ability to defend vibriosis in bivalve larvae. Text Crassostrea gigas Pacific oyster MDPI Open Access Publishing Pacific Microorganisms 9 7 1523 |
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Open Polar |
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MDPI Open Access Publishing |
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ftmdpi |
language |
English |
topic |
Crassostrea gigas larvae vibriosis pathogenesis GFP histopathology immune system |
spellingShingle |
Crassostrea gigas larvae vibriosis pathogenesis GFP histopathology immune system Dongdong Wang Alfredo Loor Lobke De Bels Gilbert Van Stappen Wim Van den Broeck Nancy Nevejan Dynamic Immune Response to Vibriosis in Pacific Oyster Crassostrea gigas Larvae during the Infection Process as Supported by Accurate Positioning of GFP-Tagged Vibrio Strains |
topic_facet |
Crassostrea gigas larvae vibriosis pathogenesis GFP histopathology immune system |
description |
As the immune system is not fully developed during the larval stage, hatchery culture of bivalve larvae is characterized by frequent mass mortality caused by bacterial pathogens, especially Vibrio spp. However, the knowledge is limited to the pathogenesis of vibriosis in oyster larvae, while the immune response to pathogenic microorganisms in this early life stage is still far from being fully elucidated. In this study, we combined green fluorescent protein (GFP)-tagging, histological and transcriptomic analyses to clarify the pathogenesis of experimental vibriosis and the mechanisms used by the host Pacific oyster Crassostrea gigas larvae to resist infection. The Vibrio strains first colonized the digestive system and rapidly proliferated, while only the transcription level of IκB kinase (IKK) and nuclear factor κB (NF-κB) associated with signaling transduction were up-regulated in oyster at 18 h post challenge (hpc). The mRNA levels for integrin β-1, peroxinectin, and heat shock protein 70 (HSP70), which are associated with phagocytosis, cell adhesion, and cytoprotection, were not upregulated until 30 hpc when the necrosis already happened in the larval digestive system. This suggested that the immunity in the early stages of C. gigas is not strong enough to prevent vibriosis and future research may focus on the strengthening of the gastrointestinal immune ability to defend vibriosis in bivalve larvae. |
format |
Text |
author |
Dongdong Wang Alfredo Loor Lobke De Bels Gilbert Van Stappen Wim Van den Broeck Nancy Nevejan |
author_facet |
Dongdong Wang Alfredo Loor Lobke De Bels Gilbert Van Stappen Wim Van den Broeck Nancy Nevejan |
author_sort |
Dongdong Wang |
title |
Dynamic Immune Response to Vibriosis in Pacific Oyster Crassostrea gigas Larvae during the Infection Process as Supported by Accurate Positioning of GFP-Tagged Vibrio Strains |
title_short |
Dynamic Immune Response to Vibriosis in Pacific Oyster Crassostrea gigas Larvae during the Infection Process as Supported by Accurate Positioning of GFP-Tagged Vibrio Strains |
title_full |
Dynamic Immune Response to Vibriosis in Pacific Oyster Crassostrea gigas Larvae during the Infection Process as Supported by Accurate Positioning of GFP-Tagged Vibrio Strains |
title_fullStr |
Dynamic Immune Response to Vibriosis in Pacific Oyster Crassostrea gigas Larvae during the Infection Process as Supported by Accurate Positioning of GFP-Tagged Vibrio Strains |
title_full_unstemmed |
Dynamic Immune Response to Vibriosis in Pacific Oyster Crassostrea gigas Larvae during the Infection Process as Supported by Accurate Positioning of GFP-Tagged Vibrio Strains |
title_sort |
dynamic immune response to vibriosis in pacific oyster crassostrea gigas larvae during the infection process as supported by accurate positioning of gfp-tagged vibrio strains |
publisher |
Multidisciplinary Digital Publishing Institute |
publishDate |
2021 |
url |
https://doi.org/10.3390/microorganisms9071523 |
op_coverage |
agris |
geographic |
Pacific |
geographic_facet |
Pacific |
genre |
Crassostrea gigas Pacific oyster |
genre_facet |
Crassostrea gigas Pacific oyster |
op_source |
Microorganisms; Volume 9; Issue 7; Pages: 1523 |
op_relation |
https://dx.doi.org/10.3390/microorganisms9071523 |
op_rights |
https://creativecommons.org/licenses/by/4.0/ |
op_doi |
https://doi.org/10.3390/microorganisms9071523 |
container_title |
Microorganisms |
container_volume |
9 |
container_issue |
7 |
container_start_page |
1523 |
_version_ |
1774716924914040832 |