Statistical Optimisation of Diesel Biodegradation at Low Temperatures by an Antarctic Marine Bacterial Consortium Isolated from Non-Contaminated Seawater
Hydrocarbon pollution is widespread around the globe and, even in the remoteness of Antarctica, the impacts of hydrocarbons from anthropogenic sources are still apparent. Antarctica’s chronically cold temperatures and other extreme environmental conditions reduce the rates of biological processes, i...
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ftmdpi:oai:mdpi.com:/2076-2607/9/6/1213/ 2023-08-20T04:00:19+02:00 Statistical Optimisation of Diesel Biodegradation at Low Temperatures by an Antarctic Marine Bacterial Consortium Isolated from Non-Contaminated Seawater Nur Nadhirah Zakaria Claudio Gomez-Fuentes Khalilah Abdul Khalil Peter Convey Ahmad Fareez Ahmad Roslee Azham Zulkharnain Suriana Sabri Noor Azmi Shaharuddin Leyla Cárdenas Siti Aqlima Ahmad agris 2021-06-03 application/pdf https://doi.org/10.3390/microorganisms9061213 EN eng Multidisciplinary Digital Publishing Institute Environmental Microbiology https://dx.doi.org/10.3390/microorganisms9061213 https://creativecommons.org/licenses/by/4.0/ Microorganisms; Volume 9; Issue 6; Pages: 1213 Antarctica biodegradation diesel microbial consortium seawater Text 2021 ftmdpi https://doi.org/10.3390/microorganisms9061213 2023-08-01T01:52:40Z Hydrocarbon pollution is widespread around the globe and, even in the remoteness of Antarctica, the impacts of hydrocarbons from anthropogenic sources are still apparent. Antarctica’s chronically cold temperatures and other extreme environmental conditions reduce the rates of biological processes, including the biodegradation of pollutants. However, the native Antarctic microbial diversity provides a reservoir of cold-adapted microorganisms, some of which have the potential for biodegradation. This study evaluated the diesel hydrocarbon-degrading ability of a psychrotolerant marine bacterial consortium obtained from the coast of the north-west Antarctic Peninsula. The consortium’s growth conditions were optimised using one-factor-at-a-time (OFAT) and statistical response surface methodology (RSM), which identified optimal growth conditions of pH 8.0, 10 °C, 25 ppt NaCl and 1.5 g/L NH4NO3. The predicted model was highly significant and confirmed that the parameters’ salinity, temperature, nitrogen concentration and initial diesel concentration significantly influenced diesel biodegradation. Using the optimised values generated by RSM, a mass reduction of 12.23 mg/mL from the initial 30.518 mg/mL (4% (w/v)) concentration of diesel was achieved within a 6 d incubation period. This study provides further evidence for the presence of native hydrocarbon-degrading bacteria in non-contaminated Antarctic seawater. Text Antarc* Antarctic Antarctic Peninsula Antarctica MDPI Open Access Publishing Antarctic Antarctic Peninsula Microorganisms 9 6 1213 |
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ftmdpi |
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English |
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Antarctica biodegradation diesel microbial consortium seawater |
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Antarctica biodegradation diesel microbial consortium seawater Nur Nadhirah Zakaria Claudio Gomez-Fuentes Khalilah Abdul Khalil Peter Convey Ahmad Fareez Ahmad Roslee Azham Zulkharnain Suriana Sabri Noor Azmi Shaharuddin Leyla Cárdenas Siti Aqlima Ahmad Statistical Optimisation of Diesel Biodegradation at Low Temperatures by an Antarctic Marine Bacterial Consortium Isolated from Non-Contaminated Seawater |
topic_facet |
Antarctica biodegradation diesel microbial consortium seawater |
description |
Hydrocarbon pollution is widespread around the globe and, even in the remoteness of Antarctica, the impacts of hydrocarbons from anthropogenic sources are still apparent. Antarctica’s chronically cold temperatures and other extreme environmental conditions reduce the rates of biological processes, including the biodegradation of pollutants. However, the native Antarctic microbial diversity provides a reservoir of cold-adapted microorganisms, some of which have the potential for biodegradation. This study evaluated the diesel hydrocarbon-degrading ability of a psychrotolerant marine bacterial consortium obtained from the coast of the north-west Antarctic Peninsula. The consortium’s growth conditions were optimised using one-factor-at-a-time (OFAT) and statistical response surface methodology (RSM), which identified optimal growth conditions of pH 8.0, 10 °C, 25 ppt NaCl and 1.5 g/L NH4NO3. The predicted model was highly significant and confirmed that the parameters’ salinity, temperature, nitrogen concentration and initial diesel concentration significantly influenced diesel biodegradation. Using the optimised values generated by RSM, a mass reduction of 12.23 mg/mL from the initial 30.518 mg/mL (4% (w/v)) concentration of diesel was achieved within a 6 d incubation period. This study provides further evidence for the presence of native hydrocarbon-degrading bacteria in non-contaminated Antarctic seawater. |
format |
Text |
author |
Nur Nadhirah Zakaria Claudio Gomez-Fuentes Khalilah Abdul Khalil Peter Convey Ahmad Fareez Ahmad Roslee Azham Zulkharnain Suriana Sabri Noor Azmi Shaharuddin Leyla Cárdenas Siti Aqlima Ahmad |
author_facet |
Nur Nadhirah Zakaria Claudio Gomez-Fuentes Khalilah Abdul Khalil Peter Convey Ahmad Fareez Ahmad Roslee Azham Zulkharnain Suriana Sabri Noor Azmi Shaharuddin Leyla Cárdenas Siti Aqlima Ahmad |
author_sort |
Nur Nadhirah Zakaria |
title |
Statistical Optimisation of Diesel Biodegradation at Low Temperatures by an Antarctic Marine Bacterial Consortium Isolated from Non-Contaminated Seawater |
title_short |
Statistical Optimisation of Diesel Biodegradation at Low Temperatures by an Antarctic Marine Bacterial Consortium Isolated from Non-Contaminated Seawater |
title_full |
Statistical Optimisation of Diesel Biodegradation at Low Temperatures by an Antarctic Marine Bacterial Consortium Isolated from Non-Contaminated Seawater |
title_fullStr |
Statistical Optimisation of Diesel Biodegradation at Low Temperatures by an Antarctic Marine Bacterial Consortium Isolated from Non-Contaminated Seawater |
title_full_unstemmed |
Statistical Optimisation of Diesel Biodegradation at Low Temperatures by an Antarctic Marine Bacterial Consortium Isolated from Non-Contaminated Seawater |
title_sort |
statistical optimisation of diesel biodegradation at low temperatures by an antarctic marine bacterial consortium isolated from non-contaminated seawater |
publisher |
Multidisciplinary Digital Publishing Institute |
publishDate |
2021 |
url |
https://doi.org/10.3390/microorganisms9061213 |
op_coverage |
agris |
geographic |
Antarctic Antarctic Peninsula |
geographic_facet |
Antarctic Antarctic Peninsula |
genre |
Antarc* Antarctic Antarctic Peninsula Antarctica |
genre_facet |
Antarc* Antarctic Antarctic Peninsula Antarctica |
op_source |
Microorganisms; Volume 9; Issue 6; Pages: 1213 |
op_relation |
Environmental Microbiology https://dx.doi.org/10.3390/microorganisms9061213 |
op_rights |
https://creativecommons.org/licenses/by/4.0/ |
op_doi |
https://doi.org/10.3390/microorganisms9061213 |
container_title |
Microorganisms |
container_volume |
9 |
container_issue |
6 |
container_start_page |
1213 |
_version_ |
1774717507788079104 |