Investigation of the Initial Host Response of Naïve Atlantic Salmon (Salmo salar) Inoculated with Paramoeba perurans
Amoebic Gill Disease (AGD), caused by the ectoparasite Paramoeba perurans is characterised by hyperplasia of the gill epithelium and lamellar fusion. In this study, the initial host response of naïve Atlantic salmon (Salmo salar) inoculated with P. perurans was investigated. Using gel-free proteomic...
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ftmdpi:oai:mdpi.com:/2076-2607/9/4/746/ 2023-08-20T04:05:16+02:00 Investigation of the Initial Host Response of Naïve Atlantic Salmon (Salmo salar) Inoculated with Paramoeba perurans Michelle McCormack Eugene Dillon Ian O’Connor Eugene MacCarthy agris 2021-04-02 application/pdf https://doi.org/10.3390/microorganisms9040746 EN eng Multidisciplinary Digital Publishing Institute Parasitology https://dx.doi.org/10.3390/microorganisms9040746 https://creativecommons.org/licenses/by/4.0/ Microorganisms; Volume 9; Issue 4; Pages: 746 amoebic gill disease Atlantic salmon gel-free proteomics gill proteome serum proteome Text 2021 ftmdpi https://doi.org/10.3390/microorganisms9040746 2023-08-01T01:25:20Z Amoebic Gill Disease (AGD), caused by the ectoparasite Paramoeba perurans is characterised by hyperplasia of the gill epithelium and lamellar fusion. In this study, the initial host response of naïve Atlantic salmon (Salmo salar) inoculated with P. perurans was investigated. Using gel-free proteomic techniques and mass spectrometry gill and serum samples were analysed at 7 timepoints (2, 3, 4, 7, 9, 11 and 14 days) post-inoculation with P. perurans. Differential expression of immune related proteins was assessed by comparison of protein expression from each time point against naïve controls. Few host immune molecules associated with innate immunity showed increased expression in response to gill colonisation by amoebae. Furthermore, many proteins with roles in immune signalling, phagocytosis and T-cell proliferation were found to be inhibited upon disease progression. Initially, various immune factors demonstrated the anticipated increase in expression in response to infection in the serum while some immune inhibition became apparent at the later stages of disease progression. Taken together, the pro-immune trend observed in serum, the lack of a robust early immune response in the gill and the diversity of those proteins in the gill whose altered expression negatively impact the immune response, support the concept of a pathogen-derived suppression of the host response. Text Atlantic salmon Salmo salar MDPI Open Access Publishing Microorganisms 9 4 746 |
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Open Polar |
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MDPI Open Access Publishing |
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English |
topic |
amoebic gill disease Atlantic salmon gel-free proteomics gill proteome serum proteome |
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amoebic gill disease Atlantic salmon gel-free proteomics gill proteome serum proteome Michelle McCormack Eugene Dillon Ian O’Connor Eugene MacCarthy Investigation of the Initial Host Response of Naïve Atlantic Salmon (Salmo salar) Inoculated with Paramoeba perurans |
topic_facet |
amoebic gill disease Atlantic salmon gel-free proteomics gill proteome serum proteome |
description |
Amoebic Gill Disease (AGD), caused by the ectoparasite Paramoeba perurans is characterised by hyperplasia of the gill epithelium and lamellar fusion. In this study, the initial host response of naïve Atlantic salmon (Salmo salar) inoculated with P. perurans was investigated. Using gel-free proteomic techniques and mass spectrometry gill and serum samples were analysed at 7 timepoints (2, 3, 4, 7, 9, 11 and 14 days) post-inoculation with P. perurans. Differential expression of immune related proteins was assessed by comparison of protein expression from each time point against naïve controls. Few host immune molecules associated with innate immunity showed increased expression in response to gill colonisation by amoebae. Furthermore, many proteins with roles in immune signalling, phagocytosis and T-cell proliferation were found to be inhibited upon disease progression. Initially, various immune factors demonstrated the anticipated increase in expression in response to infection in the serum while some immune inhibition became apparent at the later stages of disease progression. Taken together, the pro-immune trend observed in serum, the lack of a robust early immune response in the gill and the diversity of those proteins in the gill whose altered expression negatively impact the immune response, support the concept of a pathogen-derived suppression of the host response. |
format |
Text |
author |
Michelle McCormack Eugene Dillon Ian O’Connor Eugene MacCarthy |
author_facet |
Michelle McCormack Eugene Dillon Ian O’Connor Eugene MacCarthy |
author_sort |
Michelle McCormack |
title |
Investigation of the Initial Host Response of Naïve Atlantic Salmon (Salmo salar) Inoculated with Paramoeba perurans |
title_short |
Investigation of the Initial Host Response of Naïve Atlantic Salmon (Salmo salar) Inoculated with Paramoeba perurans |
title_full |
Investigation of the Initial Host Response of Naïve Atlantic Salmon (Salmo salar) Inoculated with Paramoeba perurans |
title_fullStr |
Investigation of the Initial Host Response of Naïve Atlantic Salmon (Salmo salar) Inoculated with Paramoeba perurans |
title_full_unstemmed |
Investigation of the Initial Host Response of Naïve Atlantic Salmon (Salmo salar) Inoculated with Paramoeba perurans |
title_sort |
investigation of the initial host response of naïve atlantic salmon (salmo salar) inoculated with paramoeba perurans |
publisher |
Multidisciplinary Digital Publishing Institute |
publishDate |
2021 |
url |
https://doi.org/10.3390/microorganisms9040746 |
op_coverage |
agris |
genre |
Atlantic salmon Salmo salar |
genre_facet |
Atlantic salmon Salmo salar |
op_source |
Microorganisms; Volume 9; Issue 4; Pages: 746 |
op_relation |
Parasitology https://dx.doi.org/10.3390/microorganisms9040746 |
op_rights |
https://creativecommons.org/licenses/by/4.0/ |
op_doi |
https://doi.org/10.3390/microorganisms9040746 |
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Microorganisms |
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9 |
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4 |
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746 |
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1774715757596246016 |