Microbial Communities Involved in Methane, Sulfur, and Nitrogen Cycling in the Sediments of the Barents Sea
A combination of physicochemical and radiotracer analysis, high-throughput sequencing of the 16S rRNA, and particulate methane monooxygenase subunit A (pmoA) genes was used to link a microbial community profile with methane, sulfur, and nitrogen cycling processes. The objects of study were surface s...
Published in: | Microorganisms |
---|---|
Main Authors: | , , , , , , , , , |
Format: | Text |
Language: | English |
Published: |
Multidisciplinary Digital Publishing Institute
2021
|
Subjects: | |
Online Access: | https://doi.org/10.3390/microorganisms9112362 |
id |
ftmdpi:oai:mdpi.com:/2076-2607/9/11/2362/ |
---|---|
record_format |
openpolar |
spelling |
ftmdpi:oai:mdpi.com:/2076-2607/9/11/2362/ 2023-08-20T04:04:29+02:00 Microbial Communities Involved in Methane, Sulfur, and Nitrogen Cycling in the Sediments of the Barents Sea Shahjahon Begmatov Alexander S. Savvichev Vitaly V. Kadnikov Alexey V. Beletsky Igor I. Rusanov Alexey A. Klyuvitkin Ekaterina A. Novichkova Andrey V. Mardanov Nikolai V. Pimenov Nikolai V. Ravin agris 2021-11-15 application/pdf https://doi.org/10.3390/microorganisms9112362 EN eng Multidisciplinary Digital Publishing Institute Environmental Microbiology https://dx.doi.org/10.3390/microorganisms9112362 https://creativecommons.org/licenses/by/4.0/ Microorganisms; Volume 9; Issue 11; Pages: 2362 arctic marine sediments methane cycle sulfur cycle nitrogen cycle microbial communities Barents Sea Text 2021 ftmdpi https://doi.org/10.3390/microorganisms9112362 2023-08-01T03:15:42Z A combination of physicochemical and radiotracer analysis, high-throughput sequencing of the 16S rRNA, and particulate methane monooxygenase subunit A (pmoA) genes was used to link a microbial community profile with methane, sulfur, and nitrogen cycling processes. The objects of study were surface sediments sampled at five stations in the northern part of the Barents Sea. The methane content in the upper layers (0–5 cm) ranged from 0.2 to 2.4 µM and increased with depth (16–19 cm) to 9.5 µM. The rate of methane oxidation in the oxic upper layers varied from 2 to 23 nmol CH4 L−1 day−1 and decreased to 0.3 nmol L−1 day−1 in the anoxic zone at a depth of 16–19 cm. Sulfate reduction rates were much higher, from 0.3 to 2.8 µmol L−1 day−1. In the surface sediments, ammonia-oxidizing Nitrosopumilaceae were abundant; the subsequent oxidation of nitrite to nitrate can be carried out by Nitrospira sp. Aerobic methane oxidation could be performed by uncultured deep-sea cluster 3 of gamma-proteobacterial methanotrophs. Undetectable low levels of methanogenesis were consistent with a near complete absence of methanogens. Anaerobic methane oxidation in the deeper sediments was likely performed by ANME-2a-2b and ANME-2c archaea in consortium with sulfate-reducing Desulfobacterota. Sulfide can be oxidized by nitrate-reducing Sulfurovum sp. Thus, the sulfur cycle was linked with the anaerobic oxidation of methane and the nitrogen cycle, which included the oxidation of ammonium to nitrate in the oxic zone and denitrification coupled to the oxidation of sulfide in the deeper sediments. Methane concentrations and rates of microbial biogeochemical processes in sediments in the northern part of the Barents Sea were noticeably higher than in oligotrophic areas of the Arctic Ocean, indicating that an increase in methane concentration significantly activates microbial processes. Text Arctic Arctic Ocean Barents Sea MDPI Open Access Publishing Arctic Arctic Ocean Barents Sea Microorganisms 9 11 2362 |
institution |
Open Polar |
collection |
MDPI Open Access Publishing |
op_collection_id |
ftmdpi |
language |
English |
topic |
arctic marine sediments methane cycle sulfur cycle nitrogen cycle microbial communities Barents Sea |
spellingShingle |
arctic marine sediments methane cycle sulfur cycle nitrogen cycle microbial communities Barents Sea Shahjahon Begmatov Alexander S. Savvichev Vitaly V. Kadnikov Alexey V. Beletsky Igor I. Rusanov Alexey A. Klyuvitkin Ekaterina A. Novichkova Andrey V. Mardanov Nikolai V. Pimenov Nikolai V. Ravin Microbial Communities Involved in Methane, Sulfur, and Nitrogen Cycling in the Sediments of the Barents Sea |
topic_facet |
arctic marine sediments methane cycle sulfur cycle nitrogen cycle microbial communities Barents Sea |
description |
A combination of physicochemical and radiotracer analysis, high-throughput sequencing of the 16S rRNA, and particulate methane monooxygenase subunit A (pmoA) genes was used to link a microbial community profile with methane, sulfur, and nitrogen cycling processes. The objects of study were surface sediments sampled at five stations in the northern part of the Barents Sea. The methane content in the upper layers (0–5 cm) ranged from 0.2 to 2.4 µM and increased with depth (16–19 cm) to 9.5 µM. The rate of methane oxidation in the oxic upper layers varied from 2 to 23 nmol CH4 L−1 day−1 and decreased to 0.3 nmol L−1 day−1 in the anoxic zone at a depth of 16–19 cm. Sulfate reduction rates were much higher, from 0.3 to 2.8 µmol L−1 day−1. In the surface sediments, ammonia-oxidizing Nitrosopumilaceae were abundant; the subsequent oxidation of nitrite to nitrate can be carried out by Nitrospira sp. Aerobic methane oxidation could be performed by uncultured deep-sea cluster 3 of gamma-proteobacterial methanotrophs. Undetectable low levels of methanogenesis were consistent with a near complete absence of methanogens. Anaerobic methane oxidation in the deeper sediments was likely performed by ANME-2a-2b and ANME-2c archaea in consortium with sulfate-reducing Desulfobacterota. Sulfide can be oxidized by nitrate-reducing Sulfurovum sp. Thus, the sulfur cycle was linked with the anaerobic oxidation of methane and the nitrogen cycle, which included the oxidation of ammonium to nitrate in the oxic zone and denitrification coupled to the oxidation of sulfide in the deeper sediments. Methane concentrations and rates of microbial biogeochemical processes in sediments in the northern part of the Barents Sea were noticeably higher than in oligotrophic areas of the Arctic Ocean, indicating that an increase in methane concentration significantly activates microbial processes. |
format |
Text |
author |
Shahjahon Begmatov Alexander S. Savvichev Vitaly V. Kadnikov Alexey V. Beletsky Igor I. Rusanov Alexey A. Klyuvitkin Ekaterina A. Novichkova Andrey V. Mardanov Nikolai V. Pimenov Nikolai V. Ravin |
author_facet |
Shahjahon Begmatov Alexander S. Savvichev Vitaly V. Kadnikov Alexey V. Beletsky Igor I. Rusanov Alexey A. Klyuvitkin Ekaterina A. Novichkova Andrey V. Mardanov Nikolai V. Pimenov Nikolai V. Ravin |
author_sort |
Shahjahon Begmatov |
title |
Microbial Communities Involved in Methane, Sulfur, and Nitrogen Cycling in the Sediments of the Barents Sea |
title_short |
Microbial Communities Involved in Methane, Sulfur, and Nitrogen Cycling in the Sediments of the Barents Sea |
title_full |
Microbial Communities Involved in Methane, Sulfur, and Nitrogen Cycling in the Sediments of the Barents Sea |
title_fullStr |
Microbial Communities Involved in Methane, Sulfur, and Nitrogen Cycling in the Sediments of the Barents Sea |
title_full_unstemmed |
Microbial Communities Involved in Methane, Sulfur, and Nitrogen Cycling in the Sediments of the Barents Sea |
title_sort |
microbial communities involved in methane, sulfur, and nitrogen cycling in the sediments of the barents sea |
publisher |
Multidisciplinary Digital Publishing Institute |
publishDate |
2021 |
url |
https://doi.org/10.3390/microorganisms9112362 |
op_coverage |
agris |
geographic |
Arctic Arctic Ocean Barents Sea |
geographic_facet |
Arctic Arctic Ocean Barents Sea |
genre |
Arctic Arctic Ocean Barents Sea |
genre_facet |
Arctic Arctic Ocean Barents Sea |
op_source |
Microorganisms; Volume 9; Issue 11; Pages: 2362 |
op_relation |
Environmental Microbiology https://dx.doi.org/10.3390/microorganisms9112362 |
op_rights |
https://creativecommons.org/licenses/by/4.0/ |
op_doi |
https://doi.org/10.3390/microorganisms9112362 |
container_title |
Microorganisms |
container_volume |
9 |
container_issue |
11 |
container_start_page |
2362 |
_version_ |
1774714855714979840 |