Hot in Cold: Microbial Life in the Hottest Springs in Permafrost
Chukotka is an arctic region located in the continuous permafrost zone, but thermal springs are abundant there. In this study, for the first time, the microbial communities of the Chukotka hot springs (CHS) biofilms and sediments with temperatures 54–94 °C were investigated and analyzed by NGS seque...
Published in: | Microorganisms |
---|---|
Main Authors: | , , , , , , , |
Format: | Text |
Language: | English |
Published: |
Multidisciplinary Digital Publishing Institute
2020
|
Subjects: | |
Online Access: | https://doi.org/10.3390/microorganisms8091308 |
id |
ftmdpi:oai:mdpi.com:/2076-2607/8/9/1308/ |
---|---|
record_format |
openpolar |
spelling |
ftmdpi:oai:mdpi.com:/2076-2607/8/9/1308/ 2023-08-20T04:04:41+02:00 Hot in Cold: Microbial Life in the Hottest Springs in Permafrost Tatiana V. Kochetkova Stepan V. Toshchakov Kseniya S. Zayulina Alexander G. Elcheninov Daria G. Zavarzina Vasiliy Yu. Lavrushin Elizaveta A. Bonch-Osmolovskaya Ilya V. Kublanov agris 2020-08-27 application/pdf https://doi.org/10.3390/microorganisms8091308 EN eng Multidisciplinary Digital Publishing Institute Environmental Microbiology https://dx.doi.org/10.3390/microorganisms8091308 https://creativecommons.org/licenses/by/4.0/ Microorganisms; Volume 8; Issue 9; Pages: 1308 Chukotka thermophiles hot spring NGS sequencing microbial diversity permafrost polar environments Text 2020 ftmdpi https://doi.org/10.3390/microorganisms8091308 2023-07-31T23:59:42Z Chukotka is an arctic region located in the continuous permafrost zone, but thermal springs are abundant there. In this study, for the first time, the microbial communities of the Chukotka hot springs (CHS) biofilms and sediments with temperatures 54–94 °C were investigated and analyzed by NGS sequencing of 16S rRNA gene amplicons. In microbial mats (54–75 °C), phototrophic bacteria of genus Chloroflexus dominated (up to 89% of all prokaryotes), while Aquificae were the most numerous at higher temperatures in Fe-rich sediments and filamentous “streamers” (up to 92%). The electron donors typical for Aquificae, such as H2S and H2, are absent or present only in trace amounts, and the prevalence of Aquificae might be connected with their ability to oxidize the ferrous iron present in CHS sediments. Armatimonadetes, Proteobacteria, Deinococcus-Thermus, Dictyoglomi, and Thermotogae, as well as uncultured bacteria (candidate divisions Oct-Spa1-106, GAL15, and OPB56), were numerous, and Cyanobacteria were present in low numbers. Archaea (less than 8% of the total community of each tested spring) belonged to Bathyarchaeota, Aigarchaeota, and Thaumarchaeota. The geographical location and the predominantly autotrophic microbial community, built on mechanisms other than the sulfur cycle-based ones, make CHS a special and unique terrestrial geothermal ecosystem. Text Arctic Chukotka permafrost MDPI Open Access Publishing Arctic Microorganisms 8 9 1308 |
institution |
Open Polar |
collection |
MDPI Open Access Publishing |
op_collection_id |
ftmdpi |
language |
English |
topic |
Chukotka thermophiles hot spring NGS sequencing microbial diversity permafrost polar environments |
spellingShingle |
Chukotka thermophiles hot spring NGS sequencing microbial diversity permafrost polar environments Tatiana V. Kochetkova Stepan V. Toshchakov Kseniya S. Zayulina Alexander G. Elcheninov Daria G. Zavarzina Vasiliy Yu. Lavrushin Elizaveta A. Bonch-Osmolovskaya Ilya V. Kublanov Hot in Cold: Microbial Life in the Hottest Springs in Permafrost |
topic_facet |
Chukotka thermophiles hot spring NGS sequencing microbial diversity permafrost polar environments |
description |
Chukotka is an arctic region located in the continuous permafrost zone, but thermal springs are abundant there. In this study, for the first time, the microbial communities of the Chukotka hot springs (CHS) biofilms and sediments with temperatures 54–94 °C were investigated and analyzed by NGS sequencing of 16S rRNA gene amplicons. In microbial mats (54–75 °C), phototrophic bacteria of genus Chloroflexus dominated (up to 89% of all prokaryotes), while Aquificae were the most numerous at higher temperatures in Fe-rich sediments and filamentous “streamers” (up to 92%). The electron donors typical for Aquificae, such as H2S and H2, are absent or present only in trace amounts, and the prevalence of Aquificae might be connected with their ability to oxidize the ferrous iron present in CHS sediments. Armatimonadetes, Proteobacteria, Deinococcus-Thermus, Dictyoglomi, and Thermotogae, as well as uncultured bacteria (candidate divisions Oct-Spa1-106, GAL15, and OPB56), were numerous, and Cyanobacteria were present in low numbers. Archaea (less than 8% of the total community of each tested spring) belonged to Bathyarchaeota, Aigarchaeota, and Thaumarchaeota. The geographical location and the predominantly autotrophic microbial community, built on mechanisms other than the sulfur cycle-based ones, make CHS a special and unique terrestrial geothermal ecosystem. |
format |
Text |
author |
Tatiana V. Kochetkova Stepan V. Toshchakov Kseniya S. Zayulina Alexander G. Elcheninov Daria G. Zavarzina Vasiliy Yu. Lavrushin Elizaveta A. Bonch-Osmolovskaya Ilya V. Kublanov |
author_facet |
Tatiana V. Kochetkova Stepan V. Toshchakov Kseniya S. Zayulina Alexander G. Elcheninov Daria G. Zavarzina Vasiliy Yu. Lavrushin Elizaveta A. Bonch-Osmolovskaya Ilya V. Kublanov |
author_sort |
Tatiana V. Kochetkova |
title |
Hot in Cold: Microbial Life in the Hottest Springs in Permafrost |
title_short |
Hot in Cold: Microbial Life in the Hottest Springs in Permafrost |
title_full |
Hot in Cold: Microbial Life in the Hottest Springs in Permafrost |
title_fullStr |
Hot in Cold: Microbial Life in the Hottest Springs in Permafrost |
title_full_unstemmed |
Hot in Cold: Microbial Life in the Hottest Springs in Permafrost |
title_sort |
hot in cold: microbial life in the hottest springs in permafrost |
publisher |
Multidisciplinary Digital Publishing Institute |
publishDate |
2020 |
url |
https://doi.org/10.3390/microorganisms8091308 |
op_coverage |
agris |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic Chukotka permafrost |
genre_facet |
Arctic Chukotka permafrost |
op_source |
Microorganisms; Volume 8; Issue 9; Pages: 1308 |
op_relation |
Environmental Microbiology https://dx.doi.org/10.3390/microorganisms8091308 |
op_rights |
https://creativecommons.org/licenses/by/4.0/ |
op_doi |
https://doi.org/10.3390/microorganisms8091308 |
container_title |
Microorganisms |
container_volume |
8 |
container_issue |
9 |
container_start_page |
1308 |
_version_ |
1774715063821664256 |