Antibiotic Resistance Genes in Phage Particles from Antarctic and Mediterranean Seawater Ecosystems
Anthropogenic activities are a key factor in the development of antibiotic resistance in bacteria, a growing problem worldwide. Nevertheless, antibiotics and resistances were being generated by bacterial communities long before their discovery by humankind, and might occur in areas without human inf...
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ftmdpi:oai:mdpi.com:/2076-2607/8/9/1293/ 2023-08-20T04:02:31+02:00 Antibiotic Resistance Genes in Phage Particles from Antarctic and Mediterranean Seawater Ecosystems Pedro Blanco-Picazo Gabriel Roscales Daniel Toribio-Avedillo Clara Gómez-Gómez Conxita Avila Elisenda Ballesté Maite Muniesa Lorena Rodríguez-Rubio agris 2020-08-24 application/pdf https://doi.org/10.3390/microorganisms8091293 EN eng Multidisciplinary Digital Publishing Institute Molecular Microbiology and Immunology https://dx.doi.org/10.3390/microorganisms8091293 https://creativecommons.org/licenses/by/4.0/ Microorganisms; Volume 8; Issue 9; Pages: 1293 bacteriophages shellfish fish transduction ARG horizontal gene transfer Text 2020 ftmdpi https://doi.org/10.3390/microorganisms8091293 2023-07-31T23:58:31Z Anthropogenic activities are a key factor in the development of antibiotic resistance in bacteria, a growing problem worldwide. Nevertheless, antibiotics and resistances were being generated by bacterial communities long before their discovery by humankind, and might occur in areas without human influence. Bacteriophages are known to play a relevant role in the dissemination of antibiotic resistance genes (ARGs) in aquatic environments. In this study, five ARGs (blaTEM, blaCTX-M-1, blaCTX-M-9, sul1 and tetW) were monitored in phage particles isolated from seawater of two different locations: (i) the Mediterranean coast, subjected to high anthropogenic pressure, and (ii) the Antarctic coast, where the anthropogenic impact is low. Although found in lower quantities, ARG-containing phage particles were more prevalent among the Antarctic than the Mediterranean seawater samples and Antarctic bacterial communities were confirmed as their source. In the Mediterranean area, ARG-containing phages from anthropogenic fecal pollution might allow ARG transmission through the food chain. ARGs were detected in phage particles isolated from fish (Mediterranean, Atlantic, farmed, and frozen), the most abundant being β-lactamases. Some of these particles were infectious in cultures of the fecal bacteria Escherichia coli. By serving as ARG reservoirs in marine environments, including those with low human activity, such as the Antarctic, phages could contribute to ARG transmission between bacterial communities. Text Antarc* Antarctic MDPI Open Access Publishing Antarctic The Antarctic Microorganisms 8 9 1293 |
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MDPI Open Access Publishing |
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English |
topic |
bacteriophages shellfish fish transduction ARG horizontal gene transfer |
spellingShingle |
bacteriophages shellfish fish transduction ARG horizontal gene transfer Pedro Blanco-Picazo Gabriel Roscales Daniel Toribio-Avedillo Clara Gómez-Gómez Conxita Avila Elisenda Ballesté Maite Muniesa Lorena Rodríguez-Rubio Antibiotic Resistance Genes in Phage Particles from Antarctic and Mediterranean Seawater Ecosystems |
topic_facet |
bacteriophages shellfish fish transduction ARG horizontal gene transfer |
description |
Anthropogenic activities are a key factor in the development of antibiotic resistance in bacteria, a growing problem worldwide. Nevertheless, antibiotics and resistances were being generated by bacterial communities long before their discovery by humankind, and might occur in areas without human influence. Bacteriophages are known to play a relevant role in the dissemination of antibiotic resistance genes (ARGs) in aquatic environments. In this study, five ARGs (blaTEM, blaCTX-M-1, blaCTX-M-9, sul1 and tetW) were monitored in phage particles isolated from seawater of two different locations: (i) the Mediterranean coast, subjected to high anthropogenic pressure, and (ii) the Antarctic coast, where the anthropogenic impact is low. Although found in lower quantities, ARG-containing phage particles were more prevalent among the Antarctic than the Mediterranean seawater samples and Antarctic bacterial communities were confirmed as their source. In the Mediterranean area, ARG-containing phages from anthropogenic fecal pollution might allow ARG transmission through the food chain. ARGs were detected in phage particles isolated from fish (Mediterranean, Atlantic, farmed, and frozen), the most abundant being β-lactamases. Some of these particles were infectious in cultures of the fecal bacteria Escherichia coli. By serving as ARG reservoirs in marine environments, including those with low human activity, such as the Antarctic, phages could contribute to ARG transmission between bacterial communities. |
format |
Text |
author |
Pedro Blanco-Picazo Gabriel Roscales Daniel Toribio-Avedillo Clara Gómez-Gómez Conxita Avila Elisenda Ballesté Maite Muniesa Lorena Rodríguez-Rubio |
author_facet |
Pedro Blanco-Picazo Gabriel Roscales Daniel Toribio-Avedillo Clara Gómez-Gómez Conxita Avila Elisenda Ballesté Maite Muniesa Lorena Rodríguez-Rubio |
author_sort |
Pedro Blanco-Picazo |
title |
Antibiotic Resistance Genes in Phage Particles from Antarctic and Mediterranean Seawater Ecosystems |
title_short |
Antibiotic Resistance Genes in Phage Particles from Antarctic and Mediterranean Seawater Ecosystems |
title_full |
Antibiotic Resistance Genes in Phage Particles from Antarctic and Mediterranean Seawater Ecosystems |
title_fullStr |
Antibiotic Resistance Genes in Phage Particles from Antarctic and Mediterranean Seawater Ecosystems |
title_full_unstemmed |
Antibiotic Resistance Genes in Phage Particles from Antarctic and Mediterranean Seawater Ecosystems |
title_sort |
antibiotic resistance genes in phage particles from antarctic and mediterranean seawater ecosystems |
publisher |
Multidisciplinary Digital Publishing Institute |
publishDate |
2020 |
url |
https://doi.org/10.3390/microorganisms8091293 |
op_coverage |
agris |
geographic |
Antarctic The Antarctic |
geographic_facet |
Antarctic The Antarctic |
genre |
Antarc* Antarctic |
genre_facet |
Antarc* Antarctic |
op_source |
Microorganisms; Volume 8; Issue 9; Pages: 1293 |
op_relation |
Molecular Microbiology and Immunology https://dx.doi.org/10.3390/microorganisms8091293 |
op_rights |
https://creativecommons.org/licenses/by/4.0/ |
op_doi |
https://doi.org/10.3390/microorganisms8091293 |
container_title |
Microorganisms |
container_volume |
8 |
container_issue |
9 |
container_start_page |
1293 |
_version_ |
1774712994340536320 |