Novel Psychrophiles and Exopolymers from Permafrost Thaw Lake Sediments
Thermokarst lakes are one of the most abundant types of microbial ecosystems in the circumpolar North. These shallow basins are formed by the thawing and collapse of ice-rich permafrost, with subsequent filling by snow and ice melt. Until now, permafrost thaw lakes have received little attention for...
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ftmdpi:oai:mdpi.com:/2076-2607/8/9/1282/ 2023-08-20T04:07:07+02:00 Novel Psychrophiles and Exopolymers from Permafrost Thaw Lake Sediments Ilaria Finore Adrien Vigneron Warwick F. Vincent Luigi Leone Paola Di Donato Aniello Schiano Moriello Barbara Nicolaus Annarita Poli agris 2020-08-22 application/pdf https://doi.org/10.3390/microorganisms8091282 EN eng Multidisciplinary Digital Publishing Institute Environmental Microbiology https://dx.doi.org/10.3390/microorganisms8091282 https://creativecommons.org/licenses/by/4.0/ Microorganisms; Volume 8; Issue 9; Pages: 1282 bacterial diversity exopolysaccharides lake sediments microbial ecology permafrost thaw lake subarctic psychrophiles Pseudomonas Text 2020 ftmdpi https://doi.org/10.3390/microorganisms8091282 2023-07-31T23:58:08Z Thermokarst lakes are one of the most abundant types of microbial ecosystems in the circumpolar North. These shallow basins are formed by the thawing and collapse of ice-rich permafrost, with subsequent filling by snow and ice melt. Until now, permafrost thaw lakes have received little attention for isolation of microorganisms by culture-based analysis. The discovery of novel psychrophiles and their biomolecules makes these extreme environments suitable sources for the isolation of new strains, including for potential biotechnological applications. In this study, samples of bottom sediments were collected from three permafrost thaw lakes in subarctic Québec, Canada. Their diverse microbial communities were characterized by 16S rRNA gene amplicon analysis, and subsamples were cultured for the isolation of bacterial strains. Phenotypic and genetic characterization of the isolates revealed affinities to the genera Pseudomonas, Paenibacillus, Acinetobacter,Staphylococcus and Sphingomonas. The isolates were then evaluated for their production of extracellular enzymes and exopolymers. Enzymes of potential biotechnological interest included α and β-glucosidase, α and β-maltosidase, β-xylosidase and cellobiohydrolase. One isolate, Pseudomonas extremaustralis strain 2ASCA, also showed the capability to produce, in the loosely bound cell fraction, a levan-type polysaccharide with a yield of 613 mg/L of culture, suggesting its suitability as a candidate for eco-sustainable alternatives to commercial polymers. Text Ice permafrost Subarctic Thermokarst MDPI Open Access Publishing Canada Microorganisms 8 9 1282 |
institution |
Open Polar |
collection |
MDPI Open Access Publishing |
op_collection_id |
ftmdpi |
language |
English |
topic |
bacterial diversity exopolysaccharides lake sediments microbial ecology permafrost thaw lake subarctic psychrophiles Pseudomonas |
spellingShingle |
bacterial diversity exopolysaccharides lake sediments microbial ecology permafrost thaw lake subarctic psychrophiles Pseudomonas Ilaria Finore Adrien Vigneron Warwick F. Vincent Luigi Leone Paola Di Donato Aniello Schiano Moriello Barbara Nicolaus Annarita Poli Novel Psychrophiles and Exopolymers from Permafrost Thaw Lake Sediments |
topic_facet |
bacterial diversity exopolysaccharides lake sediments microbial ecology permafrost thaw lake subarctic psychrophiles Pseudomonas |
description |
Thermokarst lakes are one of the most abundant types of microbial ecosystems in the circumpolar North. These shallow basins are formed by the thawing and collapse of ice-rich permafrost, with subsequent filling by snow and ice melt. Until now, permafrost thaw lakes have received little attention for isolation of microorganisms by culture-based analysis. The discovery of novel psychrophiles and their biomolecules makes these extreme environments suitable sources for the isolation of new strains, including for potential biotechnological applications. In this study, samples of bottom sediments were collected from three permafrost thaw lakes in subarctic Québec, Canada. Their diverse microbial communities were characterized by 16S rRNA gene amplicon analysis, and subsamples were cultured for the isolation of bacterial strains. Phenotypic and genetic characterization of the isolates revealed affinities to the genera Pseudomonas, Paenibacillus, Acinetobacter,Staphylococcus and Sphingomonas. The isolates were then evaluated for their production of extracellular enzymes and exopolymers. Enzymes of potential biotechnological interest included α and β-glucosidase, α and β-maltosidase, β-xylosidase and cellobiohydrolase. One isolate, Pseudomonas extremaustralis strain 2ASCA, also showed the capability to produce, in the loosely bound cell fraction, a levan-type polysaccharide with a yield of 613 mg/L of culture, suggesting its suitability as a candidate for eco-sustainable alternatives to commercial polymers. |
format |
Text |
author |
Ilaria Finore Adrien Vigneron Warwick F. Vincent Luigi Leone Paola Di Donato Aniello Schiano Moriello Barbara Nicolaus Annarita Poli |
author_facet |
Ilaria Finore Adrien Vigneron Warwick F. Vincent Luigi Leone Paola Di Donato Aniello Schiano Moriello Barbara Nicolaus Annarita Poli |
author_sort |
Ilaria Finore |
title |
Novel Psychrophiles and Exopolymers from Permafrost Thaw Lake Sediments |
title_short |
Novel Psychrophiles and Exopolymers from Permafrost Thaw Lake Sediments |
title_full |
Novel Psychrophiles and Exopolymers from Permafrost Thaw Lake Sediments |
title_fullStr |
Novel Psychrophiles and Exopolymers from Permafrost Thaw Lake Sediments |
title_full_unstemmed |
Novel Psychrophiles and Exopolymers from Permafrost Thaw Lake Sediments |
title_sort |
novel psychrophiles and exopolymers from permafrost thaw lake sediments |
publisher |
Multidisciplinary Digital Publishing Institute |
publishDate |
2020 |
url |
https://doi.org/10.3390/microorganisms8091282 |
op_coverage |
agris |
geographic |
Canada |
geographic_facet |
Canada |
genre |
Ice permafrost Subarctic Thermokarst |
genre_facet |
Ice permafrost Subarctic Thermokarst |
op_source |
Microorganisms; Volume 8; Issue 9; Pages: 1282 |
op_relation |
Environmental Microbiology https://dx.doi.org/10.3390/microorganisms8091282 |
op_rights |
https://creativecommons.org/licenses/by/4.0/ |
op_doi |
https://doi.org/10.3390/microorganisms8091282 |
container_title |
Microorganisms |
container_volume |
8 |
container_issue |
9 |
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1282 |
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1774718561053310976 |