Antimicrobial Resistance in Members of the Bacterial Bovine Respiratory Disease Complex Isolated from Lung Tissue of Cattle Mortalities Managed with or without the Use of Antimicrobials
Over a two-year period, Mannheimia haemolytica (MH; n = 113), Pasteurella multocida (PM; n = 47), Histophilus somni (HS; n = 41) and Mycoplasma bovis (MB; n = 227) were isolated from bovine lung tissue at necropsy from cattle raised conventionally (CON, n = 29 feedlots) or without antimicrobials [na...
Published in: | Microorganisms |
---|---|
Main Authors: | , , , , , , |
Format: | Text |
Language: | English |
Published: |
Multidisciplinary Digital Publishing Institute
2020
|
Subjects: | |
Online Access: | https://doi.org/10.3390/microorganisms8020288 |
id |
ftmdpi:oai:mdpi.com:/2076-2607/8/2/288/ |
---|---|
record_format |
openpolar |
spelling |
ftmdpi:oai:mdpi.com:/2076-2607/8/2/288/ 2023-08-20T04:07:11+02:00 Antimicrobial Resistance in Members of the Bacterial Bovine Respiratory Disease Complex Isolated from Lung Tissue of Cattle Mortalities Managed with or without the Use of Antimicrobials Kim Stanford Rahat Zaheer Cassidy Klima Tim McAllister Delores Peters Yan D. Niu Brenda Ralston agris 2020-02-20 application/pdf https://doi.org/10.3390/microorganisms8020288 EN eng Multidisciplinary Digital Publishing Institute Antimicrobial Agents and Resistance https://dx.doi.org/10.3390/microorganisms8020288 https://creativecommons.org/licenses/by/4.0/ Microorganisms; Volume 8; Issue 2; Pages: 288 Mannheimia haemolytica Pasteurella multocida Histophilus somni Mycoplasma bovis antimicrobial resistance managed without antimicrobials bovine respiratory disease Text 2020 ftmdpi https://doi.org/10.3390/microorganisms8020288 2023-07-31T23:08:31Z Over a two-year period, Mannheimia haemolytica (MH; n = 113), Pasteurella multocida (PM; n = 47), Histophilus somni (HS; n = 41) and Mycoplasma bovis (MB; n = 227) were isolated from bovine lung tissue at necropsy from cattle raised conventionally (CON, n = 29 feedlots) or without antimicrobials [natural (NAT), n = 2 feedlots]. Excluding MB, isolates were assayed by PCR to detect the presence of 13 antimicrobial resistance (AMR) genes and five core genes associated with integrative and conjugative elements (ICEs). Antimicrobial susceptibility phenotypes and minimum inhibitory concentrations (MICs, µg/mL) were determined for a subset of isolates (MH, n = 104; PM, n = 45; HS, n = 23; and MB, n = 61) using Sensititre analyses. A subset of isolates (n = 21) was also evaluated by whole-genome sequencing (WGS) based on variation in AMR phenotype. All five ICE core genes were detected in PM and HS by PCR, but only 3/5 were present in MH. Presence of mco and tnpA ICE core genes in MH was associated with higher MICs (p < 0.05) for all tetracyclines, and 2/3 of all macrolides, aminoglycosides and fluoroquinolones evaluated. In contrast, association of ICE core genes with MICs was largely restricted to macrolides for PM and to individual tetracyclines and macrolides for HS. For MH, the average number of AMR genes markedly increased (p < 0.05) in year 2 of the study due to the emergence of a strain that was PCR positive for all 13 PCR-tested AMR genes as well as two additional AMR genes (aadA31 and blaROB-1) detected by WGS. Conventional management of cattle increased (p < 0.05) MICs of tilmicosin and tulathromycin for MH; neomycin and spectinomycin for PM; and gamithromycin and tulathromycin for MB. The average number of PCR-detected AMR genes in PM was also increased (p < 0.05) in CON mortalities. This study demonstrates increased AMR especially to macrolides by bovine respiratory disease organisms in CON as compared to NAT feedlots and a rapid increase in AMR following dissemination of strain(s) carrying ... Text ice core MDPI Open Access Publishing Microorganisms 8 2 288 |
institution |
Open Polar |
collection |
MDPI Open Access Publishing |
op_collection_id |
ftmdpi |
language |
English |
topic |
Mannheimia haemolytica Pasteurella multocida Histophilus somni Mycoplasma bovis antimicrobial resistance managed without antimicrobials bovine respiratory disease |
spellingShingle |
Mannheimia haemolytica Pasteurella multocida Histophilus somni Mycoplasma bovis antimicrobial resistance managed without antimicrobials bovine respiratory disease Kim Stanford Rahat Zaheer Cassidy Klima Tim McAllister Delores Peters Yan D. Niu Brenda Ralston Antimicrobial Resistance in Members of the Bacterial Bovine Respiratory Disease Complex Isolated from Lung Tissue of Cattle Mortalities Managed with or without the Use of Antimicrobials |
topic_facet |
Mannheimia haemolytica Pasteurella multocida Histophilus somni Mycoplasma bovis antimicrobial resistance managed without antimicrobials bovine respiratory disease |
description |
Over a two-year period, Mannheimia haemolytica (MH; n = 113), Pasteurella multocida (PM; n = 47), Histophilus somni (HS; n = 41) and Mycoplasma bovis (MB; n = 227) were isolated from bovine lung tissue at necropsy from cattle raised conventionally (CON, n = 29 feedlots) or without antimicrobials [natural (NAT), n = 2 feedlots]. Excluding MB, isolates were assayed by PCR to detect the presence of 13 antimicrobial resistance (AMR) genes and five core genes associated with integrative and conjugative elements (ICEs). Antimicrobial susceptibility phenotypes and minimum inhibitory concentrations (MICs, µg/mL) were determined for a subset of isolates (MH, n = 104; PM, n = 45; HS, n = 23; and MB, n = 61) using Sensititre analyses. A subset of isolates (n = 21) was also evaluated by whole-genome sequencing (WGS) based on variation in AMR phenotype. All five ICE core genes were detected in PM and HS by PCR, but only 3/5 were present in MH. Presence of mco and tnpA ICE core genes in MH was associated with higher MICs (p < 0.05) for all tetracyclines, and 2/3 of all macrolides, aminoglycosides and fluoroquinolones evaluated. In contrast, association of ICE core genes with MICs was largely restricted to macrolides for PM and to individual tetracyclines and macrolides for HS. For MH, the average number of AMR genes markedly increased (p < 0.05) in year 2 of the study due to the emergence of a strain that was PCR positive for all 13 PCR-tested AMR genes as well as two additional AMR genes (aadA31 and blaROB-1) detected by WGS. Conventional management of cattle increased (p < 0.05) MICs of tilmicosin and tulathromycin for MH; neomycin and spectinomycin for PM; and gamithromycin and tulathromycin for MB. The average number of PCR-detected AMR genes in PM was also increased (p < 0.05) in CON mortalities. This study demonstrates increased AMR especially to macrolides by bovine respiratory disease organisms in CON as compared to NAT feedlots and a rapid increase in AMR following dissemination of strain(s) carrying ... |
format |
Text |
author |
Kim Stanford Rahat Zaheer Cassidy Klima Tim McAllister Delores Peters Yan D. Niu Brenda Ralston |
author_facet |
Kim Stanford Rahat Zaheer Cassidy Klima Tim McAllister Delores Peters Yan D. Niu Brenda Ralston |
author_sort |
Kim Stanford |
title |
Antimicrobial Resistance in Members of the Bacterial Bovine Respiratory Disease Complex Isolated from Lung Tissue of Cattle Mortalities Managed with or without the Use of Antimicrobials |
title_short |
Antimicrobial Resistance in Members of the Bacterial Bovine Respiratory Disease Complex Isolated from Lung Tissue of Cattle Mortalities Managed with or without the Use of Antimicrobials |
title_full |
Antimicrobial Resistance in Members of the Bacterial Bovine Respiratory Disease Complex Isolated from Lung Tissue of Cattle Mortalities Managed with or without the Use of Antimicrobials |
title_fullStr |
Antimicrobial Resistance in Members of the Bacterial Bovine Respiratory Disease Complex Isolated from Lung Tissue of Cattle Mortalities Managed with or without the Use of Antimicrobials |
title_full_unstemmed |
Antimicrobial Resistance in Members of the Bacterial Bovine Respiratory Disease Complex Isolated from Lung Tissue of Cattle Mortalities Managed with or without the Use of Antimicrobials |
title_sort |
antimicrobial resistance in members of the bacterial bovine respiratory disease complex isolated from lung tissue of cattle mortalities managed with or without the use of antimicrobials |
publisher |
Multidisciplinary Digital Publishing Institute |
publishDate |
2020 |
url |
https://doi.org/10.3390/microorganisms8020288 |
op_coverage |
agris |
genre |
ice core |
genre_facet |
ice core |
op_source |
Microorganisms; Volume 8; Issue 2; Pages: 288 |
op_relation |
Antimicrobial Agents and Resistance https://dx.doi.org/10.3390/microorganisms8020288 |
op_rights |
https://creativecommons.org/licenses/by/4.0/ |
op_doi |
https://doi.org/10.3390/microorganisms8020288 |
container_title |
Microorganisms |
container_volume |
8 |
container_issue |
2 |
container_start_page |
288 |
_version_ |
1774718661578194944 |