Diversity of Bacterial Biosynthetic Genes in Maritime Antarctica
Bacterial natural products (NPs) are still a major source of new drug leads. Polyketides (PKs) and non-ribosomal peptides (NRP) are two pharmaceutically important families of NPs and recent studies have revealed Antarctica to harbor endemic polyketide synthase (PKS) and non-ribosomal peptide synthet...
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ftmdpi:oai:mdpi.com:/2076-2607/8/2/279/ 2023-08-20T04:01:15+02:00 Diversity of Bacterial Biosynthetic Genes in Maritime Antarctica Adriana Rego António G. G. Sousa João P. Santos Francisco Pascoal João Canário Pedro N. Leão Catarina Magalhães agris 2020-02-18 application/pdf https://doi.org/10.3390/microorganisms8020279 EN eng Multidisciplinary Digital Publishing Institute Environmental Microbiology https://dx.doi.org/10.3390/microorganisms8020279 https://creativecommons.org/licenses/by/4.0/ Microorganisms; Volume 8; Issue 2; Pages: 279 Antarctica polyketides (PKs) non-ribosomal peptides (NRPs) biosynthetic genes computational bioprospection ketosynthase (KS) adenylation (AD) natural products (NPs) Text 2020 ftmdpi https://doi.org/10.3390/microorganisms8020279 2023-07-31T23:07:50Z Bacterial natural products (NPs) are still a major source of new drug leads. Polyketides (PKs) and non-ribosomal peptides (NRP) are two pharmaceutically important families of NPs and recent studies have revealed Antarctica to harbor endemic polyketide synthase (PKS) and non-ribosomal peptide synthetase (NRPS) genes, likely to be involved in the production of novel metabolites. Despite this, the diversity of secondary metabolites genes in Antarctica is still poorly explored. In this study, a computational bioprospection approach was employed to study the diversity and identity of PKS and NRPS genes to one of the most biodiverse areas in maritime Antarctica—Maxwell Bay. Amplicon sequencing of soil samples targeting ketosynthase (KS) and adenylation (AD) domains of PKS and NRPS genes, respectively, revealed abundant and unexplored chemical diversity in this peninsula. About 20% of AD domain sequences were only distantly related to characterized biosynthetic genes. Several PKS and NRPS genes were found to be closely associated to recently described metabolites including those from uncultured and candidate phyla. The combination of new approaches in computational biology and new culture-dependent and -independent strategies is thus critical for the recovery of the potential novel chemistry encoded in Antarctica microorganisms. Text Antarc* Antarctica Maxwell Bay MDPI Open Access Publishing Maxwell Bay ENVELOPE(-58.859,-58.859,-62.223,-62.223) Microorganisms 8 2 279 |
institution |
Open Polar |
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MDPI Open Access Publishing |
op_collection_id |
ftmdpi |
language |
English |
topic |
Antarctica polyketides (PKs) non-ribosomal peptides (NRPs) biosynthetic genes computational bioprospection ketosynthase (KS) adenylation (AD) natural products (NPs) |
spellingShingle |
Antarctica polyketides (PKs) non-ribosomal peptides (NRPs) biosynthetic genes computational bioprospection ketosynthase (KS) adenylation (AD) natural products (NPs) Adriana Rego António G. G. Sousa João P. Santos Francisco Pascoal João Canário Pedro N. Leão Catarina Magalhães Diversity of Bacterial Biosynthetic Genes in Maritime Antarctica |
topic_facet |
Antarctica polyketides (PKs) non-ribosomal peptides (NRPs) biosynthetic genes computational bioprospection ketosynthase (KS) adenylation (AD) natural products (NPs) |
description |
Bacterial natural products (NPs) are still a major source of new drug leads. Polyketides (PKs) and non-ribosomal peptides (NRP) are two pharmaceutically important families of NPs and recent studies have revealed Antarctica to harbor endemic polyketide synthase (PKS) and non-ribosomal peptide synthetase (NRPS) genes, likely to be involved in the production of novel metabolites. Despite this, the diversity of secondary metabolites genes in Antarctica is still poorly explored. In this study, a computational bioprospection approach was employed to study the diversity and identity of PKS and NRPS genes to one of the most biodiverse areas in maritime Antarctica—Maxwell Bay. Amplicon sequencing of soil samples targeting ketosynthase (KS) and adenylation (AD) domains of PKS and NRPS genes, respectively, revealed abundant and unexplored chemical diversity in this peninsula. About 20% of AD domain sequences were only distantly related to characterized biosynthetic genes. Several PKS and NRPS genes were found to be closely associated to recently described metabolites including those from uncultured and candidate phyla. The combination of new approaches in computational biology and new culture-dependent and -independent strategies is thus critical for the recovery of the potential novel chemistry encoded in Antarctica microorganisms. |
format |
Text |
author |
Adriana Rego António G. G. Sousa João P. Santos Francisco Pascoal João Canário Pedro N. Leão Catarina Magalhães |
author_facet |
Adriana Rego António G. G. Sousa João P. Santos Francisco Pascoal João Canário Pedro N. Leão Catarina Magalhães |
author_sort |
Adriana Rego |
title |
Diversity of Bacterial Biosynthetic Genes in Maritime Antarctica |
title_short |
Diversity of Bacterial Biosynthetic Genes in Maritime Antarctica |
title_full |
Diversity of Bacterial Biosynthetic Genes in Maritime Antarctica |
title_fullStr |
Diversity of Bacterial Biosynthetic Genes in Maritime Antarctica |
title_full_unstemmed |
Diversity of Bacterial Biosynthetic Genes in Maritime Antarctica |
title_sort |
diversity of bacterial biosynthetic genes in maritime antarctica |
publisher |
Multidisciplinary Digital Publishing Institute |
publishDate |
2020 |
url |
https://doi.org/10.3390/microorganisms8020279 |
op_coverage |
agris |
long_lat |
ENVELOPE(-58.859,-58.859,-62.223,-62.223) |
geographic |
Maxwell Bay |
geographic_facet |
Maxwell Bay |
genre |
Antarc* Antarctica Maxwell Bay |
genre_facet |
Antarc* Antarctica Maxwell Bay |
op_source |
Microorganisms; Volume 8; Issue 2; Pages: 279 |
op_relation |
Environmental Microbiology https://dx.doi.org/10.3390/microorganisms8020279 |
op_rights |
https://creativecommons.org/licenses/by/4.0/ |
op_doi |
https://doi.org/10.3390/microorganisms8020279 |
container_title |
Microorganisms |
container_volume |
8 |
container_issue |
2 |
container_start_page |
279 |
_version_ |
1774723740120121344 |