Hydrocarbon-Degrading Bacteria Found Tightly Associated with the 50–70 μm Cell-Size Population of Eukaryotic Phytoplankton in Surface Waters of a Northeast Atlantic Region
The surface of marine eukaryotic phytoplankton can harbour communities of hydrocarbon-degrading bacteria; however, this algal–bacterial association has, hitherto, been only examined with non-axenic laboratory cultures of micro-algae. In this study, we isolated an operationally-defined community of p...
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ftmdpi:oai:mdpi.com:/2076-2607/8/12/1955/ 2023-08-20T04:08:40+02:00 Hydrocarbon-Degrading Bacteria Found Tightly Associated with the 50–70 μm Cell-Size Population of Eukaryotic Phytoplankton in Surface Waters of a Northeast Atlantic Region Haydn Frank Thompson Stephen Summers Raif Yuecel Tony Gutierrez agris 2020-12-09 application/pdf https://doi.org/10.3390/microorganisms8121955 EN eng Multidisciplinary Digital Publishing Institute Environmental Microbiology https://dx.doi.org/10.3390/microorganisms8121955 https://creativecommons.org/licenses/by/4.0/ Microorganisms; Volume 8; Issue 12; Pages: 1955 hydrocarbonoclastic bacteria Marinobacter eukaryotic phytoplankton micro-algae phycosphere fluorescence in situ hybridisation (FISH) flow cytometry hydrocarbons marine environment Text 2020 ftmdpi https://doi.org/10.3390/microorganisms8121955 2023-08-01T00:37:53Z The surface of marine eukaryotic phytoplankton can harbour communities of hydrocarbon-degrading bacteria; however, this algal–bacterial association has, hitherto, been only examined with non-axenic laboratory cultures of micro-algae. In this study, we isolated an operationally-defined community of phytoplankton, of cell size 50–70 μm, from a natural community in sea surface waters of a subarctic region in the northeast Atlantic. Using MiSeq 16S rRNA sequencing, we identified several recognized (Alcanivorax, Marinobacter, Oleispira, Porticoccus, Thalassospira) and putative hydrocarbon degraders (Colwelliaceae, Vibrionaceae) tightly associated with the phytoplankton population. We combined fluorescence in situ hybridisation with flow-cytometry (FISH-Flow) to examine the association of Marinobacter with this natural eukaryotic phytoplankton population. About 1.5% of the phytoplankton population contained tightly associated Marinobacter. The remaining Marinobacter population were loosely associated with either eukaryotic phytoplankton cells or non-chlorophyll particulate material. This work is the first to show the presence of obligate, generalist and putative hydrocarbonoclastic bacteria associated with natural populations of eukaryotic phytoplankton directly from sea surface water samples. It also highlights the suitability of FISH-Flow for future studies to examine the spatial and temporal structure and dynamics of these and other algal–bacterial associations in natural seawater samples. Text Northeast Atlantic Subarctic MDPI Open Access Publishing Microorganisms 8 12 1955 |
institution |
Open Polar |
collection |
MDPI Open Access Publishing |
op_collection_id |
ftmdpi |
language |
English |
topic |
hydrocarbonoclastic bacteria Marinobacter eukaryotic phytoplankton micro-algae phycosphere fluorescence in situ hybridisation (FISH) flow cytometry hydrocarbons marine environment |
spellingShingle |
hydrocarbonoclastic bacteria Marinobacter eukaryotic phytoplankton micro-algae phycosphere fluorescence in situ hybridisation (FISH) flow cytometry hydrocarbons marine environment Haydn Frank Thompson Stephen Summers Raif Yuecel Tony Gutierrez Hydrocarbon-Degrading Bacteria Found Tightly Associated with the 50–70 μm Cell-Size Population of Eukaryotic Phytoplankton in Surface Waters of a Northeast Atlantic Region |
topic_facet |
hydrocarbonoclastic bacteria Marinobacter eukaryotic phytoplankton micro-algae phycosphere fluorescence in situ hybridisation (FISH) flow cytometry hydrocarbons marine environment |
description |
The surface of marine eukaryotic phytoplankton can harbour communities of hydrocarbon-degrading bacteria; however, this algal–bacterial association has, hitherto, been only examined with non-axenic laboratory cultures of micro-algae. In this study, we isolated an operationally-defined community of phytoplankton, of cell size 50–70 μm, from a natural community in sea surface waters of a subarctic region in the northeast Atlantic. Using MiSeq 16S rRNA sequencing, we identified several recognized (Alcanivorax, Marinobacter, Oleispira, Porticoccus, Thalassospira) and putative hydrocarbon degraders (Colwelliaceae, Vibrionaceae) tightly associated with the phytoplankton population. We combined fluorescence in situ hybridisation with flow-cytometry (FISH-Flow) to examine the association of Marinobacter with this natural eukaryotic phytoplankton population. About 1.5% of the phytoplankton population contained tightly associated Marinobacter. The remaining Marinobacter population were loosely associated with either eukaryotic phytoplankton cells or non-chlorophyll particulate material. This work is the first to show the presence of obligate, generalist and putative hydrocarbonoclastic bacteria associated with natural populations of eukaryotic phytoplankton directly from sea surface water samples. It also highlights the suitability of FISH-Flow for future studies to examine the spatial and temporal structure and dynamics of these and other algal–bacterial associations in natural seawater samples. |
format |
Text |
author |
Haydn Frank Thompson Stephen Summers Raif Yuecel Tony Gutierrez |
author_facet |
Haydn Frank Thompson Stephen Summers Raif Yuecel Tony Gutierrez |
author_sort |
Haydn Frank Thompson |
title |
Hydrocarbon-Degrading Bacteria Found Tightly Associated with the 50–70 μm Cell-Size Population of Eukaryotic Phytoplankton in Surface Waters of a Northeast Atlantic Region |
title_short |
Hydrocarbon-Degrading Bacteria Found Tightly Associated with the 50–70 μm Cell-Size Population of Eukaryotic Phytoplankton in Surface Waters of a Northeast Atlantic Region |
title_full |
Hydrocarbon-Degrading Bacteria Found Tightly Associated with the 50–70 μm Cell-Size Population of Eukaryotic Phytoplankton in Surface Waters of a Northeast Atlantic Region |
title_fullStr |
Hydrocarbon-Degrading Bacteria Found Tightly Associated with the 50–70 μm Cell-Size Population of Eukaryotic Phytoplankton in Surface Waters of a Northeast Atlantic Region |
title_full_unstemmed |
Hydrocarbon-Degrading Bacteria Found Tightly Associated with the 50–70 μm Cell-Size Population of Eukaryotic Phytoplankton in Surface Waters of a Northeast Atlantic Region |
title_sort |
hydrocarbon-degrading bacteria found tightly associated with the 50–70 μm cell-size population of eukaryotic phytoplankton in surface waters of a northeast atlantic region |
publisher |
Multidisciplinary Digital Publishing Institute |
publishDate |
2020 |
url |
https://doi.org/10.3390/microorganisms8121955 |
op_coverage |
agris |
genre |
Northeast Atlantic Subarctic |
genre_facet |
Northeast Atlantic Subarctic |
op_source |
Microorganisms; Volume 8; Issue 12; Pages: 1955 |
op_relation |
Environmental Microbiology https://dx.doi.org/10.3390/microorganisms8121955 |
op_rights |
https://creativecommons.org/licenses/by/4.0/ |
op_doi |
https://doi.org/10.3390/microorganisms8121955 |
container_title |
Microorganisms |
container_volume |
8 |
container_issue |
12 |
container_start_page |
1955 |
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1774721071598010368 |