Comparative Genomics Analysis of Vibrio anguillarum Isolated from Lumpfish (Cyclopterus lumpus) in Newfoundland Reveal Novel Chromosomal Organizations
Vibrio anguillarum is a Gram-negative marine pathogen causative agent of vibriosis in a wide range of hosts, including invertebrates and teleosts. Lumpfish (Cyclopterus lumpus), a native fish of the North Atlantic Ocean, is utilized as cleaner fish to control sea lice (Lepeophtheirus salmonis) infes...
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ftmdpi:oai:mdpi.com:/2076-2607/8/11/1666/ 2023-08-20T04:05:20+02:00 Comparative Genomics Analysis of Vibrio anguillarum Isolated from Lumpfish (Cyclopterus lumpus) in Newfoundland Reveal Novel Chromosomal Organizations Ignacio Vasquez Trung Cao Setu Chakraborty Hajarooba Gnanagobal Nicole O’Brien Jennifer Monk Danny Boyce Jillian D. Westcott Javier Santander agris 2020-10-27 application/pdf https://doi.org/10.3390/microorganisms8111666 EN eng Multidisciplinary Digital Publishing Institute Environmental Microbiology https://dx.doi.org/10.3390/microorganisms8111666 https://creativecommons.org/licenses/by/4.0/ Microorganisms; Volume 8; Issue 11; Pages: 1666 Vibrio anguillarum lumpfish genomics insertion elements evolution Text 2020 ftmdpi https://doi.org/10.3390/microorganisms8111666 2023-08-01T00:21:32Z Vibrio anguillarum is a Gram-negative marine pathogen causative agent of vibriosis in a wide range of hosts, including invertebrates and teleosts. Lumpfish (Cyclopterus lumpus), a native fish of the North Atlantic Ocean, is utilized as cleaner fish to control sea lice (Lepeophtheirus salmonis) infestations in the Atlantic salmon (Salmo salar) aquaculture industry. V. anguillarum is one of the most frequent bacterial pathogens affecting lumpfish. Here, we described the phenotype and genomic characteristics of V. anguillarum strain J360 isolated from infected cultured lumpfish in Newfoundland, Canada. Koch’s postulates determined in naïve lumpfish showed lethal acute vibriosis in lumpfish. The V. anguillarum J360 genome was shown to be composed of two chromosomes and two plasmids with a total genome size of 4.56 Mb with 44.85% G + C content. Phylogenetic and comparative analyses showed that V. anguillarum J360 is closely related to V. anguillarum strain VIB43, isolated in Scotland, with a 99.8% genome identity. Differences in the genomic organization were identified and associated with insertion sequence elements (ISs). Additionally, V. anguillarum J360 does not possess a pJM1-like plasmid, typically present in virulent isolates from the Pacific Ocean, suggesting that acquisition of this extrachromosomal element and the virulence of V. anguillarum J360 or other Atlantic isolates could increase. Text Atlantic salmon Newfoundland North Atlantic Salmo salar MDPI Open Access Publishing Canada Pacific Microorganisms 8 11 1666 |
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Open Polar |
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MDPI Open Access Publishing |
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ftmdpi |
language |
English |
topic |
Vibrio anguillarum lumpfish genomics insertion elements evolution |
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Vibrio anguillarum lumpfish genomics insertion elements evolution Ignacio Vasquez Trung Cao Setu Chakraborty Hajarooba Gnanagobal Nicole O’Brien Jennifer Monk Danny Boyce Jillian D. Westcott Javier Santander Comparative Genomics Analysis of Vibrio anguillarum Isolated from Lumpfish (Cyclopterus lumpus) in Newfoundland Reveal Novel Chromosomal Organizations |
topic_facet |
Vibrio anguillarum lumpfish genomics insertion elements evolution |
description |
Vibrio anguillarum is a Gram-negative marine pathogen causative agent of vibriosis in a wide range of hosts, including invertebrates and teleosts. Lumpfish (Cyclopterus lumpus), a native fish of the North Atlantic Ocean, is utilized as cleaner fish to control sea lice (Lepeophtheirus salmonis) infestations in the Atlantic salmon (Salmo salar) aquaculture industry. V. anguillarum is one of the most frequent bacterial pathogens affecting lumpfish. Here, we described the phenotype and genomic characteristics of V. anguillarum strain J360 isolated from infected cultured lumpfish in Newfoundland, Canada. Koch’s postulates determined in naïve lumpfish showed lethal acute vibriosis in lumpfish. The V. anguillarum J360 genome was shown to be composed of two chromosomes and two plasmids with a total genome size of 4.56 Mb with 44.85% G + C content. Phylogenetic and comparative analyses showed that V. anguillarum J360 is closely related to V. anguillarum strain VIB43, isolated in Scotland, with a 99.8% genome identity. Differences in the genomic organization were identified and associated with insertion sequence elements (ISs). Additionally, V. anguillarum J360 does not possess a pJM1-like plasmid, typically present in virulent isolates from the Pacific Ocean, suggesting that acquisition of this extrachromosomal element and the virulence of V. anguillarum J360 or other Atlantic isolates could increase. |
format |
Text |
author |
Ignacio Vasquez Trung Cao Setu Chakraborty Hajarooba Gnanagobal Nicole O’Brien Jennifer Monk Danny Boyce Jillian D. Westcott Javier Santander |
author_facet |
Ignacio Vasquez Trung Cao Setu Chakraborty Hajarooba Gnanagobal Nicole O’Brien Jennifer Monk Danny Boyce Jillian D. Westcott Javier Santander |
author_sort |
Ignacio Vasquez |
title |
Comparative Genomics Analysis of Vibrio anguillarum Isolated from Lumpfish (Cyclopterus lumpus) in Newfoundland Reveal Novel Chromosomal Organizations |
title_short |
Comparative Genomics Analysis of Vibrio anguillarum Isolated from Lumpfish (Cyclopterus lumpus) in Newfoundland Reveal Novel Chromosomal Organizations |
title_full |
Comparative Genomics Analysis of Vibrio anguillarum Isolated from Lumpfish (Cyclopterus lumpus) in Newfoundland Reveal Novel Chromosomal Organizations |
title_fullStr |
Comparative Genomics Analysis of Vibrio anguillarum Isolated from Lumpfish (Cyclopterus lumpus) in Newfoundland Reveal Novel Chromosomal Organizations |
title_full_unstemmed |
Comparative Genomics Analysis of Vibrio anguillarum Isolated from Lumpfish (Cyclopterus lumpus) in Newfoundland Reveal Novel Chromosomal Organizations |
title_sort |
comparative genomics analysis of vibrio anguillarum isolated from lumpfish (cyclopterus lumpus) in newfoundland reveal novel chromosomal organizations |
publisher |
Multidisciplinary Digital Publishing Institute |
publishDate |
2020 |
url |
https://doi.org/10.3390/microorganisms8111666 |
op_coverage |
agris |
geographic |
Canada Pacific |
geographic_facet |
Canada Pacific |
genre |
Atlantic salmon Newfoundland North Atlantic Salmo salar |
genre_facet |
Atlantic salmon Newfoundland North Atlantic Salmo salar |
op_source |
Microorganisms; Volume 8; Issue 11; Pages: 1666 |
op_relation |
Environmental Microbiology https://dx.doi.org/10.3390/microorganisms8111666 |
op_rights |
https://creativecommons.org/licenses/by/4.0/ |
op_doi |
https://doi.org/10.3390/microorganisms8111666 |
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Microorganisms |
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8 |
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11 |
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1666 |
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1774715825504124928 |