Cold-Active, Heterotrophic Bacteria from the Highly Oligotrophic Waters of Lake Vanda, Antarctica

The permanently ice-covered lakes of the McMurdo Dry Valleys, Antarctica are distinctive ecosystems that consist strictly of microbial communities. In this study, water samples were collected from Lake Vanda, a stratified Dry Valley lake whose upper waters (from just below the ice cover to nearly 60...

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Published in:Microorganisms
Main Authors: Nicole Vander Schaaf, Anna Cunningham, Brandon Cluff, CodyJo Kraemer, Chelsea Reeves, Carli Riester, Lauren Slater, Michael Madigan, W. Sattley
Format: Text
Language:English
Published: Multidisciplinary Digital Publishing Institute 2015
Subjects:
Online Access:https://doi.org/10.3390/microorganisms3030391
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spelling ftmdpi:oai:mdpi.com:/2076-2607/3/3/391/ 2023-08-20T04:01:36+02:00 Cold-Active, Heterotrophic Bacteria from the Highly Oligotrophic Waters of Lake Vanda, Antarctica Nicole Vander Schaaf Anna Cunningham Brandon Cluff CodyJo Kraemer Chelsea Reeves Carli Riester Lauren Slater Michael Madigan W. Sattley agris 2015-07-24 application/pdf https://doi.org/10.3390/microorganisms3030391 EN eng Multidisciplinary Digital Publishing Institute Environmental Microbiology https://dx.doi.org/10.3390/microorganisms3030391 https://creativecommons.org/licenses/by/4.0/ Microorganisms; Volume 3; Issue 3; Pages: 391-406 Antarctica Lake Vanda McMurdo Dry Valleys psychrotolerant halotolerant Text 2015 ftmdpi https://doi.org/10.3390/microorganisms3030391 2023-07-31T20:45:19Z The permanently ice-covered lakes of the McMurdo Dry Valleys, Antarctica are distinctive ecosystems that consist strictly of microbial communities. In this study, water samples were collected from Lake Vanda, a stratified Dry Valley lake whose upper waters (from just below the ice cover to nearly 60 m) are highly oligotrophic, and used to establish enrichment cultures. Six strains of psychrotolerant, heterotrophic bacteria were isolated from lake water samples from a depth of 50 or 55 m. Phylogenetic analyses showed the Lake Vanda strains to be species of Nocardiaceae, Caulobacteraceae, Sphingomonadaceae, and Bradyrhizobiaceae. All Lake Vanda strains grew at temperatures near or below 0 °C, but optimal growth occurred from 18 to 24 °C. Some strains showed significant halotolerance, but no strains required NaCl for growth. The isolates described herein include cold-active species not previously reported from Dry Valley lakes, and their physiological and phylogenetic characterization broadens our understanding of these limnologically unique lakes. Text Antarc* Antarctica McMurdo Dry Valleys MDPI Open Access Publishing McMurdo Dry Valleys Vanda ENVELOPE(161.550,161.550,-77.533,-77.533) Lake Vanda ENVELOPE(161.600,161.600,-77.517,-77.517) Microorganisms 3 3 391 406
institution Open Polar
collection MDPI Open Access Publishing
op_collection_id ftmdpi
language English
topic Antarctica
Lake Vanda
McMurdo Dry Valleys
psychrotolerant
halotolerant
spellingShingle Antarctica
Lake Vanda
McMurdo Dry Valleys
psychrotolerant
halotolerant
Nicole Vander Schaaf
Anna Cunningham
Brandon Cluff
CodyJo Kraemer
Chelsea Reeves
Carli Riester
Lauren Slater
Michael Madigan
W. Sattley
Cold-Active, Heterotrophic Bacteria from the Highly Oligotrophic Waters of Lake Vanda, Antarctica
topic_facet Antarctica
Lake Vanda
McMurdo Dry Valleys
psychrotolerant
halotolerant
description The permanently ice-covered lakes of the McMurdo Dry Valleys, Antarctica are distinctive ecosystems that consist strictly of microbial communities. In this study, water samples were collected from Lake Vanda, a stratified Dry Valley lake whose upper waters (from just below the ice cover to nearly 60 m) are highly oligotrophic, and used to establish enrichment cultures. Six strains of psychrotolerant, heterotrophic bacteria were isolated from lake water samples from a depth of 50 or 55 m. Phylogenetic analyses showed the Lake Vanda strains to be species of Nocardiaceae, Caulobacteraceae, Sphingomonadaceae, and Bradyrhizobiaceae. All Lake Vanda strains grew at temperatures near or below 0 °C, but optimal growth occurred from 18 to 24 °C. Some strains showed significant halotolerance, but no strains required NaCl for growth. The isolates described herein include cold-active species not previously reported from Dry Valley lakes, and their physiological and phylogenetic characterization broadens our understanding of these limnologically unique lakes.
format Text
author Nicole Vander Schaaf
Anna Cunningham
Brandon Cluff
CodyJo Kraemer
Chelsea Reeves
Carli Riester
Lauren Slater
Michael Madigan
W. Sattley
author_facet Nicole Vander Schaaf
Anna Cunningham
Brandon Cluff
CodyJo Kraemer
Chelsea Reeves
Carli Riester
Lauren Slater
Michael Madigan
W. Sattley
author_sort Nicole Vander Schaaf
title Cold-Active, Heterotrophic Bacteria from the Highly Oligotrophic Waters of Lake Vanda, Antarctica
title_short Cold-Active, Heterotrophic Bacteria from the Highly Oligotrophic Waters of Lake Vanda, Antarctica
title_full Cold-Active, Heterotrophic Bacteria from the Highly Oligotrophic Waters of Lake Vanda, Antarctica
title_fullStr Cold-Active, Heterotrophic Bacteria from the Highly Oligotrophic Waters of Lake Vanda, Antarctica
title_full_unstemmed Cold-Active, Heterotrophic Bacteria from the Highly Oligotrophic Waters of Lake Vanda, Antarctica
title_sort cold-active, heterotrophic bacteria from the highly oligotrophic waters of lake vanda, antarctica
publisher Multidisciplinary Digital Publishing Institute
publishDate 2015
url https://doi.org/10.3390/microorganisms3030391
op_coverage agris
long_lat ENVELOPE(161.550,161.550,-77.533,-77.533)
ENVELOPE(161.600,161.600,-77.517,-77.517)
geographic McMurdo Dry Valleys
Vanda
Lake Vanda
geographic_facet McMurdo Dry Valleys
Vanda
Lake Vanda
genre Antarc*
Antarctica
McMurdo Dry Valleys
genre_facet Antarc*
Antarctica
McMurdo Dry Valleys
op_source Microorganisms; Volume 3; Issue 3; Pages: 391-406
op_relation Environmental Microbiology
https://dx.doi.org/10.3390/microorganisms3030391
op_rights https://creativecommons.org/licenses/by/4.0/
op_doi https://doi.org/10.3390/microorganisms3030391
container_title Microorganisms
container_volume 3
container_issue 3
container_start_page 391
op_container_end_page 406
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