Metagenomic Analysis of Antarctic Biocrusts Unveils a Rich Range of Cold-Shock Proteins
Microorganisms inhabiting Antarctic biocrusts develop several strategies to survive extreme environmental conditions such as severe cold and drought. However, the knowledge about adaptations of biocrusts microorganisms are limited. Here, we applied metagenomic sequencing to study biocrusts from east...
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Online Access: | https://doi.org/10.3390/microorganisms11081932 |
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ftmdpi:oai:mdpi.com:/2076-2607/11/8/1932/ 2023-08-20T04:02:25+02:00 Metagenomic Analysis of Antarctic Biocrusts Unveils a Rich Range of Cold-Shock Proteins Ekaterina Pushkareva Josef Elster Burkhard Becker agris 2023-07-28 application/pdf https://doi.org/10.3390/microorganisms11081932 EN eng Multidisciplinary Digital Publishing Institute Environmental Microbiology https://dx.doi.org/10.3390/microorganisms11081932 https://creativecommons.org/licenses/by/4.0/ Microorganisms; Volume 11; Issue 8; Pages: 1932 metagenomic sequencing biocrusts Antarctica cold-shock proteins antifreeze proteins cold-shock domain Text 2023 ftmdpi https://doi.org/10.3390/microorganisms11081932 2023-08-01T11:03:59Z Microorganisms inhabiting Antarctic biocrusts develop several strategies to survive extreme environmental conditions such as severe cold and drought. However, the knowledge about adaptations of biocrusts microorganisms are limited. Here, we applied metagenomic sequencing to study biocrusts from east Antarctica. Biocrusts were dominated by cyanobacteria, actinobacteria and proteobacteria. Furthermore, the results provided insights into the presence and abundance of cold shock proteins (Csp), cold shock domain A proteins (CsdA), and antifreeze proteins (AFP) in these extreme environments. The metagenomic analysis revealed a high number of CsdA across the samples. The majority of the Csp recorded in the studied biocrusts were Csp A, C, and E. In addition, CsdA, Csp, and AFP primarily originated from proteobacteria and actinobacteria. Text Antarc* Antarctic Antarctica East Antarctica MDPI Open Access Publishing Antarctic East Antarctica Microorganisms 11 8 1932 |
institution |
Open Polar |
collection |
MDPI Open Access Publishing |
op_collection_id |
ftmdpi |
language |
English |
topic |
metagenomic sequencing biocrusts Antarctica cold-shock proteins antifreeze proteins cold-shock domain |
spellingShingle |
metagenomic sequencing biocrusts Antarctica cold-shock proteins antifreeze proteins cold-shock domain Ekaterina Pushkareva Josef Elster Burkhard Becker Metagenomic Analysis of Antarctic Biocrusts Unveils a Rich Range of Cold-Shock Proteins |
topic_facet |
metagenomic sequencing biocrusts Antarctica cold-shock proteins antifreeze proteins cold-shock domain |
description |
Microorganisms inhabiting Antarctic biocrusts develop several strategies to survive extreme environmental conditions such as severe cold and drought. However, the knowledge about adaptations of biocrusts microorganisms are limited. Here, we applied metagenomic sequencing to study biocrusts from east Antarctica. Biocrusts were dominated by cyanobacteria, actinobacteria and proteobacteria. Furthermore, the results provided insights into the presence and abundance of cold shock proteins (Csp), cold shock domain A proteins (CsdA), and antifreeze proteins (AFP) in these extreme environments. The metagenomic analysis revealed a high number of CsdA across the samples. The majority of the Csp recorded in the studied biocrusts were Csp A, C, and E. In addition, CsdA, Csp, and AFP primarily originated from proteobacteria and actinobacteria. |
format |
Text |
author |
Ekaterina Pushkareva Josef Elster Burkhard Becker |
author_facet |
Ekaterina Pushkareva Josef Elster Burkhard Becker |
author_sort |
Ekaterina Pushkareva |
title |
Metagenomic Analysis of Antarctic Biocrusts Unveils a Rich Range of Cold-Shock Proteins |
title_short |
Metagenomic Analysis of Antarctic Biocrusts Unveils a Rich Range of Cold-Shock Proteins |
title_full |
Metagenomic Analysis of Antarctic Biocrusts Unveils a Rich Range of Cold-Shock Proteins |
title_fullStr |
Metagenomic Analysis of Antarctic Biocrusts Unveils a Rich Range of Cold-Shock Proteins |
title_full_unstemmed |
Metagenomic Analysis of Antarctic Biocrusts Unveils a Rich Range of Cold-Shock Proteins |
title_sort |
metagenomic analysis of antarctic biocrusts unveils a rich range of cold-shock proteins |
publisher |
Multidisciplinary Digital Publishing Institute |
publishDate |
2023 |
url |
https://doi.org/10.3390/microorganisms11081932 |
op_coverage |
agris |
geographic |
Antarctic East Antarctica |
geographic_facet |
Antarctic East Antarctica |
genre |
Antarc* Antarctic Antarctica East Antarctica |
genre_facet |
Antarc* Antarctic Antarctica East Antarctica |
op_source |
Microorganisms; Volume 11; Issue 8; Pages: 1932 |
op_relation |
Environmental Microbiology https://dx.doi.org/10.3390/microorganisms11081932 |
op_rights |
https://creativecommons.org/licenses/by/4.0/ |
op_doi |
https://doi.org/10.3390/microorganisms11081932 |
container_title |
Microorganisms |
container_volume |
11 |
container_issue |
8 |
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1932 |
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1774712865278656512 |