Comparative Genomic Analysis of a Novel Vibrio sp. Isolated from an Ulcer Disease Event in Atlantic Salmon (Salmo salar)
Ulcer diseases are a recalcitrant issue at Atlantic salmon (Salmo salar) aquaculture cage-sites across the North Atlantic region. Classical ulcerative outbreaks (also called winter ulcer disease) refer to a skin infection caused by Moritella viscosa. However, several bacterial species are frequently...
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ftmdpi:oai:mdpi.com:/2076-2607/11/7/1736/ 2023-08-20T04:05:13+02:00 Comparative Genomic Analysis of a Novel Vibrio sp. Isolated from an Ulcer Disease Event in Atlantic Salmon (Salmo salar) Maryam Ghasemieshkaftaki Ignacio Vasquez Aria Eshraghi Anthony Kurt Gamperl Javier Santander agris 2023-07-02 application/pdf https://doi.org/10.3390/microorganisms11071736 EN eng Multidisciplinary Digital Publishing Institute Veterinary Microbiology https://dx.doi.org/10.3390/microorganisms11071736 https://creativecommons.org/licenses/by/4.0/ Microorganisms; Volume 11; Issue 7; Pages: 1736 Atlantic salmon Vibrio sp. J383 genomics phylogenetics phenotype ulcer disease Text 2023 ftmdpi https://doi.org/10.3390/microorganisms11071736 2023-08-01T10:43:02Z Ulcer diseases are a recalcitrant issue at Atlantic salmon (Salmo salar) aquaculture cage-sites across the North Atlantic region. Classical ulcerative outbreaks (also called winter ulcer disease) refer to a skin infection caused by Moritella viscosa. However, several bacterial species are frequently isolated from ulcer disease events, and it is unclear if other undescribed pathogens are implicated in ulcer disease in Atlantic salmon. Although different polyvalent vaccines are used against M. viscosa, ulcerative outbreaks are continuously reported in Atlantic salmon in Canada. This study analyzed the phenotypical and genomic characteristics of Vibrio sp. J383 isolated from internal organs of vaccinated farmed Atlantic salmon displaying clinical signs of ulcer disease. Infection assays conducted on vaccinated farmed Atlantic salmon and revealed that Vibrio sp. J383 causes a low level of mortalities when administered intracelomic at doses ranging from 107–108 CFU/dose. Vibrio sp. J383 persisted in the blood of infected fish for at least 8 weeks at 10 and 12 °C. Clinical signs of this disease were greatest 12 °C, but no mortality and bacteremia were observed at 16 °C. The Vibrio sp. J383 genome (5,902,734 bp) has two chromosomes of 3,633,265 bp and 2,068,312 bp, respectively, and one large plasmid of 201,166 bp. Phylogenetic and comparative analyses indicated that Vibrio sp. J383 is related to V. splendidus, with 93% identity. Furthermore, the phenotypic analysis showed that there were significant differences between Vibrio sp. J383 and other Vibrio spp, suggesting J383 is a novel Vibrio species adapted to cold temperatures. Text Atlantic salmon North Atlantic Salmo salar MDPI Open Access Publishing Canada Microorganisms 11 7 1736 |
institution |
Open Polar |
collection |
MDPI Open Access Publishing |
op_collection_id |
ftmdpi |
language |
English |
topic |
Atlantic salmon Vibrio sp. J383 genomics phylogenetics phenotype ulcer disease |
spellingShingle |
Atlantic salmon Vibrio sp. J383 genomics phylogenetics phenotype ulcer disease Maryam Ghasemieshkaftaki Ignacio Vasquez Aria Eshraghi Anthony Kurt Gamperl Javier Santander Comparative Genomic Analysis of a Novel Vibrio sp. Isolated from an Ulcer Disease Event in Atlantic Salmon (Salmo salar) |
topic_facet |
Atlantic salmon Vibrio sp. J383 genomics phylogenetics phenotype ulcer disease |
description |
Ulcer diseases are a recalcitrant issue at Atlantic salmon (Salmo salar) aquaculture cage-sites across the North Atlantic region. Classical ulcerative outbreaks (also called winter ulcer disease) refer to a skin infection caused by Moritella viscosa. However, several bacterial species are frequently isolated from ulcer disease events, and it is unclear if other undescribed pathogens are implicated in ulcer disease in Atlantic salmon. Although different polyvalent vaccines are used against M. viscosa, ulcerative outbreaks are continuously reported in Atlantic salmon in Canada. This study analyzed the phenotypical and genomic characteristics of Vibrio sp. J383 isolated from internal organs of vaccinated farmed Atlantic salmon displaying clinical signs of ulcer disease. Infection assays conducted on vaccinated farmed Atlantic salmon and revealed that Vibrio sp. J383 causes a low level of mortalities when administered intracelomic at doses ranging from 107–108 CFU/dose. Vibrio sp. J383 persisted in the blood of infected fish for at least 8 weeks at 10 and 12 °C. Clinical signs of this disease were greatest 12 °C, but no mortality and bacteremia were observed at 16 °C. The Vibrio sp. J383 genome (5,902,734 bp) has two chromosomes of 3,633,265 bp and 2,068,312 bp, respectively, and one large plasmid of 201,166 bp. Phylogenetic and comparative analyses indicated that Vibrio sp. J383 is related to V. splendidus, with 93% identity. Furthermore, the phenotypic analysis showed that there were significant differences between Vibrio sp. J383 and other Vibrio spp, suggesting J383 is a novel Vibrio species adapted to cold temperatures. |
format |
Text |
author |
Maryam Ghasemieshkaftaki Ignacio Vasquez Aria Eshraghi Anthony Kurt Gamperl Javier Santander |
author_facet |
Maryam Ghasemieshkaftaki Ignacio Vasquez Aria Eshraghi Anthony Kurt Gamperl Javier Santander |
author_sort |
Maryam Ghasemieshkaftaki |
title |
Comparative Genomic Analysis of a Novel Vibrio sp. Isolated from an Ulcer Disease Event in Atlantic Salmon (Salmo salar) |
title_short |
Comparative Genomic Analysis of a Novel Vibrio sp. Isolated from an Ulcer Disease Event in Atlantic Salmon (Salmo salar) |
title_full |
Comparative Genomic Analysis of a Novel Vibrio sp. Isolated from an Ulcer Disease Event in Atlantic Salmon (Salmo salar) |
title_fullStr |
Comparative Genomic Analysis of a Novel Vibrio sp. Isolated from an Ulcer Disease Event in Atlantic Salmon (Salmo salar) |
title_full_unstemmed |
Comparative Genomic Analysis of a Novel Vibrio sp. Isolated from an Ulcer Disease Event in Atlantic Salmon (Salmo salar) |
title_sort |
comparative genomic analysis of a novel vibrio sp. isolated from an ulcer disease event in atlantic salmon (salmo salar) |
publisher |
Multidisciplinary Digital Publishing Institute |
publishDate |
2023 |
url |
https://doi.org/10.3390/microorganisms11071736 |
op_coverage |
agris |
geographic |
Canada |
geographic_facet |
Canada |
genre |
Atlantic salmon North Atlantic Salmo salar |
genre_facet |
Atlantic salmon North Atlantic Salmo salar |
op_source |
Microorganisms; Volume 11; Issue 7; Pages: 1736 |
op_relation |
Veterinary Microbiology https://dx.doi.org/10.3390/microorganisms11071736 |
op_rights |
https://creativecommons.org/licenses/by/4.0/ |
op_doi |
https://doi.org/10.3390/microorganisms11071736 |
container_title |
Microorganisms |
container_volume |
11 |
container_issue |
7 |
container_start_page |
1736 |
_version_ |
1774715687999111168 |