Unraveling the Genomic Potential of the Thermophilic Bacterium Anoxybacillus flavithermus from an Antarctic Geothermal Environment
Antarctica is a mosaic of extremes. It harbors active polar volcanoes, such as Deception Island, a marine stratovolcano having notable temperature gradients over very short distances, with the temperature reaching up to 100 °C near the fumaroles and subzero temperatures being noted in the glaciers....
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ftmdpi:oai:mdpi.com:/2076-2607/10/8/1673/ 2023-08-20T04:01:52+02:00 Unraveling the Genomic Potential of the Thermophilic Bacterium Anoxybacillus flavithermus from an Antarctic Geothermal Environment Júnia Schultz Mariana Teixeira Dornelles Parise Doglas Parise Laenne G. Medeiros Thiago J. Sousa Rodrigo B. Kato Ana Paula Trovatti Uetanabaro Fabrício Araújo Rommel Thiago Jucá Ramos Siomar de Castro Soares Bertram Brenig Vasco Ariston de Carvalho Azevedo Aristóteles Góes-Neto Alexandre S. Rosado agris 2022-08-19 application/pdf https://doi.org/10.3390/microorganisms10081673 EN eng Multidisciplinary Digital Publishing Institute Environmental Microbiology https://dx.doi.org/10.3390/microorganisms10081673 https://creativecommons.org/licenses/by/4.0/ Microorganisms; Volume 10; Issue 8; Pages: 1673 Anoxybacillus comparative genomics phylogeny potential metabolic functions antarctica polar volcano Text 2022 ftmdpi https://doi.org/10.3390/microorganisms10081673 2023-08-01T06:08:49Z Antarctica is a mosaic of extremes. It harbors active polar volcanoes, such as Deception Island, a marine stratovolcano having notable temperature gradients over very short distances, with the temperature reaching up to 100 °C near the fumaroles and subzero temperatures being noted in the glaciers. From the sediments of Deception Island, we isolated representatives of the genus Anoxybacillus, a widely spread genus that is mainly encountered in thermophilic environments. However, the phylogeny of this genus and its adaptive mechanisms in the geothermal sites of cold environments remain unknown. To the best of our knowledge, this is the first study to unravel the genomic features and provide insights into the phylogenomics and metabolic potential of members of the genus Anoxybacillus inhabiting the Antarctic thermophilic ecosystem. Here, we report the genome sequencing data of seven A. flavithermus strains isolated from two geothermal sites on Deception Island, Antarctic Peninsula. Their genomes were approximately 3.0 Mb in size, had a G + C ratio of 42%, and were predicted to encode 3500 proteins on average. We observed that the strains were phylogenomically closest to each other (Average Nucleotide Identity (ANI) > 98%) and to A. flavithermus (ANI 95%). In silico genomic analysis revealed 15 resistance and metabolic islands, as well as genes related to genome stabilization, DNA repair systems against UV radiation threats, temperature adaptation, heat- and cold-shock proteins (Csps), and resistance to alkaline conditions. Remarkably, glycosyl hydrolase enzyme-encoding genes, secondary metabolites, and prophage sequences were predicted, revealing metabolic and cellular capabilities for potential biotechnological applications. Text Antarc* Antarctic Antarctic Peninsula Antarctica Deception Island MDPI Open Access Publishing Antarctic The Antarctic Antarctic Peninsula Deception Island ENVELOPE(-60.633,-60.633,-62.950,-62.950) Microorganisms 10 8 1673 |
institution |
Open Polar |
collection |
MDPI Open Access Publishing |
op_collection_id |
ftmdpi |
language |
English |
topic |
Anoxybacillus comparative genomics phylogeny potential metabolic functions antarctica polar volcano |
spellingShingle |
Anoxybacillus comparative genomics phylogeny potential metabolic functions antarctica polar volcano Júnia Schultz Mariana Teixeira Dornelles Parise Doglas Parise Laenne G. Medeiros Thiago J. Sousa Rodrigo B. Kato Ana Paula Trovatti Uetanabaro Fabrício Araújo Rommel Thiago Jucá Ramos Siomar de Castro Soares Bertram Brenig Vasco Ariston de Carvalho Azevedo Aristóteles Góes-Neto Alexandre S. Rosado Unraveling the Genomic Potential of the Thermophilic Bacterium Anoxybacillus flavithermus from an Antarctic Geothermal Environment |
topic_facet |
Anoxybacillus comparative genomics phylogeny potential metabolic functions antarctica polar volcano |
description |
Antarctica is a mosaic of extremes. It harbors active polar volcanoes, such as Deception Island, a marine stratovolcano having notable temperature gradients over very short distances, with the temperature reaching up to 100 °C near the fumaroles and subzero temperatures being noted in the glaciers. From the sediments of Deception Island, we isolated representatives of the genus Anoxybacillus, a widely spread genus that is mainly encountered in thermophilic environments. However, the phylogeny of this genus and its adaptive mechanisms in the geothermal sites of cold environments remain unknown. To the best of our knowledge, this is the first study to unravel the genomic features and provide insights into the phylogenomics and metabolic potential of members of the genus Anoxybacillus inhabiting the Antarctic thermophilic ecosystem. Here, we report the genome sequencing data of seven A. flavithermus strains isolated from two geothermal sites on Deception Island, Antarctic Peninsula. Their genomes were approximately 3.0 Mb in size, had a G + C ratio of 42%, and were predicted to encode 3500 proteins on average. We observed that the strains were phylogenomically closest to each other (Average Nucleotide Identity (ANI) > 98%) and to A. flavithermus (ANI 95%). In silico genomic analysis revealed 15 resistance and metabolic islands, as well as genes related to genome stabilization, DNA repair systems against UV radiation threats, temperature adaptation, heat- and cold-shock proteins (Csps), and resistance to alkaline conditions. Remarkably, glycosyl hydrolase enzyme-encoding genes, secondary metabolites, and prophage sequences were predicted, revealing metabolic and cellular capabilities for potential biotechnological applications. |
format |
Text |
author |
Júnia Schultz Mariana Teixeira Dornelles Parise Doglas Parise Laenne G. Medeiros Thiago J. Sousa Rodrigo B. Kato Ana Paula Trovatti Uetanabaro Fabrício Araújo Rommel Thiago Jucá Ramos Siomar de Castro Soares Bertram Brenig Vasco Ariston de Carvalho Azevedo Aristóteles Góes-Neto Alexandre S. Rosado |
author_facet |
Júnia Schultz Mariana Teixeira Dornelles Parise Doglas Parise Laenne G. Medeiros Thiago J. Sousa Rodrigo B. Kato Ana Paula Trovatti Uetanabaro Fabrício Araújo Rommel Thiago Jucá Ramos Siomar de Castro Soares Bertram Brenig Vasco Ariston de Carvalho Azevedo Aristóteles Góes-Neto Alexandre S. Rosado |
author_sort |
Júnia Schultz |
title |
Unraveling the Genomic Potential of the Thermophilic Bacterium Anoxybacillus flavithermus from an Antarctic Geothermal Environment |
title_short |
Unraveling the Genomic Potential of the Thermophilic Bacterium Anoxybacillus flavithermus from an Antarctic Geothermal Environment |
title_full |
Unraveling the Genomic Potential of the Thermophilic Bacterium Anoxybacillus flavithermus from an Antarctic Geothermal Environment |
title_fullStr |
Unraveling the Genomic Potential of the Thermophilic Bacterium Anoxybacillus flavithermus from an Antarctic Geothermal Environment |
title_full_unstemmed |
Unraveling the Genomic Potential of the Thermophilic Bacterium Anoxybacillus flavithermus from an Antarctic Geothermal Environment |
title_sort |
unraveling the genomic potential of the thermophilic bacterium anoxybacillus flavithermus from an antarctic geothermal environment |
publisher |
Multidisciplinary Digital Publishing Institute |
publishDate |
2022 |
url |
https://doi.org/10.3390/microorganisms10081673 |
op_coverage |
agris |
long_lat |
ENVELOPE(-60.633,-60.633,-62.950,-62.950) |
geographic |
Antarctic The Antarctic Antarctic Peninsula Deception Island |
geographic_facet |
Antarctic The Antarctic Antarctic Peninsula Deception Island |
genre |
Antarc* Antarctic Antarctic Peninsula Antarctica Deception Island |
genre_facet |
Antarc* Antarctic Antarctic Peninsula Antarctica Deception Island |
op_source |
Microorganisms; Volume 10; Issue 8; Pages: 1673 |
op_relation |
Environmental Microbiology https://dx.doi.org/10.3390/microorganisms10081673 |
op_rights |
https://creativecommons.org/licenses/by/4.0/ |
op_doi |
https://doi.org/10.3390/microorganisms10081673 |
container_title |
Microorganisms |
container_volume |
10 |
container_issue |
8 |
container_start_page |
1673 |
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1774712240651370496 |