Characterisation of Waterborne Psychrophilic Massilia Isolates with Violacein Production and Description of Massilia antarctica sp. nov.
A group of seven bacterial strains producing blue-purple pigmented colonies on R2A agar was isolated from freshwater samples collected in a deglaciated part of James Ross Island and Eagle Island, Antarctica, from 2017–2019. The isolates were psychrophilic, oligotrophic, resistant to chloramphenicol,...
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ftmdpi:oai:mdpi.com:/2076-2607/10/4/704/ 2023-08-20T04:00:23+02:00 Characterisation of Waterborne Psychrophilic Massilia Isolates with Violacein Production and Description of Massilia antarctica sp. nov. Ivo Sedláček Pavla Holochová Hans-Jürgen Busse Vendula Koublová Stanislava Králová Pavel Švec Roman Sobotka Eva Staňková Jan Pilný Ondrej Šedo Jana Smolíková Karel Sedlář agris 2022-03-24 application/pdf https://doi.org/10.3390/microorganisms10040704 EN eng Multidisciplinary Digital Publishing Institute Environmental Microbiology https://dx.doi.org/10.3390/microorganisms10040704 https://creativecommons.org/licenses/by/4.0/ Microorganisms; Volume 10; Issue 4; Pages: 704 Massilia violacein psychrophilic description whole-genome sequencing Antarctica Text 2022 ftmdpi https://doi.org/10.3390/microorganisms10040704 2023-08-01T04:33:46Z A group of seven bacterial strains producing blue-purple pigmented colonies on R2A agar was isolated from freshwater samples collected in a deglaciated part of James Ross Island and Eagle Island, Antarctica, from 2017–2019. The isolates were psychrophilic, oligotrophic, resistant to chloramphenicol, and exhibited strong hydrolytic activities. To clarify the taxonomic position of these isolates, a polyphasic taxonomic approach was applied based on sequencing of the 16S rRNA, gyrB and lepA genes, whole-genome sequencing, rep-PCR, MALDI-TOF MS, chemotaxonomy analyses and biotyping. Phylogenetic analysis of the 16S rRNA gene sequences revealed that the entire group are representatives of the genus Massilia. The closest relatives of the reference strain P8398T were Massilia atriviolacea, Massilia violaceinigra, Massilia rubra, Massilia mucilaginosa, Massilia aquatica, Massilia frigida, Massilia glaciei and Massilia eurypsychrophila with a pairwise similarity of 98.6–100% in the 16S rRNA. The subsequent gyrB and lepA sequencing results showed the novelty of the analysed group, and the average nucleotide identity and digital DNA–DNA hybridisation values clearly proved that P8398T represents a distinct Massilia species. After all these results, we nominate a new species with the proposed name Massilia antarctica sp. nov. The type strain is P8398T (= CCM 8941T = LMG 32108T). Text Antarc* Antarctica Eagle Island James Ross Island Ross Island MDPI Open Access Publishing Ross Island Eagle Island ENVELOPE(-57.489,-57.489,-63.660,-63.660) Microorganisms 10 4 704 |
institution |
Open Polar |
collection |
MDPI Open Access Publishing |
op_collection_id |
ftmdpi |
language |
English |
topic |
Massilia violacein psychrophilic description whole-genome sequencing Antarctica |
spellingShingle |
Massilia violacein psychrophilic description whole-genome sequencing Antarctica Ivo Sedláček Pavla Holochová Hans-Jürgen Busse Vendula Koublová Stanislava Králová Pavel Švec Roman Sobotka Eva Staňková Jan Pilný Ondrej Šedo Jana Smolíková Karel Sedlář Characterisation of Waterborne Psychrophilic Massilia Isolates with Violacein Production and Description of Massilia antarctica sp. nov. |
topic_facet |
Massilia violacein psychrophilic description whole-genome sequencing Antarctica |
description |
A group of seven bacterial strains producing blue-purple pigmented colonies on R2A agar was isolated from freshwater samples collected in a deglaciated part of James Ross Island and Eagle Island, Antarctica, from 2017–2019. The isolates were psychrophilic, oligotrophic, resistant to chloramphenicol, and exhibited strong hydrolytic activities. To clarify the taxonomic position of these isolates, a polyphasic taxonomic approach was applied based on sequencing of the 16S rRNA, gyrB and lepA genes, whole-genome sequencing, rep-PCR, MALDI-TOF MS, chemotaxonomy analyses and biotyping. Phylogenetic analysis of the 16S rRNA gene sequences revealed that the entire group are representatives of the genus Massilia. The closest relatives of the reference strain P8398T were Massilia atriviolacea, Massilia violaceinigra, Massilia rubra, Massilia mucilaginosa, Massilia aquatica, Massilia frigida, Massilia glaciei and Massilia eurypsychrophila with a pairwise similarity of 98.6–100% in the 16S rRNA. The subsequent gyrB and lepA sequencing results showed the novelty of the analysed group, and the average nucleotide identity and digital DNA–DNA hybridisation values clearly proved that P8398T represents a distinct Massilia species. After all these results, we nominate a new species with the proposed name Massilia antarctica sp. nov. The type strain is P8398T (= CCM 8941T = LMG 32108T). |
format |
Text |
author |
Ivo Sedláček Pavla Holochová Hans-Jürgen Busse Vendula Koublová Stanislava Králová Pavel Švec Roman Sobotka Eva Staňková Jan Pilný Ondrej Šedo Jana Smolíková Karel Sedlář |
author_facet |
Ivo Sedláček Pavla Holochová Hans-Jürgen Busse Vendula Koublová Stanislava Králová Pavel Švec Roman Sobotka Eva Staňková Jan Pilný Ondrej Šedo Jana Smolíková Karel Sedlář |
author_sort |
Ivo Sedláček |
title |
Characterisation of Waterborne Psychrophilic Massilia Isolates with Violacein Production and Description of Massilia antarctica sp. nov. |
title_short |
Characterisation of Waterborne Psychrophilic Massilia Isolates with Violacein Production and Description of Massilia antarctica sp. nov. |
title_full |
Characterisation of Waterborne Psychrophilic Massilia Isolates with Violacein Production and Description of Massilia antarctica sp. nov. |
title_fullStr |
Characterisation of Waterborne Psychrophilic Massilia Isolates with Violacein Production and Description of Massilia antarctica sp. nov. |
title_full_unstemmed |
Characterisation of Waterborne Psychrophilic Massilia Isolates with Violacein Production and Description of Massilia antarctica sp. nov. |
title_sort |
characterisation of waterborne psychrophilic massilia isolates with violacein production and description of massilia antarctica sp. nov. |
publisher |
Multidisciplinary Digital Publishing Institute |
publishDate |
2022 |
url |
https://doi.org/10.3390/microorganisms10040704 |
op_coverage |
agris |
long_lat |
ENVELOPE(-57.489,-57.489,-63.660,-63.660) |
geographic |
Ross Island Eagle Island |
geographic_facet |
Ross Island Eagle Island |
genre |
Antarc* Antarctica Eagle Island James Ross Island Ross Island |
genre_facet |
Antarc* Antarctica Eagle Island James Ross Island Ross Island |
op_source |
Microorganisms; Volume 10; Issue 4; Pages: 704 |
op_relation |
Environmental Microbiology https://dx.doi.org/10.3390/microorganisms10040704 |
op_rights |
https://creativecommons.org/licenses/by/4.0/ |
op_doi |
https://doi.org/10.3390/microorganisms10040704 |
container_title |
Microorganisms |
container_volume |
10 |
container_issue |
4 |
container_start_page |
704 |
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1774717912868716544 |