Production of Mannosylerythritol Lipids Using Oils from Oleaginous Microalgae: Two Sequential Microorganism Culture Approach
Mannosylerythritol lipids (MELs) are biosurfactants with excellent biochemical properties and a wide range of potential applications. However, most of the studies focusing on MELs high titre production have been relying in the use of vegetable oils with impact on the sustainability and process econo...
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ftmdpi:oai:mdpi.com:/2076-2607/10/12/2390/ 2023-08-20T04:01:22+02:00 Production of Mannosylerythritol Lipids Using Oils from Oleaginous Microalgae: Two Sequential Microorganism Culture Approach Miguel Nascimento Tiago Coelho Alberto Reis Luísa Gouveia Nuno Faria Frederico Ferreira agris 2022-12-02 application/pdf https://doi.org/10.3390/microorganisms10122390 EN eng Multidisciplinary Digital Publishing Institute Microbial Biotechnology https://dx.doi.org/10.3390/microorganisms10122390 https://creativecommons.org/licenses/by/4.0/ Microorganisms; Volume 10; Issue 12; Pages: 2390 oleaginous microalgae bioprocess mannosylerythritol lipids (MELs) Moesziomyces spp Text 2022 ftmdpi https://doi.org/10.3390/microorganisms10122390 2023-08-01T07:37:09Z Mannosylerythritol lipids (MELs) are biosurfactants with excellent biochemical properties and a wide range of potential applications. However, most of the studies focusing on MELs high titre production have been relying in the use of vegetable oils with impact on the sustainability and process economy. Herein, we report for the first time MELs production using oils produced from microalgae. The bio-oil was extracted from Neochloris oleoabundans and evaluated for their use as sole carbon source or in a co-substrate strategy, using as an additional carbon source D-glucose, on Moesziomyces spp. cultures to support cell growth and induce the production of MELs. Both Moesziomyces antarcticus and M. aphidis were able to grow and produce MELs using algae-derived bio-oils as a carbon source. Using a medium containing as carbon sources 40 g/L of D-glucose and 20 g/L of bio-oils, Moesziomyces antarcticus and M. aphidis produced 12.47 ± 0.28 and 5.72 ± 2.32 g/L of MELs, respectively. Interestingly, there are no significant differences in productivity when using oils from microalgae or vegetable oils as carbon sources. The MELs productivities achieved were 1.78 ± 0.04 and 1.99 ± 0.12 g/L/day, respectively, for M. antarcticus fed with algae-derived or vegetable oils. These results open new perspectives for the production of MELs in systems combining different microorganisms. Text Antarc* antarcticus MDPI Open Access Publishing Microorganisms 10 12 2390 |
institution |
Open Polar |
collection |
MDPI Open Access Publishing |
op_collection_id |
ftmdpi |
language |
English |
topic |
oleaginous microalgae bioprocess mannosylerythritol lipids (MELs) Moesziomyces spp |
spellingShingle |
oleaginous microalgae bioprocess mannosylerythritol lipids (MELs) Moesziomyces spp Miguel Nascimento Tiago Coelho Alberto Reis Luísa Gouveia Nuno Faria Frederico Ferreira Production of Mannosylerythritol Lipids Using Oils from Oleaginous Microalgae: Two Sequential Microorganism Culture Approach |
topic_facet |
oleaginous microalgae bioprocess mannosylerythritol lipids (MELs) Moesziomyces spp |
description |
Mannosylerythritol lipids (MELs) are biosurfactants with excellent biochemical properties and a wide range of potential applications. However, most of the studies focusing on MELs high titre production have been relying in the use of vegetable oils with impact on the sustainability and process economy. Herein, we report for the first time MELs production using oils produced from microalgae. The bio-oil was extracted from Neochloris oleoabundans and evaluated for their use as sole carbon source or in a co-substrate strategy, using as an additional carbon source D-glucose, on Moesziomyces spp. cultures to support cell growth and induce the production of MELs. Both Moesziomyces antarcticus and M. aphidis were able to grow and produce MELs using algae-derived bio-oils as a carbon source. Using a medium containing as carbon sources 40 g/L of D-glucose and 20 g/L of bio-oils, Moesziomyces antarcticus and M. aphidis produced 12.47 ± 0.28 and 5.72 ± 2.32 g/L of MELs, respectively. Interestingly, there are no significant differences in productivity when using oils from microalgae or vegetable oils as carbon sources. The MELs productivities achieved were 1.78 ± 0.04 and 1.99 ± 0.12 g/L/day, respectively, for M. antarcticus fed with algae-derived or vegetable oils. These results open new perspectives for the production of MELs in systems combining different microorganisms. |
format |
Text |
author |
Miguel Nascimento Tiago Coelho Alberto Reis Luísa Gouveia Nuno Faria Frederico Ferreira |
author_facet |
Miguel Nascimento Tiago Coelho Alberto Reis Luísa Gouveia Nuno Faria Frederico Ferreira |
author_sort |
Miguel Nascimento |
title |
Production of Mannosylerythritol Lipids Using Oils from Oleaginous Microalgae: Two Sequential Microorganism Culture Approach |
title_short |
Production of Mannosylerythritol Lipids Using Oils from Oleaginous Microalgae: Two Sequential Microorganism Culture Approach |
title_full |
Production of Mannosylerythritol Lipids Using Oils from Oleaginous Microalgae: Two Sequential Microorganism Culture Approach |
title_fullStr |
Production of Mannosylerythritol Lipids Using Oils from Oleaginous Microalgae: Two Sequential Microorganism Culture Approach |
title_full_unstemmed |
Production of Mannosylerythritol Lipids Using Oils from Oleaginous Microalgae: Two Sequential Microorganism Culture Approach |
title_sort |
production of mannosylerythritol lipids using oils from oleaginous microalgae: two sequential microorganism culture approach |
publisher |
Multidisciplinary Digital Publishing Institute |
publishDate |
2022 |
url |
https://doi.org/10.3390/microorganisms10122390 |
op_coverage |
agris |
genre |
Antarc* antarcticus |
genre_facet |
Antarc* antarcticus |
op_source |
Microorganisms; Volume 10; Issue 12; Pages: 2390 |
op_relation |
Microbial Biotechnology https://dx.doi.org/10.3390/microorganisms10122390 |
op_rights |
https://creativecommons.org/licenses/by/4.0/ |
op_doi |
https://doi.org/10.3390/microorganisms10122390 |
container_title |
Microorganisms |
container_volume |
10 |
container_issue |
12 |
container_start_page |
2390 |
_version_ |
1774724660652408832 |