Study on the Effect of Relaying on Norovirus Reduction from Crassostrea gigas Oysters
Norovirus (NoV) is the most important cause of seafood-borne gastroenteritis worldwide, mainly associated with the consumption of raw oysters. NoV is often present in oysters that comply with existing control standards for shellfish. Therefore, the improvement of post-harvest treatments and practice...
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Online Access: | https://doi.org/10.3390/microorganisms10122389 |
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ftmdpi:oai:mdpi.com:/2076-2607/10/12/2389/ 2023-08-20T04:06:02+02:00 Study on the Effect of Relaying on Norovirus Reduction from Crassostrea gigas Oysters Roberta Battistini Chiara Masotti Cristiana Maurella Erica Costa Mino Orlandi Mirvana Feletti Carlo Ercolini Laura Serracca agris 2022-12-01 application/pdf https://doi.org/10.3390/microorganisms10122389 EN eng Multidisciplinary Digital Publishing Institute Food Microbiology https://dx.doi.org/10.3390/microorganisms10122389 https://creativecommons.org/licenses/by/4.0/ Microorganisms; Volume 10; Issue 12; Pages: 2389 Crassostrea gigas relay norovirus food safety real-time qPCR Text 2022 ftmdpi https://doi.org/10.3390/microorganisms10122389 2023-08-01T07:36:53Z Norovirus (NoV) is the most important cause of seafood-borne gastroenteritis worldwide, mainly associated with the consumption of raw oysters. NoV is often present in oysters that comply with existing control standards for shellfish. Therefore, the improvement of post-harvest treatments and practices can represent one of the main strategies to reduce the incidence of viral diseases related to shellfish. This study aimed to investigate long-term relays for the reduction of NoV levels in live oysters, during the high-risk cold months, by transferring the oysters from a more contaminated site to two sites with lower NoV levels. The efficacy of relaying was evaluated by analyzing oyster samples collected at days 0 (T0) and 30 (T30) for NoV levels using a real-time quantitative PCR (RT-qPCR). The NoV level at the relay sites was consistently lower than at the harvest site. The relay process for 30 days in seawater with a lower NoV level resulted in a decrease in the NoV load compared to day 0 with significant reductions depending on the site and genogroup of NoV considered. These results suggest that long-term relaying of oysters to reduce NoV levels is promising and could help growers to improve oyster safety; however, further investigations are needed. Text Crassostrea gigas MDPI Open Access Publishing Microorganisms 10 12 2389 |
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English |
topic |
Crassostrea gigas relay norovirus food safety real-time qPCR |
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Crassostrea gigas relay norovirus food safety real-time qPCR Roberta Battistini Chiara Masotti Cristiana Maurella Erica Costa Mino Orlandi Mirvana Feletti Carlo Ercolini Laura Serracca Study on the Effect of Relaying on Norovirus Reduction from Crassostrea gigas Oysters |
topic_facet |
Crassostrea gigas relay norovirus food safety real-time qPCR |
description |
Norovirus (NoV) is the most important cause of seafood-borne gastroenteritis worldwide, mainly associated with the consumption of raw oysters. NoV is often present in oysters that comply with existing control standards for shellfish. Therefore, the improvement of post-harvest treatments and practices can represent one of the main strategies to reduce the incidence of viral diseases related to shellfish. This study aimed to investigate long-term relays for the reduction of NoV levels in live oysters, during the high-risk cold months, by transferring the oysters from a more contaminated site to two sites with lower NoV levels. The efficacy of relaying was evaluated by analyzing oyster samples collected at days 0 (T0) and 30 (T30) for NoV levels using a real-time quantitative PCR (RT-qPCR). The NoV level at the relay sites was consistently lower than at the harvest site. The relay process for 30 days in seawater with a lower NoV level resulted in a decrease in the NoV load compared to day 0 with significant reductions depending on the site and genogroup of NoV considered. These results suggest that long-term relaying of oysters to reduce NoV levels is promising and could help growers to improve oyster safety; however, further investigations are needed. |
format |
Text |
author |
Roberta Battistini Chiara Masotti Cristiana Maurella Erica Costa Mino Orlandi Mirvana Feletti Carlo Ercolini Laura Serracca |
author_facet |
Roberta Battistini Chiara Masotti Cristiana Maurella Erica Costa Mino Orlandi Mirvana Feletti Carlo Ercolini Laura Serracca |
author_sort |
Roberta Battistini |
title |
Study on the Effect of Relaying on Norovirus Reduction from Crassostrea gigas Oysters |
title_short |
Study on the Effect of Relaying on Norovirus Reduction from Crassostrea gigas Oysters |
title_full |
Study on the Effect of Relaying on Norovirus Reduction from Crassostrea gigas Oysters |
title_fullStr |
Study on the Effect of Relaying on Norovirus Reduction from Crassostrea gigas Oysters |
title_full_unstemmed |
Study on the Effect of Relaying on Norovirus Reduction from Crassostrea gigas Oysters |
title_sort |
study on the effect of relaying on norovirus reduction from crassostrea gigas oysters |
publisher |
Multidisciplinary Digital Publishing Institute |
publishDate |
2022 |
url |
https://doi.org/10.3390/microorganisms10122389 |
op_coverage |
agris |
genre |
Crassostrea gigas |
genre_facet |
Crassostrea gigas |
op_source |
Microorganisms; Volume 10; Issue 12; Pages: 2389 |
op_relation |
Food Microbiology https://dx.doi.org/10.3390/microorganisms10122389 |
op_rights |
https://creativecommons.org/licenses/by/4.0/ |
op_doi |
https://doi.org/10.3390/microorganisms10122389 |
container_title |
Microorganisms |
container_volume |
10 |
container_issue |
12 |
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2389 |
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