The Viral Fraction Metatranscriptomes of Lake Baikal
This article characterises viral fraction metatranscriptomes (smaller than 0.2 µm) from the pelagic zone of oligotrophic Lake Baikal (Russia). The study revealed the dominance of transcripts of DNA viruses: bacteriophages and algal viruses. We identified transcripts similar to Pithovirus sibericum,...
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ftmdpi:oai:mdpi.com:/2076-2607/10/10/1937/ 2023-08-20T04:09:13+02:00 The Viral Fraction Metatranscriptomes of Lake Baikal Sergey Potapov Andrey Krasnopeev Irina Tikhonova Galina Podlesnaya Anna Gorshkova Olga Belykh agris 2022-09-29 application/pdf https://doi.org/10.3390/microorganisms10101937 EN eng Multidisciplinary Digital Publishing Institute Environmental Microbiology https://dx.doi.org/10.3390/microorganisms10101937 https://creativecommons.org/licenses/by/4.0/ Microorganisms; Volume 10; Issue 10; Pages: 1937 metatranscriptome viral fraction Lake Baikal RNA and DNA viruses Text 2022 ftmdpi https://doi.org/10.3390/microorganisms10101937 2023-08-01T06:41:42Z This article characterises viral fraction metatranscriptomes (smaller than 0.2 µm) from the pelagic zone of oligotrophic Lake Baikal (Russia). The study revealed the dominance of transcripts of DNA viruses: bacteriophages and algal viruses. We identified transcripts similar to Pithovirus sibericum, a nucleocytoplasmic large DNA virus (NCLDV) isolated from the permafrost region of Eastern Siberia. Among the families detected were RNA viruses assigned to Retroviridae, Metaviridae, Potyviridae, Astroviridae, and Closteroviridae. Using the PHROG, SEED subsystems databases, and the VOGDB, we indicated that the bulk of transcripts belong to the functional replication of viruses. In a comparative unweighted pair group method with arithmetic mean (UPGMA) analysis, the transcripts from Lake Baikal formed a separate cluster included in the clade with transcripts from other freshwater lakes, as well as marine and oceanic waters, while there was no separation based on the trophic state of the water bodies, the size of the plankton fraction, or salinity. Text permafrost Siberia MDPI Open Access Publishing Microorganisms 10 10 1937 |
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Open Polar |
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MDPI Open Access Publishing |
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ftmdpi |
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English |
topic |
metatranscriptome viral fraction Lake Baikal RNA and DNA viruses |
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metatranscriptome viral fraction Lake Baikal RNA and DNA viruses Sergey Potapov Andrey Krasnopeev Irina Tikhonova Galina Podlesnaya Anna Gorshkova Olga Belykh The Viral Fraction Metatranscriptomes of Lake Baikal |
topic_facet |
metatranscriptome viral fraction Lake Baikal RNA and DNA viruses |
description |
This article characterises viral fraction metatranscriptomes (smaller than 0.2 µm) from the pelagic zone of oligotrophic Lake Baikal (Russia). The study revealed the dominance of transcripts of DNA viruses: bacteriophages and algal viruses. We identified transcripts similar to Pithovirus sibericum, a nucleocytoplasmic large DNA virus (NCLDV) isolated from the permafrost region of Eastern Siberia. Among the families detected were RNA viruses assigned to Retroviridae, Metaviridae, Potyviridae, Astroviridae, and Closteroviridae. Using the PHROG, SEED subsystems databases, and the VOGDB, we indicated that the bulk of transcripts belong to the functional replication of viruses. In a comparative unweighted pair group method with arithmetic mean (UPGMA) analysis, the transcripts from Lake Baikal formed a separate cluster included in the clade with transcripts from other freshwater lakes, as well as marine and oceanic waters, while there was no separation based on the trophic state of the water bodies, the size of the plankton fraction, or salinity. |
format |
Text |
author |
Sergey Potapov Andrey Krasnopeev Irina Tikhonova Galina Podlesnaya Anna Gorshkova Olga Belykh |
author_facet |
Sergey Potapov Andrey Krasnopeev Irina Tikhonova Galina Podlesnaya Anna Gorshkova Olga Belykh |
author_sort |
Sergey Potapov |
title |
The Viral Fraction Metatranscriptomes of Lake Baikal |
title_short |
The Viral Fraction Metatranscriptomes of Lake Baikal |
title_full |
The Viral Fraction Metatranscriptomes of Lake Baikal |
title_fullStr |
The Viral Fraction Metatranscriptomes of Lake Baikal |
title_full_unstemmed |
The Viral Fraction Metatranscriptomes of Lake Baikal |
title_sort |
viral fraction metatranscriptomes of lake baikal |
publisher |
Multidisciplinary Digital Publishing Institute |
publishDate |
2022 |
url |
https://doi.org/10.3390/microorganisms10101937 |
op_coverage |
agris |
genre |
permafrost Siberia |
genre_facet |
permafrost Siberia |
op_source |
Microorganisms; Volume 10; Issue 10; Pages: 1937 |
op_relation |
Environmental Microbiology https://dx.doi.org/10.3390/microorganisms10101937 |
op_rights |
https://creativecommons.org/licenses/by/4.0/ |
op_doi |
https://doi.org/10.3390/microorganisms10101937 |
container_title |
Microorganisms |
container_volume |
10 |
container_issue |
10 |
container_start_page |
1937 |
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1774722026150297600 |