Salmonella and Antimicrobial Resistance in Wild Rodents—True or False Threat?
Transmission of pathogenic and resistant bacteria from wildlife to the bacterial gene pool in nature affects the ecosystem. Hence, we studied intestine content of five wild rodent species: the yellow-necked wood mouse (Apodemus flavicollis, n = 121), striped field mouse (Apodemus agrarius, n = 75),...
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ftmdpi:oai:mdpi.com:/2076-0817/9/9/771/ 2023-08-20T04:05:59+02:00 Salmonella and Antimicrobial Resistance in Wild Rodents—True or False Threat? Magdalena Skarżyńska Magdalena Zając Ewelina Kamińska Arkadiusz Bomba Jacek Żmudzki Artur Jabłoński Dariusz Wasyl agris 2020-09-21 application/pdf https://doi.org/10.3390/pathogens9090771 EN eng Multidisciplinary Digital Publishing Institute Animal Pathogens https://dx.doi.org/10.3390/pathogens9090771 https://creativecommons.org/licenses/by/4.0/ Pathogens; Volume 9; Issue 9; Pages: 771 rodents AMR Salmonella genotype/phenotype discrepancy Text 2020 ftmdpi https://doi.org/10.3390/pathogens9090771 2023-08-01T00:09:00Z Transmission of pathogenic and resistant bacteria from wildlife to the bacterial gene pool in nature affects the ecosystem. Hence, we studied intestine content of five wild rodent species: the yellow-necked wood mouse (Apodemus flavicollis, n = 121), striped field mouse (Apodemus agrarius, n = 75), common vole (Microtus arvalis, n = 37), bank vole (Myodes glareolus, n = 3), and house mouse (Mus musculus, n = 1) to assess their potential role as an antimicrobial resistance (AMR) and Salmonella vector. The methods adopted from official AMR monitoring of slaughtered animals were applied and supplemented with colistin resistance screening. Whole-genome sequencing of obtained bacteria elucidated their epidemiological relationships and zoonotic potential. The study revealed no indications of public health relevance of wild rodents from the sampled area in Salmonella spread and their limited role in AMR dissemination. Of 263 recovered E. coli, the vast majority was pan-susceptible, and as few as 5 E. coli showed any resistance. In four colistin-resistant strains neither the known mcr genes nor known mutations in pmr genes were found. One of these strains was tetracycline-resistant due to tet(B). High diversity of virulence factors (n = 43) noted in tested strains including ibeA, cdtB, air, eilA, astA, vat, pic reported in clinically relevant types of enteric E. coli indicate that rodents may be involved in the ecological cycle of these bacteria. Most of the strains represented unique sequence types and ST10805, ST10806, ST10810, ST10824 were revealed for the first time, showing genomic heterogeneity of the strains. The study broadened the knowledge on phylogenetic diversity and structure of the E. coli population in wild rodents. Text Common vole Microtus arvalis MDPI Open Access Publishing Pathogens 9 9 771 |
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MDPI Open Access Publishing |
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English |
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rodents AMR Salmonella genotype/phenotype discrepancy |
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rodents AMR Salmonella genotype/phenotype discrepancy Magdalena Skarżyńska Magdalena Zając Ewelina Kamińska Arkadiusz Bomba Jacek Żmudzki Artur Jabłoński Dariusz Wasyl Salmonella and Antimicrobial Resistance in Wild Rodents—True or False Threat? |
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rodents AMR Salmonella genotype/phenotype discrepancy |
description |
Transmission of pathogenic and resistant bacteria from wildlife to the bacterial gene pool in nature affects the ecosystem. Hence, we studied intestine content of five wild rodent species: the yellow-necked wood mouse (Apodemus flavicollis, n = 121), striped field mouse (Apodemus agrarius, n = 75), common vole (Microtus arvalis, n = 37), bank vole (Myodes glareolus, n = 3), and house mouse (Mus musculus, n = 1) to assess their potential role as an antimicrobial resistance (AMR) and Salmonella vector. The methods adopted from official AMR monitoring of slaughtered animals were applied and supplemented with colistin resistance screening. Whole-genome sequencing of obtained bacteria elucidated their epidemiological relationships and zoonotic potential. The study revealed no indications of public health relevance of wild rodents from the sampled area in Salmonella spread and their limited role in AMR dissemination. Of 263 recovered E. coli, the vast majority was pan-susceptible, and as few as 5 E. coli showed any resistance. In four colistin-resistant strains neither the known mcr genes nor known mutations in pmr genes were found. One of these strains was tetracycline-resistant due to tet(B). High diversity of virulence factors (n = 43) noted in tested strains including ibeA, cdtB, air, eilA, astA, vat, pic reported in clinically relevant types of enteric E. coli indicate that rodents may be involved in the ecological cycle of these bacteria. Most of the strains represented unique sequence types and ST10805, ST10806, ST10810, ST10824 were revealed for the first time, showing genomic heterogeneity of the strains. The study broadened the knowledge on phylogenetic diversity and structure of the E. coli population in wild rodents. |
format |
Text |
author |
Magdalena Skarżyńska Magdalena Zając Ewelina Kamińska Arkadiusz Bomba Jacek Żmudzki Artur Jabłoński Dariusz Wasyl |
author_facet |
Magdalena Skarżyńska Magdalena Zając Ewelina Kamińska Arkadiusz Bomba Jacek Żmudzki Artur Jabłoński Dariusz Wasyl |
author_sort |
Magdalena Skarżyńska |
title |
Salmonella and Antimicrobial Resistance in Wild Rodents—True or False Threat? |
title_short |
Salmonella and Antimicrobial Resistance in Wild Rodents—True or False Threat? |
title_full |
Salmonella and Antimicrobial Resistance in Wild Rodents—True or False Threat? |
title_fullStr |
Salmonella and Antimicrobial Resistance in Wild Rodents—True or False Threat? |
title_full_unstemmed |
Salmonella and Antimicrobial Resistance in Wild Rodents—True or False Threat? |
title_sort |
salmonella and antimicrobial resistance in wild rodents—true or false threat? |
publisher |
Multidisciplinary Digital Publishing Institute |
publishDate |
2020 |
url |
https://doi.org/10.3390/pathogens9090771 |
op_coverage |
agris |
genre |
Common vole Microtus arvalis |
genre_facet |
Common vole Microtus arvalis |
op_source |
Pathogens; Volume 9; Issue 9; Pages: 771 |
op_relation |
Animal Pathogens https://dx.doi.org/10.3390/pathogens9090771 |
op_rights |
https://creativecommons.org/licenses/by/4.0/ |
op_doi |
https://doi.org/10.3390/pathogens9090771 |
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Pathogens |
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9 |
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9 |
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771 |
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