Synthetic Peptides as a Promising Alternative to Control Viral Infections in Atlantic Salmon
Viral infections in salmonids represent an ongoing challenge for the aquaculture industry. Two RNA viruses, the infectious pancreatic necrosis virus (IPNV) and the infectious salmon anemia virus (ISAV), have become a latent risk without healing therapies available for either. In this context, antivi...
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ftmdpi:oai:mdpi.com:/2076-0817/9/8/600/ 2023-08-20T04:05:19+02:00 Synthetic Peptides as a Promising Alternative to Control Viral Infections in Atlantic Salmon Constanza Cárdenas Fanny Guzmán Marisela Carmona Cristian Muñoz Luis Nilo Alvaro Labra Sergio H. Marshall agris 2020-07-23 application/pdf https://doi.org/10.3390/pathogens9080600 EN eng Multidisciplinary Digital Publishing Institute Viral Pathogens https://dx.doi.org/10.3390/pathogens9080600 https://creativecommons.org/licenses/by/4.0/ Pathogens; Volume 9; Issue 8; Pages: 600 interfering peptides viral treatment RNA fish viruses Text 2020 ftmdpi https://doi.org/10.3390/pathogens9080600 2023-07-31T23:48:53Z Viral infections in salmonids represent an ongoing challenge for the aquaculture industry. Two RNA viruses, the infectious pancreatic necrosis virus (IPNV) and the infectious salmon anemia virus (ISAV), have become a latent risk without healing therapies available for either. In this context, antiviral peptides emerge as effective and relatively safe therapeutic molecules. Based on in silico analysis of VP2 protein from IPNV and the RNA-dependent RNA polymerase from ISAV, a set of peptides was designed and were chemically synthesized to block selected key events in their corresponding infectivity processes. The peptides were tested in fish cell lines in vitro, and four were selected for decreasing the viral load: peptide GIM182 for IPNV, and peptides GIM535, GIM538 and GIM539 for ISAV. In vivo tests with the IPNV GIM 182 peptide were carried out using Salmo salar fish, showing a significant decrease of viral load, and proving the safety of the peptide for fish. The results indicate that the use of peptides as antiviral agents in disease control might be a viable alternative to explore in aquaculture. Text Atlantic salmon Salmo salar MDPI Open Access Publishing Pathogens 9 8 600 |
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MDPI Open Access Publishing |
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ftmdpi |
language |
English |
topic |
interfering peptides viral treatment RNA fish viruses |
spellingShingle |
interfering peptides viral treatment RNA fish viruses Constanza Cárdenas Fanny Guzmán Marisela Carmona Cristian Muñoz Luis Nilo Alvaro Labra Sergio H. Marshall Synthetic Peptides as a Promising Alternative to Control Viral Infections in Atlantic Salmon |
topic_facet |
interfering peptides viral treatment RNA fish viruses |
description |
Viral infections in salmonids represent an ongoing challenge for the aquaculture industry. Two RNA viruses, the infectious pancreatic necrosis virus (IPNV) and the infectious salmon anemia virus (ISAV), have become a latent risk without healing therapies available for either. In this context, antiviral peptides emerge as effective and relatively safe therapeutic molecules. Based on in silico analysis of VP2 protein from IPNV and the RNA-dependent RNA polymerase from ISAV, a set of peptides was designed and were chemically synthesized to block selected key events in their corresponding infectivity processes. The peptides were tested in fish cell lines in vitro, and four were selected for decreasing the viral load: peptide GIM182 for IPNV, and peptides GIM535, GIM538 and GIM539 for ISAV. In vivo tests with the IPNV GIM 182 peptide were carried out using Salmo salar fish, showing a significant decrease of viral load, and proving the safety of the peptide for fish. The results indicate that the use of peptides as antiviral agents in disease control might be a viable alternative to explore in aquaculture. |
format |
Text |
author |
Constanza Cárdenas Fanny Guzmán Marisela Carmona Cristian Muñoz Luis Nilo Alvaro Labra Sergio H. Marshall |
author_facet |
Constanza Cárdenas Fanny Guzmán Marisela Carmona Cristian Muñoz Luis Nilo Alvaro Labra Sergio H. Marshall |
author_sort |
Constanza Cárdenas |
title |
Synthetic Peptides as a Promising Alternative to Control Viral Infections in Atlantic Salmon |
title_short |
Synthetic Peptides as a Promising Alternative to Control Viral Infections in Atlantic Salmon |
title_full |
Synthetic Peptides as a Promising Alternative to Control Viral Infections in Atlantic Salmon |
title_fullStr |
Synthetic Peptides as a Promising Alternative to Control Viral Infections in Atlantic Salmon |
title_full_unstemmed |
Synthetic Peptides as a Promising Alternative to Control Viral Infections in Atlantic Salmon |
title_sort |
synthetic peptides as a promising alternative to control viral infections in atlantic salmon |
publisher |
Multidisciplinary Digital Publishing Institute |
publishDate |
2020 |
url |
https://doi.org/10.3390/pathogens9080600 |
op_coverage |
agris |
genre |
Atlantic salmon Salmo salar |
genre_facet |
Atlantic salmon Salmo salar |
op_source |
Pathogens; Volume 9; Issue 8; Pages: 600 |
op_relation |
Viral Pathogens https://dx.doi.org/10.3390/pathogens9080600 |
op_rights |
https://creativecommons.org/licenses/by/4.0/ |
op_doi |
https://doi.org/10.3390/pathogens9080600 |
container_title |
Pathogens |
container_volume |
9 |
container_issue |
8 |
container_start_page |
600 |
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1774715821035094016 |