Spatial and Temporal Evolutionary Patterns in Puumala Orthohantavirus (PUUV) S Segment
The S segment of bank vole (Clethrionomys glareolus)-associated Puumala orthohantavirus (PUUV) contains two overlapping open reading frames coding for the nucleocapsid (N) and a non-structural (NSs) protein. To identify the influence of bank vole population dynamics on PUUV S segment sequence evolut...
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ftmdpi:oai:mdpi.com:/2076-0817/9/7/548/ 2023-08-20T04:07:59+02:00 Spatial and Temporal Evolutionary Patterns in Puumala Orthohantavirus (PUUV) S Segment Florian Binder René Ryll Stephan Drewes Sandra Jagdmann Daniela Reil Melanie Hiltbrunner Ulrike M. Rosenfeld Christian Imholt Jens Jacob Gerald Heckel Rainer G. Ulrich agris 2020-07-08 application/pdf https://doi.org/10.3390/pathogens9070548 EN eng Multidisciplinary Digital Publishing Institute https://dx.doi.org/10.3390/pathogens9070548 https://creativecommons.org/licenses/by/4.0/ Pathogens; Volume 9; Issue 7; Pages: 548 hantavirus bank vole evolution N protein NSs protein S segment Text 2020 ftmdpi https://doi.org/10.3390/pathogens9070548 2023-07-31T23:44:44Z The S segment of bank vole (Clethrionomys glareolus)-associated Puumala orthohantavirus (PUUV) contains two overlapping open reading frames coding for the nucleocapsid (N) and a non-structural (NSs) protein. To identify the influence of bank vole population dynamics on PUUV S segment sequence evolution and test for spillover infections in sympatric rodent species, during 2010–2014, 883 bank voles, 357 yellow-necked mice (Apodemus flavicollis), 62 wood mice (A. sylvaticus), 149 common voles (Microtus arvalis) and 8 field voles (M. agrestis) were collected in Baden-Wuerttemberg and North Rhine-Westphalia, Germany. In total, 27.9% and 22.3% of bank voles were positive for PUUV-reactive antibodies and PUUV-specific RNA, respectively. One of eight field voles was PUUV RNA-positive, indicating a spillover infection, but none of the other species showed evidence of PUUV infection. Phylogenetic and isolation-by-distance analyses demonstrated a spatial clustering of PUUV S segment sequences. In the hantavirus outbreak years 2010 and 2012, PUUV RNA prevalence was higher in our study regions compared to non-outbreak years 2011, 2013 and 2014. NSs amino acid and nucleotide sequence types showed temporal and/or local variation, whereas the N protein was highly conserved in the NSs overlapping region and, to a lower rate, in the N alone coding part. Text Microtus arvalis MDPI Open Access Publishing Pathogens 9 7 548 |
institution |
Open Polar |
collection |
MDPI Open Access Publishing |
op_collection_id |
ftmdpi |
language |
English |
topic |
hantavirus bank vole evolution N protein NSs protein S segment |
spellingShingle |
hantavirus bank vole evolution N protein NSs protein S segment Florian Binder René Ryll Stephan Drewes Sandra Jagdmann Daniela Reil Melanie Hiltbrunner Ulrike M. Rosenfeld Christian Imholt Jens Jacob Gerald Heckel Rainer G. Ulrich Spatial and Temporal Evolutionary Patterns in Puumala Orthohantavirus (PUUV) S Segment |
topic_facet |
hantavirus bank vole evolution N protein NSs protein S segment |
description |
The S segment of bank vole (Clethrionomys glareolus)-associated Puumala orthohantavirus (PUUV) contains two overlapping open reading frames coding for the nucleocapsid (N) and a non-structural (NSs) protein. To identify the influence of bank vole population dynamics on PUUV S segment sequence evolution and test for spillover infections in sympatric rodent species, during 2010–2014, 883 bank voles, 357 yellow-necked mice (Apodemus flavicollis), 62 wood mice (A. sylvaticus), 149 common voles (Microtus arvalis) and 8 field voles (M. agrestis) were collected in Baden-Wuerttemberg and North Rhine-Westphalia, Germany. In total, 27.9% and 22.3% of bank voles were positive for PUUV-reactive antibodies and PUUV-specific RNA, respectively. One of eight field voles was PUUV RNA-positive, indicating a spillover infection, but none of the other species showed evidence of PUUV infection. Phylogenetic and isolation-by-distance analyses demonstrated a spatial clustering of PUUV S segment sequences. In the hantavirus outbreak years 2010 and 2012, PUUV RNA prevalence was higher in our study regions compared to non-outbreak years 2011, 2013 and 2014. NSs amino acid and nucleotide sequence types showed temporal and/or local variation, whereas the N protein was highly conserved in the NSs overlapping region and, to a lower rate, in the N alone coding part. |
format |
Text |
author |
Florian Binder René Ryll Stephan Drewes Sandra Jagdmann Daniela Reil Melanie Hiltbrunner Ulrike M. Rosenfeld Christian Imholt Jens Jacob Gerald Heckel Rainer G. Ulrich |
author_facet |
Florian Binder René Ryll Stephan Drewes Sandra Jagdmann Daniela Reil Melanie Hiltbrunner Ulrike M. Rosenfeld Christian Imholt Jens Jacob Gerald Heckel Rainer G. Ulrich |
author_sort |
Florian Binder |
title |
Spatial and Temporal Evolutionary Patterns in Puumala Orthohantavirus (PUUV) S Segment |
title_short |
Spatial and Temporal Evolutionary Patterns in Puumala Orthohantavirus (PUUV) S Segment |
title_full |
Spatial and Temporal Evolutionary Patterns in Puumala Orthohantavirus (PUUV) S Segment |
title_fullStr |
Spatial and Temporal Evolutionary Patterns in Puumala Orthohantavirus (PUUV) S Segment |
title_full_unstemmed |
Spatial and Temporal Evolutionary Patterns in Puumala Orthohantavirus (PUUV) S Segment |
title_sort |
spatial and temporal evolutionary patterns in puumala orthohantavirus (puuv) s segment |
publisher |
Multidisciplinary Digital Publishing Institute |
publishDate |
2020 |
url |
https://doi.org/10.3390/pathogens9070548 |
op_coverage |
agris |
genre |
Microtus arvalis |
genre_facet |
Microtus arvalis |
op_source |
Pathogens; Volume 9; Issue 7; Pages: 548 |
op_relation |
https://dx.doi.org/10.3390/pathogens9070548 |
op_rights |
https://creativecommons.org/licenses/by/4.0/ |
op_doi |
https://doi.org/10.3390/pathogens9070548 |
container_title |
Pathogens |
container_volume |
9 |
container_issue |
7 |
container_start_page |
548 |
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