Screening of Fish Cell Lines for Piscine Orthoreovirus-1 (PRV-1) Amplification: Identification of the Non-Supportive PRV-1 Invitrome
Piscine reovirus (PRV) is the causative agent of heart and skeletal muscle inflammation (HSMI), which is detrimental to Atlantic Salmon (AS) aquaculture, but so far has not been cultivatable, which impedes studying the disease and developing a vaccine. Homogenates of head kidney and red blood cells...
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ftmdpi:oai:mdpi.com:/2076-0817/9/10/833/ 2023-08-20T04:05:18+02:00 Screening of Fish Cell Lines for Piscine Orthoreovirus-1 (PRV-1) Amplification: Identification of the Non-Supportive PRV-1 Invitrome Phuc H. Pham Ehab Misk Fotini Papazotos Ginny Jones Mark P. Polinski Elena Contador Spencer Russell Kyle A. Garver John S. Lumsden Niels C. Bols agris 2020-10-12 application/pdf https://doi.org/10.3390/pathogens9100833 EN eng Multidisciplinary Digital Publishing Institute https://dx.doi.org/10.3390/pathogens9100833 https://creativecommons.org/licenses/by/4.0/ Pathogens; Volume 9; Issue 10; Pages: 833 piscine reovirus PRV-1 fish cell lines non-supportive heart and skeletal muscle inflammation HSMI invitrome RNA virus Atlantic salmon Text 2020 ftmdpi https://doi.org/10.3390/pathogens9100833 2023-08-01T00:15:21Z Piscine reovirus (PRV) is the causative agent of heart and skeletal muscle inflammation (HSMI), which is detrimental to Atlantic Salmon (AS) aquaculture, but so far has not been cultivatable, which impedes studying the disease and developing a vaccine. Homogenates of head kidney and red blood cells (RBC) from AS in which PRV-1 had been detected were applied to fish cell lines. The cell lines were from embryos, and from brain, blood, fin, gill, gonads, gut, heart, kidney, liver, skin, and spleen, and had the shapes of endothelial, epithelial, fibroblast, and macrophage cells. Most cell lines were derived from the Neopterygii subclass of fish, but one was from subclass Chondrostei. Cultures were examined by phase contrast microscopy for appearance, and by quantitative polymerase chain reaction (qPCR) for PRV-1 RNA amplification and for the capacity to transfer any changes to new cultures. No changes in appearance and Ct values were observed consistently or transferable to new cultures. Therefore, 31 cell lines examined were unable to support PRV-1 amplification and are described as belonging to the non-supportive PRV-1 invitrome. However, these investigations and cell lines can contribute to understanding PRV-1 cellular and host tropism, and the interactions between virus-infected and bystander cells. Text Atlantic salmon MDPI Open Access Publishing Pathogens 9 10 833 |
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MDPI Open Access Publishing |
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ftmdpi |
language |
English |
topic |
piscine reovirus PRV-1 fish cell lines non-supportive heart and skeletal muscle inflammation HSMI invitrome RNA virus Atlantic salmon |
spellingShingle |
piscine reovirus PRV-1 fish cell lines non-supportive heart and skeletal muscle inflammation HSMI invitrome RNA virus Atlantic salmon Phuc H. Pham Ehab Misk Fotini Papazotos Ginny Jones Mark P. Polinski Elena Contador Spencer Russell Kyle A. Garver John S. Lumsden Niels C. Bols Screening of Fish Cell Lines for Piscine Orthoreovirus-1 (PRV-1) Amplification: Identification of the Non-Supportive PRV-1 Invitrome |
topic_facet |
piscine reovirus PRV-1 fish cell lines non-supportive heart and skeletal muscle inflammation HSMI invitrome RNA virus Atlantic salmon |
description |
Piscine reovirus (PRV) is the causative agent of heart and skeletal muscle inflammation (HSMI), which is detrimental to Atlantic Salmon (AS) aquaculture, but so far has not been cultivatable, which impedes studying the disease and developing a vaccine. Homogenates of head kidney and red blood cells (RBC) from AS in which PRV-1 had been detected were applied to fish cell lines. The cell lines were from embryos, and from brain, blood, fin, gill, gonads, gut, heart, kidney, liver, skin, and spleen, and had the shapes of endothelial, epithelial, fibroblast, and macrophage cells. Most cell lines were derived from the Neopterygii subclass of fish, but one was from subclass Chondrostei. Cultures were examined by phase contrast microscopy for appearance, and by quantitative polymerase chain reaction (qPCR) for PRV-1 RNA amplification and for the capacity to transfer any changes to new cultures. No changes in appearance and Ct values were observed consistently or transferable to new cultures. Therefore, 31 cell lines examined were unable to support PRV-1 amplification and are described as belonging to the non-supportive PRV-1 invitrome. However, these investigations and cell lines can contribute to understanding PRV-1 cellular and host tropism, and the interactions between virus-infected and bystander cells. |
format |
Text |
author |
Phuc H. Pham Ehab Misk Fotini Papazotos Ginny Jones Mark P. Polinski Elena Contador Spencer Russell Kyle A. Garver John S. Lumsden Niels C. Bols |
author_facet |
Phuc H. Pham Ehab Misk Fotini Papazotos Ginny Jones Mark P. Polinski Elena Contador Spencer Russell Kyle A. Garver John S. Lumsden Niels C. Bols |
author_sort |
Phuc H. Pham |
title |
Screening of Fish Cell Lines for Piscine Orthoreovirus-1 (PRV-1) Amplification: Identification of the Non-Supportive PRV-1 Invitrome |
title_short |
Screening of Fish Cell Lines for Piscine Orthoreovirus-1 (PRV-1) Amplification: Identification of the Non-Supportive PRV-1 Invitrome |
title_full |
Screening of Fish Cell Lines for Piscine Orthoreovirus-1 (PRV-1) Amplification: Identification of the Non-Supportive PRV-1 Invitrome |
title_fullStr |
Screening of Fish Cell Lines for Piscine Orthoreovirus-1 (PRV-1) Amplification: Identification of the Non-Supportive PRV-1 Invitrome |
title_full_unstemmed |
Screening of Fish Cell Lines for Piscine Orthoreovirus-1 (PRV-1) Amplification: Identification of the Non-Supportive PRV-1 Invitrome |
title_sort |
screening of fish cell lines for piscine orthoreovirus-1 (prv-1) amplification: identification of the non-supportive prv-1 invitrome |
publisher |
Multidisciplinary Digital Publishing Institute |
publishDate |
2020 |
url |
https://doi.org/10.3390/pathogens9100833 |
op_coverage |
agris |
genre |
Atlantic salmon |
genre_facet |
Atlantic salmon |
op_source |
Pathogens; Volume 9; Issue 10; Pages: 833 |
op_relation |
https://dx.doi.org/10.3390/pathogens9100833 |
op_rights |
https://creativecommons.org/licenses/by/4.0/ |
op_doi |
https://doi.org/10.3390/pathogens9100833 |
container_title |
Pathogens |
container_volume |
9 |
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10 |
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833 |
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1774715792447766528 |