Transcriptome Response of Atlantic Salmon (Salmo salar) to a New Piscine Orthomyxovirus
Pilchard orthomyxovirus (POMV) is an emerging pathogen of concern to the salmon industry in Australia. To explore the molecular events that underpin POMV infection, we challenged Atlantic salmon (Salmo salar) post-smolts in seawater via cohabitation. Tissue samples of the head kidney and liver were...
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ftmdpi:oai:mdpi.com:/2076-0817/9/10/807/ 2023-08-20T04:05:16+02:00 Transcriptome Response of Atlantic Salmon (Salmo salar) to a New Piscine Orthomyxovirus Francisca Samsing Pamela Alexandre Megan Rigby Richard S. Taylor Roger Chong James W. Wynne agris 2020-09-30 application/pdf https://doi.org/10.3390/pathogens9100807 EN eng Multidisciplinary Digital Publishing Institute Immunological Responses and Immune Defense Mechanisms https://dx.doi.org/10.3390/pathogens9100807 https://creativecommons.org/licenses/by/4.0/ Pathogens; Volume 9; Issue 10; Pages: 807 host–pathogen interactions interferon T cell-mediated immunity Pilchard orthomyxovirus (POMV) Text 2020 ftmdpi https://doi.org/10.3390/pathogens9100807 2023-08-01T00:12:21Z Pilchard orthomyxovirus (POMV) is an emerging pathogen of concern to the salmon industry in Australia. To explore the molecular events that underpin POMV infection, we challenged Atlantic salmon (Salmo salar) post-smolts in seawater via cohabitation. Tissue samples of the head kidney and liver were collected from moribund and surviving individuals and analyzed using transcriptome sequencing. Viral loads were higher in the head kidney compared to the liver, yet the liver presented more upregulated genes. Fish infected with POMV showed a strong innate immune response that included the upregulation of pathogen recognition receptors such as RIG-I and Toll-like receptors as well as the induction of interferon-stimulated genes (MX, ISG15). Moribund fish also presented a dramatic induction of pro-inflammatory cytokines, contributing to severe tissue damage and morbidity. An induction of major histocompatibility complex (MHC) class I genes (B2M) and markers of T cell-mediated immunity (CD8-alpha, CD8-beta, Perforin-1, Granzyme-A) was observed in both moribund fish and survivors. In addition, differential connectivity analysis showed that three key regulators (RELA/p65, PRDM1, and HLF) related to cell-mediated immunity had significant differences in connectivity in “clinically healthy” versus “clinically affected” or moribund fish. Collectively, our results show that T cell-mediated immunity plays a central role in the response of Atlantic salmon to the infection with POMV. Text Atlantic salmon Salmo salar MDPI Open Access Publishing Pathogens 9 10 807 |
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MDPI Open Access Publishing |
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ftmdpi |
language |
English |
topic |
host–pathogen interactions interferon T cell-mediated immunity Pilchard orthomyxovirus (POMV) |
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host–pathogen interactions interferon T cell-mediated immunity Pilchard orthomyxovirus (POMV) Francisca Samsing Pamela Alexandre Megan Rigby Richard S. Taylor Roger Chong James W. Wynne Transcriptome Response of Atlantic Salmon (Salmo salar) to a New Piscine Orthomyxovirus |
topic_facet |
host–pathogen interactions interferon T cell-mediated immunity Pilchard orthomyxovirus (POMV) |
description |
Pilchard orthomyxovirus (POMV) is an emerging pathogen of concern to the salmon industry in Australia. To explore the molecular events that underpin POMV infection, we challenged Atlantic salmon (Salmo salar) post-smolts in seawater via cohabitation. Tissue samples of the head kidney and liver were collected from moribund and surviving individuals and analyzed using transcriptome sequencing. Viral loads were higher in the head kidney compared to the liver, yet the liver presented more upregulated genes. Fish infected with POMV showed a strong innate immune response that included the upregulation of pathogen recognition receptors such as RIG-I and Toll-like receptors as well as the induction of interferon-stimulated genes (MX, ISG15). Moribund fish also presented a dramatic induction of pro-inflammatory cytokines, contributing to severe tissue damage and morbidity. An induction of major histocompatibility complex (MHC) class I genes (B2M) and markers of T cell-mediated immunity (CD8-alpha, CD8-beta, Perforin-1, Granzyme-A) was observed in both moribund fish and survivors. In addition, differential connectivity analysis showed that three key regulators (RELA/p65, PRDM1, and HLF) related to cell-mediated immunity had significant differences in connectivity in “clinically healthy” versus “clinically affected” or moribund fish. Collectively, our results show that T cell-mediated immunity plays a central role in the response of Atlantic salmon to the infection with POMV. |
format |
Text |
author |
Francisca Samsing Pamela Alexandre Megan Rigby Richard S. Taylor Roger Chong James W. Wynne |
author_facet |
Francisca Samsing Pamela Alexandre Megan Rigby Richard S. Taylor Roger Chong James W. Wynne |
author_sort |
Francisca Samsing |
title |
Transcriptome Response of Atlantic Salmon (Salmo salar) to a New Piscine Orthomyxovirus |
title_short |
Transcriptome Response of Atlantic Salmon (Salmo salar) to a New Piscine Orthomyxovirus |
title_full |
Transcriptome Response of Atlantic Salmon (Salmo salar) to a New Piscine Orthomyxovirus |
title_fullStr |
Transcriptome Response of Atlantic Salmon (Salmo salar) to a New Piscine Orthomyxovirus |
title_full_unstemmed |
Transcriptome Response of Atlantic Salmon (Salmo salar) to a New Piscine Orthomyxovirus |
title_sort |
transcriptome response of atlantic salmon (salmo salar) to a new piscine orthomyxovirus |
publisher |
Multidisciplinary Digital Publishing Institute |
publishDate |
2020 |
url |
https://doi.org/10.3390/pathogens9100807 |
op_coverage |
agris |
genre |
Atlantic salmon Salmo salar |
genre_facet |
Atlantic salmon Salmo salar |
op_source |
Pathogens; Volume 9; Issue 10; Pages: 807 |
op_relation |
Immunological Responses and Immune Defense Mechanisms https://dx.doi.org/10.3390/pathogens9100807 |
op_rights |
https://creativecommons.org/licenses/by/4.0/ |
op_doi |
https://doi.org/10.3390/pathogens9100807 |
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Pathogens |
container_volume |
9 |
container_issue |
10 |
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807 |
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