Multilocus Sequence Typing (MLST) and Random Polymorphic DNA (RAPD) Comparisons of Geographic Isolates of Neoparamoeba perurans, the Causative Agent of Amoebic Gill Disease
Neoparamoba perurans, is the aetiological agent of amoebic gill disease (AGD), a disease that affects farmed Atlantic salmon worldwide. Multilocus sequence typing (MLST) and Random Amplified Polymorphic DNA (RAPD) are PCR-based typing methods that allow for the highly reproducible genetic analysis o...
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ftmdpi:oai:mdpi.com:/2076-0817/8/4/244/ 2023-08-20T04:05:20+02:00 Multilocus Sequence Typing (MLST) and Random Polymorphic DNA (RAPD) Comparisons of Geographic Isolates of Neoparamoeba perurans, the Causative Agent of Amoebic Gill Disease Jessica C. Johnson-Mackinnon Philip B. B. Crosbie Egil Karlsbakk Mar Marcos-Lopez Richard Paley Barbara F. Nowak Andrew R. Bridle agris 2019-11-19 application/pdf https://doi.org/10.3390/pathogens8040244 EN eng Multidisciplinary Digital Publishing Institute Emerging Pathogens https://dx.doi.org/10.3390/pathogens8040244 https://creativecommons.org/licenses/by/4.0/ Pathogens; Volume 8; Issue 4; Pages: 244 Paramoeba perurans Amoebic Gill Disease epidemiology pathogen genetics Text 2019 ftmdpi https://doi.org/10.3390/pathogens8040244 2023-07-31T22:48:33Z Neoparamoba perurans, is the aetiological agent of amoebic gill disease (AGD), a disease that affects farmed Atlantic salmon worldwide. Multilocus sequence typing (MLST) and Random Amplified Polymorphic DNA (RAPD) are PCR-based typing methods that allow for the highly reproducible genetic analysis of population structure within microbial species. To the best of our knowledge, this study represents the first use of these typing methods applied to N. perurans with the objective of distinguishing geographical isolates. These analyses were applied to a total of 16 isolates from Australia, Canada, Ireland, Scotland, Norway, and the USA. All the samples from Australia came from farm sites on the island state of Tasmania. Genetic polymorphism among isolates was more evident from the RAPD analysis compared to the MLST that used conserved housekeeping genes. Both techniques consistently identified that isolates of N. perurans from Tasmania, Australia were more similar to each other than to the isolates from other countries. While genetic differences were identified between geographical isolates, a BURST analysis provided no evidence of a founder genotype. This suggests that emerging outbreaks of AGD are not due to rapid translocation of this important salmonid pathogen from the same area. Text Atlantic salmon MDPI Open Access Publishing Canada Norway Pathogens 8 4 244 |
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Open Polar |
collection |
MDPI Open Access Publishing |
op_collection_id |
ftmdpi |
language |
English |
topic |
Paramoeba perurans Amoebic Gill Disease epidemiology pathogen genetics |
spellingShingle |
Paramoeba perurans Amoebic Gill Disease epidemiology pathogen genetics Jessica C. Johnson-Mackinnon Philip B. B. Crosbie Egil Karlsbakk Mar Marcos-Lopez Richard Paley Barbara F. Nowak Andrew R. Bridle Multilocus Sequence Typing (MLST) and Random Polymorphic DNA (RAPD) Comparisons of Geographic Isolates of Neoparamoeba perurans, the Causative Agent of Amoebic Gill Disease |
topic_facet |
Paramoeba perurans Amoebic Gill Disease epidemiology pathogen genetics |
description |
Neoparamoba perurans, is the aetiological agent of amoebic gill disease (AGD), a disease that affects farmed Atlantic salmon worldwide. Multilocus sequence typing (MLST) and Random Amplified Polymorphic DNA (RAPD) are PCR-based typing methods that allow for the highly reproducible genetic analysis of population structure within microbial species. To the best of our knowledge, this study represents the first use of these typing methods applied to N. perurans with the objective of distinguishing geographical isolates. These analyses were applied to a total of 16 isolates from Australia, Canada, Ireland, Scotland, Norway, and the USA. All the samples from Australia came from farm sites on the island state of Tasmania. Genetic polymorphism among isolates was more evident from the RAPD analysis compared to the MLST that used conserved housekeeping genes. Both techniques consistently identified that isolates of N. perurans from Tasmania, Australia were more similar to each other than to the isolates from other countries. While genetic differences were identified between geographical isolates, a BURST analysis provided no evidence of a founder genotype. This suggests that emerging outbreaks of AGD are not due to rapid translocation of this important salmonid pathogen from the same area. |
format |
Text |
author |
Jessica C. Johnson-Mackinnon Philip B. B. Crosbie Egil Karlsbakk Mar Marcos-Lopez Richard Paley Barbara F. Nowak Andrew R. Bridle |
author_facet |
Jessica C. Johnson-Mackinnon Philip B. B. Crosbie Egil Karlsbakk Mar Marcos-Lopez Richard Paley Barbara F. Nowak Andrew R. Bridle |
author_sort |
Jessica C. Johnson-Mackinnon |
title |
Multilocus Sequence Typing (MLST) and Random Polymorphic DNA (RAPD) Comparisons of Geographic Isolates of Neoparamoeba perurans, the Causative Agent of Amoebic Gill Disease |
title_short |
Multilocus Sequence Typing (MLST) and Random Polymorphic DNA (RAPD) Comparisons of Geographic Isolates of Neoparamoeba perurans, the Causative Agent of Amoebic Gill Disease |
title_full |
Multilocus Sequence Typing (MLST) and Random Polymorphic DNA (RAPD) Comparisons of Geographic Isolates of Neoparamoeba perurans, the Causative Agent of Amoebic Gill Disease |
title_fullStr |
Multilocus Sequence Typing (MLST) and Random Polymorphic DNA (RAPD) Comparisons of Geographic Isolates of Neoparamoeba perurans, the Causative Agent of Amoebic Gill Disease |
title_full_unstemmed |
Multilocus Sequence Typing (MLST) and Random Polymorphic DNA (RAPD) Comparisons of Geographic Isolates of Neoparamoeba perurans, the Causative Agent of Amoebic Gill Disease |
title_sort |
multilocus sequence typing (mlst) and random polymorphic dna (rapd) comparisons of geographic isolates of neoparamoeba perurans, the causative agent of amoebic gill disease |
publisher |
Multidisciplinary Digital Publishing Institute |
publishDate |
2019 |
url |
https://doi.org/10.3390/pathogens8040244 |
op_coverage |
agris |
geographic |
Canada Norway |
geographic_facet |
Canada Norway |
genre |
Atlantic salmon |
genre_facet |
Atlantic salmon |
op_source |
Pathogens; Volume 8; Issue 4; Pages: 244 |
op_relation |
Emerging Pathogens https://dx.doi.org/10.3390/pathogens8040244 |
op_rights |
https://creativecommons.org/licenses/by/4.0/ |
op_doi |
https://doi.org/10.3390/pathogens8040244 |
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Pathogens |
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8 |
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4 |
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244 |
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