Detection and Quantification of Nocardia crassostreae, an Emerging Pathogen, in Mytilus galloprovincialis in the Mediterranean Sea Using Droplet Digital PCR
Nocardia crassostreae is a novel pathogen responsible for infections in oysters (Crassostrea gigas) and mussels (Mytilus galloprovincialis). N. crassostreae is also responsible for nocardiosis both in immunocompetent and immunocompromised patients. We investigated N. crassostreae DNA in mussels grow...
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ftmdpi:oai:mdpi.com:/2076-0817/12/8/994/ 2023-08-20T04:06:04+02:00 Detection and Quantification of Nocardia crassostreae, an Emerging Pathogen, in Mytilus galloprovincialis in the Mediterranean Sea Using Droplet Digital PCR Anna Cutarelli Francesca Carella Francesca De Falco Bianca Cuccaro Fabio Di Nocera Donatella Nava Gionata De Vico Sante Roperto agris 2023-07-28 application/pdf https://doi.org/10.3390/pathogens12080994 EN eng Multidisciplinary Digital Publishing Institute Emerging Pathogens https://dx.doi.org/10.3390/pathogens12080994 https://creativecommons.org/licenses/by/4.0/ Pathogens; Volume 12; Issue 8; Pages: 994 Nocardia crassostreae mussel Mytilus galloprovincialis Mediterranean Sea droplet digital PCR (ddPCR) real-time qPCR Text 2023 ftmdpi https://doi.org/10.3390/pathogens12080994 2023-08-01T11:04:02Z Nocardia crassostreae is a novel pathogen responsible for infections in oysters (Crassostrea gigas) and mussels (Mytilus galloprovincialis). N. crassostreae is also responsible for nocardiosis both in immunocompetent and immunocompromised patients. We investigated N. crassostreae DNA in mussels grown in marine sites of the Mediterranean Sea in the Campania Region. We examined 185 mussel pooled samples by droplet digital PCR (ddPCR) and real-time quantitative PCR (qPCR), each pool composed of 10 mussels and 149 individual mussels. ddPCR detected N. crassostreae DNA in 48 mussel pooled samples and in 23 individual mussel samples. qPCR detected N. crassostreae DNA in six pooled samples and six individual mussel samples. The two molecular assays for the detection of N. crassostreae DNA showed significant differences both in the pooled and in individual samples. Our study demonstrated that ddPCR outperformed real-time qPCR for N. crassostreae DNA detection, thus confirming that ddPCR technology can identify the pathogens in many infectious diseases with high sensitivity and specificity. Furthermore, in individual mussels showing histological lesions due to N. crassostreae, the lowest copy number/microliter detected by ddPCR of this pathogen was 0.3, which suggests that this dose could be enough to cause infections of N. crassostreae in mussels. Text Crassostrea gigas MDPI Open Access Publishing Pathogens 12 8 994 |
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English |
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Nocardia crassostreae mussel Mytilus galloprovincialis Mediterranean Sea droplet digital PCR (ddPCR) real-time qPCR |
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Nocardia crassostreae mussel Mytilus galloprovincialis Mediterranean Sea droplet digital PCR (ddPCR) real-time qPCR Anna Cutarelli Francesca Carella Francesca De Falco Bianca Cuccaro Fabio Di Nocera Donatella Nava Gionata De Vico Sante Roperto Detection and Quantification of Nocardia crassostreae, an Emerging Pathogen, in Mytilus galloprovincialis in the Mediterranean Sea Using Droplet Digital PCR |
topic_facet |
Nocardia crassostreae mussel Mytilus galloprovincialis Mediterranean Sea droplet digital PCR (ddPCR) real-time qPCR |
description |
Nocardia crassostreae is a novel pathogen responsible for infections in oysters (Crassostrea gigas) and mussels (Mytilus galloprovincialis). N. crassostreae is also responsible for nocardiosis both in immunocompetent and immunocompromised patients. We investigated N. crassostreae DNA in mussels grown in marine sites of the Mediterranean Sea in the Campania Region. We examined 185 mussel pooled samples by droplet digital PCR (ddPCR) and real-time quantitative PCR (qPCR), each pool composed of 10 mussels and 149 individual mussels. ddPCR detected N. crassostreae DNA in 48 mussel pooled samples and in 23 individual mussel samples. qPCR detected N. crassostreae DNA in six pooled samples and six individual mussel samples. The two molecular assays for the detection of N. crassostreae DNA showed significant differences both in the pooled and in individual samples. Our study demonstrated that ddPCR outperformed real-time qPCR for N. crassostreae DNA detection, thus confirming that ddPCR technology can identify the pathogens in many infectious diseases with high sensitivity and specificity. Furthermore, in individual mussels showing histological lesions due to N. crassostreae, the lowest copy number/microliter detected by ddPCR of this pathogen was 0.3, which suggests that this dose could be enough to cause infections of N. crassostreae in mussels. |
format |
Text |
author |
Anna Cutarelli Francesca Carella Francesca De Falco Bianca Cuccaro Fabio Di Nocera Donatella Nava Gionata De Vico Sante Roperto |
author_facet |
Anna Cutarelli Francesca Carella Francesca De Falco Bianca Cuccaro Fabio Di Nocera Donatella Nava Gionata De Vico Sante Roperto |
author_sort |
Anna Cutarelli |
title |
Detection and Quantification of Nocardia crassostreae, an Emerging Pathogen, in Mytilus galloprovincialis in the Mediterranean Sea Using Droplet Digital PCR |
title_short |
Detection and Quantification of Nocardia crassostreae, an Emerging Pathogen, in Mytilus galloprovincialis in the Mediterranean Sea Using Droplet Digital PCR |
title_full |
Detection and Quantification of Nocardia crassostreae, an Emerging Pathogen, in Mytilus galloprovincialis in the Mediterranean Sea Using Droplet Digital PCR |
title_fullStr |
Detection and Quantification of Nocardia crassostreae, an Emerging Pathogen, in Mytilus galloprovincialis in the Mediterranean Sea Using Droplet Digital PCR |
title_full_unstemmed |
Detection and Quantification of Nocardia crassostreae, an Emerging Pathogen, in Mytilus galloprovincialis in the Mediterranean Sea Using Droplet Digital PCR |
title_sort |
detection and quantification of nocardia crassostreae, an emerging pathogen, in mytilus galloprovincialis in the mediterranean sea using droplet digital pcr |
publisher |
Multidisciplinary Digital Publishing Institute |
publishDate |
2023 |
url |
https://doi.org/10.3390/pathogens12080994 |
op_coverage |
agris |
genre |
Crassostrea gigas |
genre_facet |
Crassostrea gigas |
op_source |
Pathogens; Volume 12; Issue 8; Pages: 994 |
op_relation |
Emerging Pathogens https://dx.doi.org/10.3390/pathogens12080994 |
op_rights |
https://creativecommons.org/licenses/by/4.0/ |
op_doi |
https://doi.org/10.3390/pathogens12080994 |
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Pathogens |
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994 |
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