Molecular Confirmation of Accipiter Birds of Prey as Definitive Hosts of Numerous Sarcocystis Species, including Sarcocystis sp., Closely Related to Pathogenic S. calchasi
The present study aimed to test intestinal scrapings of the Northern Goshawk (Accipiter gentilis) and the Eurasian Sparrowhawk (Accipiter nisus) from Lithuania for S. calchasi and other Sarcocystis species characterised by bird–bird life cycles. The protozoan parasite Sarcocystis calchasi can cause...
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2023
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ftmdpi:oai:mdpi.com:/2076-0817/12/6/752/ 2023-08-20T03:59:02+02:00 Molecular Confirmation of Accipiter Birds of Prey as Definitive Hosts of Numerous Sarcocystis Species, including Sarcocystis sp., Closely Related to Pathogenic S. calchasi Tautvilė Šukytė Dalius Butkauskas Evelina Juozaitytė-Ngugu Saulius Švažas Petras Prakas agris 2023-05-23 application/pdf https://doi.org/10.3390/pathogens12060752 EN eng Multidisciplinary Digital Publishing Institute Parasitic Pathogens https://dx.doi.org/10.3390/pathogens12060752 https://creativecommons.org/licenses/by/4.0/ Pathogens; Volume 12; Issue 6; Pages: 752 Sarcocystis Sarcocystis calchasi Accipiter hawks DNA analysis life cycle predator–prey ITS1 phylogeny Text 2023 ftmdpi https://doi.org/10.3390/pathogens12060752 2023-08-01T10:11:28Z The present study aimed to test intestinal scrapings of the Northern Goshawk (Accipiter gentilis) and the Eurasian Sparrowhawk (Accipiter nisus) from Lithuania for S. calchasi and other Sarcocystis species characterised by bird–bird life cycles. The protozoan parasite Sarcocystis calchasi can cause respiratory and neurological diseases in a variety of birds; however, the distribution of this parasite is not well-examined. Sarcocystis species were identified with nested PCR and sequencing of the partial ITS1 region. Sporocysts and/or sporulated oocysts of Sarcocystis spp. were observed in 16 (100%) Northern Goshawks and 9 (56.3%) Eurasian Sparrowhawks. Four species, S. columbae, S. halieti, S. turdusi, and S. wobeseri, were confirmed in the Eurasian Sparrowhawk. Apart from the latter four species, S. calchasi, S. cornixi, S. kutkienae, and S. lari were established in the Northern Goshawk. A higher prevalence of Sarcocystis spp. and species richness in Northern Goshawks is associated with the differences in the diet of two examined Accipiter species. This study is the first report of S. calchasi in Lithuania. Furthermore, the genetically distinct species Sarcocystis spp. 23LTAcc, which is most closely related to S. calchasi, was found in three Northern Goshawks. Text Accipiter gentilis Northern Goshawk MDPI Open Access Publishing Pathogens 12 6 752 |
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Open Polar |
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MDPI Open Access Publishing |
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ftmdpi |
language |
English |
topic |
Sarcocystis Sarcocystis calchasi Accipiter hawks DNA analysis life cycle predator–prey ITS1 phylogeny |
spellingShingle |
Sarcocystis Sarcocystis calchasi Accipiter hawks DNA analysis life cycle predator–prey ITS1 phylogeny Tautvilė Šukytė Dalius Butkauskas Evelina Juozaitytė-Ngugu Saulius Švažas Petras Prakas Molecular Confirmation of Accipiter Birds of Prey as Definitive Hosts of Numerous Sarcocystis Species, including Sarcocystis sp., Closely Related to Pathogenic S. calchasi |
topic_facet |
Sarcocystis Sarcocystis calchasi Accipiter hawks DNA analysis life cycle predator–prey ITS1 phylogeny |
description |
The present study aimed to test intestinal scrapings of the Northern Goshawk (Accipiter gentilis) and the Eurasian Sparrowhawk (Accipiter nisus) from Lithuania for S. calchasi and other Sarcocystis species characterised by bird–bird life cycles. The protozoan parasite Sarcocystis calchasi can cause respiratory and neurological diseases in a variety of birds; however, the distribution of this parasite is not well-examined. Sarcocystis species were identified with nested PCR and sequencing of the partial ITS1 region. Sporocysts and/or sporulated oocysts of Sarcocystis spp. were observed in 16 (100%) Northern Goshawks and 9 (56.3%) Eurasian Sparrowhawks. Four species, S. columbae, S. halieti, S. turdusi, and S. wobeseri, were confirmed in the Eurasian Sparrowhawk. Apart from the latter four species, S. calchasi, S. cornixi, S. kutkienae, and S. lari were established in the Northern Goshawk. A higher prevalence of Sarcocystis spp. and species richness in Northern Goshawks is associated with the differences in the diet of two examined Accipiter species. This study is the first report of S. calchasi in Lithuania. Furthermore, the genetically distinct species Sarcocystis spp. 23LTAcc, which is most closely related to S. calchasi, was found in three Northern Goshawks. |
format |
Text |
author |
Tautvilė Šukytė Dalius Butkauskas Evelina Juozaitytė-Ngugu Saulius Švažas Petras Prakas |
author_facet |
Tautvilė Šukytė Dalius Butkauskas Evelina Juozaitytė-Ngugu Saulius Švažas Petras Prakas |
author_sort |
Tautvilė Šukytė |
title |
Molecular Confirmation of Accipiter Birds of Prey as Definitive Hosts of Numerous Sarcocystis Species, including Sarcocystis sp., Closely Related to Pathogenic S. calchasi |
title_short |
Molecular Confirmation of Accipiter Birds of Prey as Definitive Hosts of Numerous Sarcocystis Species, including Sarcocystis sp., Closely Related to Pathogenic S. calchasi |
title_full |
Molecular Confirmation of Accipiter Birds of Prey as Definitive Hosts of Numerous Sarcocystis Species, including Sarcocystis sp., Closely Related to Pathogenic S. calchasi |
title_fullStr |
Molecular Confirmation of Accipiter Birds of Prey as Definitive Hosts of Numerous Sarcocystis Species, including Sarcocystis sp., Closely Related to Pathogenic S. calchasi |
title_full_unstemmed |
Molecular Confirmation of Accipiter Birds of Prey as Definitive Hosts of Numerous Sarcocystis Species, including Sarcocystis sp., Closely Related to Pathogenic S. calchasi |
title_sort |
molecular confirmation of accipiter birds of prey as definitive hosts of numerous sarcocystis species, including sarcocystis sp., closely related to pathogenic s. calchasi |
publisher |
Multidisciplinary Digital Publishing Institute |
publishDate |
2023 |
url |
https://doi.org/10.3390/pathogens12060752 |
op_coverage |
agris |
genre |
Accipiter gentilis Northern Goshawk |
genre_facet |
Accipiter gentilis Northern Goshawk |
op_source |
Pathogens; Volume 12; Issue 6; Pages: 752 |
op_relation |
Parasitic Pathogens https://dx.doi.org/10.3390/pathogens12060752 |
op_rights |
https://creativecommons.org/licenses/by/4.0/ |
op_doi |
https://doi.org/10.3390/pathogens12060752 |
container_title |
Pathogens |
container_volume |
12 |
container_issue |
6 |
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752 |
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1774723968455933952 |