Molecular Detection and Phylogenetic Analysis of Riemerella anatipestifer in Poultry and Wild Geese in Poland
Riemerella anatipestifer (RA) is one of the most relevant bacterial pathogens of commercial waterfowl from clinical and economic points of view. Our study aimed to evaluate the prevalence of RA infection in different types of commercial poultry in Poland and verify the potential role of wild geese a...
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ftmdpi:oai:mdpi.com:/2076-0817/12/2/256/ 2023-08-20T04:10:06+02:00 Molecular Detection and Phylogenetic Analysis of Riemerella anatipestifer in Poultry and Wild Geese in Poland Anna Sawicka-Durkalec Grzegorz Tomczyk Iryna Gerilovych Olimpia Kursa agris 2023-02-05 application/pdf https://doi.org/10.3390/pathogens12020256 EN eng Multidisciplinary Digital Publishing Institute Emerging Pathogens https://dx.doi.org/10.3390/pathogens12020256 https://creativecommons.org/licenses/by/4.0/ Pathogens; Volume 12; Issue 2; Pages: 256 Riemerella anatipestifer wild geese poultry phylogenetic analysis Text 2023 ftmdpi https://doi.org/10.3390/pathogens12020256 2023-08-01T08:39:10Z Riemerella anatipestifer (RA) is one of the most relevant bacterial pathogens of commercial waterfowl from clinical and economic points of view. Our study aimed to evaluate the prevalence of RA infection in different types of commercial poultry in Poland and verify the potential role of wild geese as vectors of this pathogen. We tested a total of 126 poultry flocks, including geese (N = 20), ducks (N = 42), turkeys (N = 64) and 19 wild geese, including greater white-fronted geese (N = 9), greylag geese (N = 5) and Taiga bean geese (N = 5). Tracheal swabs were examined for RA using a PCR targeting a conserved region of the 16S rRNA gene. Selected PCR products were sequenced to perform the phylogenetic analysis. Among the commercial poultry, the highest RA prevalence was found in flocks of ducks (35.7%) and geese (30.0%), whereas the lowest one was found in turkeys (3.2%). Most tested wild geese (94.7%) were RA positive. The phylogenetic analysis showed relatively low genetic diversity of the sequences analyzed, which gathered in two clusters of the phylogenetic tree, and the minimum nucleotide identity was 98.6%. Our results would support the contention that RA isolates from commercial poultry circulate in wild bird populations but are not transmitted back to poultry. Text taiga MDPI Open Access Publishing Pathogens 12 2 256 |
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Riemerella anatipestifer wild geese poultry phylogenetic analysis |
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Riemerella anatipestifer wild geese poultry phylogenetic analysis Anna Sawicka-Durkalec Grzegorz Tomczyk Iryna Gerilovych Olimpia Kursa Molecular Detection and Phylogenetic Analysis of Riemerella anatipestifer in Poultry and Wild Geese in Poland |
topic_facet |
Riemerella anatipestifer wild geese poultry phylogenetic analysis |
description |
Riemerella anatipestifer (RA) is one of the most relevant bacterial pathogens of commercial waterfowl from clinical and economic points of view. Our study aimed to evaluate the prevalence of RA infection in different types of commercial poultry in Poland and verify the potential role of wild geese as vectors of this pathogen. We tested a total of 126 poultry flocks, including geese (N = 20), ducks (N = 42), turkeys (N = 64) and 19 wild geese, including greater white-fronted geese (N = 9), greylag geese (N = 5) and Taiga bean geese (N = 5). Tracheal swabs were examined for RA using a PCR targeting a conserved region of the 16S rRNA gene. Selected PCR products were sequenced to perform the phylogenetic analysis. Among the commercial poultry, the highest RA prevalence was found in flocks of ducks (35.7%) and geese (30.0%), whereas the lowest one was found in turkeys (3.2%). Most tested wild geese (94.7%) were RA positive. The phylogenetic analysis showed relatively low genetic diversity of the sequences analyzed, which gathered in two clusters of the phylogenetic tree, and the minimum nucleotide identity was 98.6%. Our results would support the contention that RA isolates from commercial poultry circulate in wild bird populations but are not transmitted back to poultry. |
format |
Text |
author |
Anna Sawicka-Durkalec Grzegorz Tomczyk Iryna Gerilovych Olimpia Kursa |
author_facet |
Anna Sawicka-Durkalec Grzegorz Tomczyk Iryna Gerilovych Olimpia Kursa |
author_sort |
Anna Sawicka-Durkalec |
title |
Molecular Detection and Phylogenetic Analysis of Riemerella anatipestifer in Poultry and Wild Geese in Poland |
title_short |
Molecular Detection and Phylogenetic Analysis of Riemerella anatipestifer in Poultry and Wild Geese in Poland |
title_full |
Molecular Detection and Phylogenetic Analysis of Riemerella anatipestifer in Poultry and Wild Geese in Poland |
title_fullStr |
Molecular Detection and Phylogenetic Analysis of Riemerella anatipestifer in Poultry and Wild Geese in Poland |
title_full_unstemmed |
Molecular Detection and Phylogenetic Analysis of Riemerella anatipestifer in Poultry and Wild Geese in Poland |
title_sort |
molecular detection and phylogenetic analysis of riemerella anatipestifer in poultry and wild geese in poland |
publisher |
Multidisciplinary Digital Publishing Institute |
publishDate |
2023 |
url |
https://doi.org/10.3390/pathogens12020256 |
op_coverage |
agris |
genre |
taiga |
genre_facet |
taiga |
op_source |
Pathogens; Volume 12; Issue 2; Pages: 256 |
op_relation |
Emerging Pathogens https://dx.doi.org/10.3390/pathogens12020256 |
op_rights |
https://creativecommons.org/licenses/by/4.0/ |
op_doi |
https://doi.org/10.3390/pathogens12020256 |
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Pathogens |
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12 |
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2 |
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256 |
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1774724047162048512 |