The First Record of Echinococcus ortleppi (G5) Tapeworms in Grey Wolf (Canis lupus)
The aim of this study is to confirm the presence and molecular identification of Echinococcus tapeworms in wolves from south-eastern Poland. An investigation was carried out on the intestines of 13 wolves from south-eastern Poland. The small intestines were divided into three equal segments. Each se...
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ftmdpi:oai:mdpi.com:/2076-0817/10/7/853/ 2023-08-20T04:05:46+02:00 The First Record of Echinococcus ortleppi (G5) Tapeworms in Grey Wolf (Canis lupus) Jacek Karamon Małgorzata Samorek-Pieróg Jacek Sroka Ewa Bilska-Zając Joanna Dąbrowska Maciej Kochanowski Mirosław Różycki Jolanta Zdybel Tomasz Cencek agris 2021-07-06 application/pdf https://doi.org/10.3390/pathogens10070853 EN eng Multidisciplinary Digital Publishing Institute Parasitic Pathogens https://dx.doi.org/10.3390/pathogens10070853 https://creativecommons.org/licenses/by/4.0/ Pathogens; Volume 10; Issue 7; Pages: 853 wolf Canis lupus Echinococcus granulosus Echinococcus ortleppi Poland Text 2021 ftmdpi https://doi.org/10.3390/pathogens10070853 2023-08-01T02:07:50Z The aim of this study is to confirm the presence and molecular identification of Echinococcus tapeworms in wolves from south-eastern Poland. An investigation was carried out on the intestines of 13 wolves from south-eastern Poland. The small intestines were divided into three equal segments. Each segment was separately examined using the sedimentation and counting technique (SCT). The detected Echinococcus tapeworms were isolated and identified by PCRs and sequencing (nad1 and cox1 genes). Additionally, DNA isolated from the feces of wolves positive for Echinococcus tapeworms was examined with two diagnostic PCRs. The intestines of one wolf were positive for E. granulosus s.l. when assessed by SCT; the intestine was from a six-year-old male wolf killed in a communication accident. We detected 61 adult tapeworms: 42 in the anterior, 14 in the middle, and 5 in the posterior parts of the small intestine. The PCRs conducted for cox1 and nad1 produced specific products. A sequence comparison with the GenBank database showed similarity to the deposited E. ortleppi (G5) sequences. An analysis of the available phylogenetic sequences showed very little variation within the species of E. ortleppi (G5), and identity ranged from 99.10% to 100.00% in the case of cox1 and from 99.04% to 100.00% in the case of nad1. One of the two diagnostic PCRs used and performed on the feces of Echinococcus-positive animals showed product specific for E. granulosus. This study showed the presence of adult E. ortleppi tapeworms in wolves for the first time. Text Canis lupus MDPI Open Access Publishing Pathogens 10 7 853 |
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Open Polar |
collection |
MDPI Open Access Publishing |
op_collection_id |
ftmdpi |
language |
English |
topic |
wolf Canis lupus Echinococcus granulosus Echinococcus ortleppi Poland |
spellingShingle |
wolf Canis lupus Echinococcus granulosus Echinococcus ortleppi Poland Jacek Karamon Małgorzata Samorek-Pieróg Jacek Sroka Ewa Bilska-Zając Joanna Dąbrowska Maciej Kochanowski Mirosław Różycki Jolanta Zdybel Tomasz Cencek The First Record of Echinococcus ortleppi (G5) Tapeworms in Grey Wolf (Canis lupus) |
topic_facet |
wolf Canis lupus Echinococcus granulosus Echinococcus ortleppi Poland |
description |
The aim of this study is to confirm the presence and molecular identification of Echinococcus tapeworms in wolves from south-eastern Poland. An investigation was carried out on the intestines of 13 wolves from south-eastern Poland. The small intestines were divided into three equal segments. Each segment was separately examined using the sedimentation and counting technique (SCT). The detected Echinococcus tapeworms were isolated and identified by PCRs and sequencing (nad1 and cox1 genes). Additionally, DNA isolated from the feces of wolves positive for Echinococcus tapeworms was examined with two diagnostic PCRs. The intestines of one wolf were positive for E. granulosus s.l. when assessed by SCT; the intestine was from a six-year-old male wolf killed in a communication accident. We detected 61 adult tapeworms: 42 in the anterior, 14 in the middle, and 5 in the posterior parts of the small intestine. The PCRs conducted for cox1 and nad1 produced specific products. A sequence comparison with the GenBank database showed similarity to the deposited E. ortleppi (G5) sequences. An analysis of the available phylogenetic sequences showed very little variation within the species of E. ortleppi (G5), and identity ranged from 99.10% to 100.00% in the case of cox1 and from 99.04% to 100.00% in the case of nad1. One of the two diagnostic PCRs used and performed on the feces of Echinococcus-positive animals showed product specific for E. granulosus. This study showed the presence of adult E. ortleppi tapeworms in wolves for the first time. |
format |
Text |
author |
Jacek Karamon Małgorzata Samorek-Pieróg Jacek Sroka Ewa Bilska-Zając Joanna Dąbrowska Maciej Kochanowski Mirosław Różycki Jolanta Zdybel Tomasz Cencek |
author_facet |
Jacek Karamon Małgorzata Samorek-Pieróg Jacek Sroka Ewa Bilska-Zając Joanna Dąbrowska Maciej Kochanowski Mirosław Różycki Jolanta Zdybel Tomasz Cencek |
author_sort |
Jacek Karamon |
title |
The First Record of Echinococcus ortleppi (G5) Tapeworms in Grey Wolf (Canis lupus) |
title_short |
The First Record of Echinococcus ortleppi (G5) Tapeworms in Grey Wolf (Canis lupus) |
title_full |
The First Record of Echinococcus ortleppi (G5) Tapeworms in Grey Wolf (Canis lupus) |
title_fullStr |
The First Record of Echinococcus ortleppi (G5) Tapeworms in Grey Wolf (Canis lupus) |
title_full_unstemmed |
The First Record of Echinococcus ortleppi (G5) Tapeworms in Grey Wolf (Canis lupus) |
title_sort |
first record of echinococcus ortleppi (g5) tapeworms in grey wolf (canis lupus) |
publisher |
Multidisciplinary Digital Publishing Institute |
publishDate |
2021 |
url |
https://doi.org/10.3390/pathogens10070853 |
op_coverage |
agris |
genre |
Canis lupus |
genre_facet |
Canis lupus |
op_source |
Pathogens; Volume 10; Issue 7; Pages: 853 |
op_relation |
Parasitic Pathogens https://dx.doi.org/10.3390/pathogens10070853 |
op_rights |
https://creativecommons.org/licenses/by/4.0/ |
op_doi |
https://doi.org/10.3390/pathogens10070853 |
container_title |
Pathogens |
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10 |
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7 |
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853 |
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