Karyotypes and COI Gene Sequences of Chironomus sp. Le1 (Kiknadze and Salova, 1996), Ch. laetus (Belyanina and Filinkova, 1996) and Their Hybrid from the Yamal Peninsula, Arctic Zone of Russia
The study of the biological diversity of the Arctic zone yields intriguing results. Initial research on the lakes of the Yamal Peninsula resulted in the identification of Chironomus laetus and the hybrid Ch. laetus × Ch. sp. Le1. To avoid misidentification, we used morphological, cytogenetic, and mo...
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ftmdpi:oai:mdpi.com:/2075-4450/13/12/1112/ 2023-08-20T04:04:18+02:00 Karyotypes and COI Gene Sequences of Chironomus sp. Le1 (Kiknadze and Salova, 1996), Ch. laetus (Belyanina and Filinkova, 1996) and Their Hybrid from the Yamal Peninsula, Arctic Zone of Russia Viktor Bolshakov Alexander Prokin Dmitry Pavlov Azamat Akkizov Ekaterina Movergoz agris 2022-11-30 application/pdf https://doi.org/10.3390/insects13121112 EN eng Multidisciplinary Digital Publishing Institute Insect Molecular Biology and Genomics https://dx.doi.org/10.3390/insects13121112 https://creativecommons.org/licenses/by/4.0/ Insects; Volume 13; Issue 12; Pages: 1112 karyotype hybrid Chironomus Chironomus laetus Ch. sp. Le1 COI DNA-barcoding comparative cytogenetics Text 2022 ftmdpi https://doi.org/10.3390/insects13121112 2023-08-01T07:35:45Z The study of the biological diversity of the Arctic zone yields intriguing results. Initial research on the lakes of the Yamal Peninsula resulted in the identification of Chironomus laetus and the hybrid Ch. laetus × Ch. sp. Le1. To avoid misidentification, we used morphological, cytogenetic, and molecular genetic approaches. By cytogenetics, in Ch. sp. Le1, seven banding sequences were found: Le1A1, Le1B1, Le1C1, Le1D1, Le1E1, Le1F1, and Le1G1. The karyotype of Ch. laetus was mapped for the first time; it is the first species with the arm combinations AE BC DF G. We propose the name of a new cytocomplex—“laetus”. DNA-barcoding of the COI gene was carried out for Ch. laetus and Ch. laetus × Ch. sp. Le1 for the first time. The estimated genetic distance between the sequences of Ch. laetus and Ch. riihimakiensis is 2.3–2.5%. The high similarity in morphology, banding sequences, and the possibility of hybridization indicate a close relationship between Ch. laetus and Ch. sp. Le1, which is assumed to be the northern variant of Ch. riihimakiensis. Molecular genetic data suggests the presence of a subgroup with Ch. laetus. Text Arctic Yamal Peninsula MDPI Open Access Publishing Arctic Yamal Peninsula ENVELOPE(69.873,69.873,70.816,70.816) Insects 13 12 1112 |
institution |
Open Polar |
collection |
MDPI Open Access Publishing |
op_collection_id |
ftmdpi |
language |
English |
topic |
karyotype hybrid Chironomus Chironomus laetus Ch. sp. Le1 COI DNA-barcoding comparative cytogenetics |
spellingShingle |
karyotype hybrid Chironomus Chironomus laetus Ch. sp. Le1 COI DNA-barcoding comparative cytogenetics Viktor Bolshakov Alexander Prokin Dmitry Pavlov Azamat Akkizov Ekaterina Movergoz Karyotypes and COI Gene Sequences of Chironomus sp. Le1 (Kiknadze and Salova, 1996), Ch. laetus (Belyanina and Filinkova, 1996) and Their Hybrid from the Yamal Peninsula, Arctic Zone of Russia |
topic_facet |
karyotype hybrid Chironomus Chironomus laetus Ch. sp. Le1 COI DNA-barcoding comparative cytogenetics |
description |
The study of the biological diversity of the Arctic zone yields intriguing results. Initial research on the lakes of the Yamal Peninsula resulted in the identification of Chironomus laetus and the hybrid Ch. laetus × Ch. sp. Le1. To avoid misidentification, we used morphological, cytogenetic, and molecular genetic approaches. By cytogenetics, in Ch. sp. Le1, seven banding sequences were found: Le1A1, Le1B1, Le1C1, Le1D1, Le1E1, Le1F1, and Le1G1. The karyotype of Ch. laetus was mapped for the first time; it is the first species with the arm combinations AE BC DF G. We propose the name of a new cytocomplex—“laetus”. DNA-barcoding of the COI gene was carried out for Ch. laetus and Ch. laetus × Ch. sp. Le1 for the first time. The estimated genetic distance between the sequences of Ch. laetus and Ch. riihimakiensis is 2.3–2.5%. The high similarity in morphology, banding sequences, and the possibility of hybridization indicate a close relationship between Ch. laetus and Ch. sp. Le1, which is assumed to be the northern variant of Ch. riihimakiensis. Molecular genetic data suggests the presence of a subgroup with Ch. laetus. |
format |
Text |
author |
Viktor Bolshakov Alexander Prokin Dmitry Pavlov Azamat Akkizov Ekaterina Movergoz |
author_facet |
Viktor Bolshakov Alexander Prokin Dmitry Pavlov Azamat Akkizov Ekaterina Movergoz |
author_sort |
Viktor Bolshakov |
title |
Karyotypes and COI Gene Sequences of Chironomus sp. Le1 (Kiknadze and Salova, 1996), Ch. laetus (Belyanina and Filinkova, 1996) and Their Hybrid from the Yamal Peninsula, Arctic Zone of Russia |
title_short |
Karyotypes and COI Gene Sequences of Chironomus sp. Le1 (Kiknadze and Salova, 1996), Ch. laetus (Belyanina and Filinkova, 1996) and Their Hybrid from the Yamal Peninsula, Arctic Zone of Russia |
title_full |
Karyotypes and COI Gene Sequences of Chironomus sp. Le1 (Kiknadze and Salova, 1996), Ch. laetus (Belyanina and Filinkova, 1996) and Their Hybrid from the Yamal Peninsula, Arctic Zone of Russia |
title_fullStr |
Karyotypes and COI Gene Sequences of Chironomus sp. Le1 (Kiknadze and Salova, 1996), Ch. laetus (Belyanina and Filinkova, 1996) and Their Hybrid from the Yamal Peninsula, Arctic Zone of Russia |
title_full_unstemmed |
Karyotypes and COI Gene Sequences of Chironomus sp. Le1 (Kiknadze and Salova, 1996), Ch. laetus (Belyanina and Filinkova, 1996) and Their Hybrid from the Yamal Peninsula, Arctic Zone of Russia |
title_sort |
karyotypes and coi gene sequences of chironomus sp. le1 (kiknadze and salova, 1996), ch. laetus (belyanina and filinkova, 1996) and their hybrid from the yamal peninsula, arctic zone of russia |
publisher |
Multidisciplinary Digital Publishing Institute |
publishDate |
2022 |
url |
https://doi.org/10.3390/insects13121112 |
op_coverage |
agris |
long_lat |
ENVELOPE(69.873,69.873,70.816,70.816) |
geographic |
Arctic Yamal Peninsula |
geographic_facet |
Arctic Yamal Peninsula |
genre |
Arctic Yamal Peninsula |
genre_facet |
Arctic Yamal Peninsula |
op_source |
Insects; Volume 13; Issue 12; Pages: 1112 |
op_relation |
Insect Molecular Biology and Genomics https://dx.doi.org/10.3390/insects13121112 |
op_rights |
https://creativecommons.org/licenses/by/4.0/ |
op_doi |
https://doi.org/10.3390/insects13121112 |
container_title |
Insects |
container_volume |
13 |
container_issue |
12 |
container_start_page |
1112 |
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1774714688261586944 |