Metagenomic Analysis of Microbial Community Compositions and Cold-Responsive Stress Genes in Selected Antarctic Lacustrine and Soil Ecosystems
This study describes microbial community compositions, and various cold-responsive stress genes, encompassing cold-induced proteins (CIPs) and cold-associated general stress-responsive proteins (CASPs) in selected Antarctic lake water, sediment, and soil metagenomes. Overall, Proteobacteria and Bact...
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ftmdpi:oai:mdpi.com:/2075-1729/8/3/29/ 2023-08-20T04:02:00+02:00 Metagenomic Analysis of Microbial Community Compositions and Cold-Responsive Stress Genes in Selected Antarctic Lacustrine and Soil Ecosystems Hyunmin Koo Joseph Hakim Casey Morrow Michael Crowley Dale Andersen Asim Bej agris 2018-07-11 application/pdf https://doi.org/10.3390/life8030029 EN eng Multidisciplinary Digital Publishing Institute Microbiology https://dx.doi.org/10.3390/life8030029 https://creativecommons.org/licenses/by/4.0/ Life; Volume 8; Issue 3; Pages: 29 MG-RAST cold shock Lake Untersee Ace Lake Newcomb Bay Lake Mount Seuss McMurdo Dry Valleys Text 2018 ftmdpi https://doi.org/10.3390/life8030029 2023-07-31T21:37:17Z This study describes microbial community compositions, and various cold-responsive stress genes, encompassing cold-induced proteins (CIPs) and cold-associated general stress-responsive proteins (CASPs) in selected Antarctic lake water, sediment, and soil metagenomes. Overall, Proteobacteria and Bacteroidetes were the major taxa in all metagenomes. Prochlorococcus and Thiomicrospira were highly abundant in waters, while Myxococcus, Anaeromyxobacter, Haliangium, and Gloeobacter were dominant in the soil and lake sediment metagenomes. Among CIPs, genes necessary for DNA replication, translation initiation, and transcription termination were highly abundant in all metagenomes. However, genes for fatty acid desaturase (FAD) and trehalose synthase (TS) were common in the soil and lake sediment metagenomes. Interestingly, the Lake Untersee water and sediment metagenome samples contained histone-like nucleoid structuring protein (H-NS) and all genes for CIPs. As for the CASPs, high abundances of a wide range of genes for cryo- and osmo-protectants (glutamate, glycine, choline, and betaine) were identified in all metagenomes. However, genes for exopolysaccharide biosynthesis were dominant in Lake Untersee water, sediment, and other soil metagenomes. The results from this study indicate that although diverse microbial communities are present in various metagenomes, they share common cold-responsive stress genes necessary for their survival and sustenance in the extreme Antarctic conditions. Text Antarc* Antarctic McMurdo Dry Valleys MDPI Open Access Publishing Ace Lake ENVELOPE(78.188,78.188,-68.472,-68.472) Antarctic Bay Lake ENVELOPE(-100.964,-100.964,56.759,56.759) McMurdo Dry Valleys Newcomb Bay ENVELOPE(110.533,110.533,-66.267,-66.267) Untersee ENVELOPE(13.467,13.467,-71.350,-71.350) Life 8 3 29 |
institution |
Open Polar |
collection |
MDPI Open Access Publishing |
op_collection_id |
ftmdpi |
language |
English |
topic |
MG-RAST cold shock Lake Untersee Ace Lake Newcomb Bay Lake Mount Seuss McMurdo Dry Valleys |
spellingShingle |
MG-RAST cold shock Lake Untersee Ace Lake Newcomb Bay Lake Mount Seuss McMurdo Dry Valleys Hyunmin Koo Joseph Hakim Casey Morrow Michael Crowley Dale Andersen Asim Bej Metagenomic Analysis of Microbial Community Compositions and Cold-Responsive Stress Genes in Selected Antarctic Lacustrine and Soil Ecosystems |
topic_facet |
MG-RAST cold shock Lake Untersee Ace Lake Newcomb Bay Lake Mount Seuss McMurdo Dry Valleys |
description |
This study describes microbial community compositions, and various cold-responsive stress genes, encompassing cold-induced proteins (CIPs) and cold-associated general stress-responsive proteins (CASPs) in selected Antarctic lake water, sediment, and soil metagenomes. Overall, Proteobacteria and Bacteroidetes were the major taxa in all metagenomes. Prochlorococcus and Thiomicrospira were highly abundant in waters, while Myxococcus, Anaeromyxobacter, Haliangium, and Gloeobacter were dominant in the soil and lake sediment metagenomes. Among CIPs, genes necessary for DNA replication, translation initiation, and transcription termination were highly abundant in all metagenomes. However, genes for fatty acid desaturase (FAD) and trehalose synthase (TS) were common in the soil and lake sediment metagenomes. Interestingly, the Lake Untersee water and sediment metagenome samples contained histone-like nucleoid structuring protein (H-NS) and all genes for CIPs. As for the CASPs, high abundances of a wide range of genes for cryo- and osmo-protectants (glutamate, glycine, choline, and betaine) were identified in all metagenomes. However, genes for exopolysaccharide biosynthesis were dominant in Lake Untersee water, sediment, and other soil metagenomes. The results from this study indicate that although diverse microbial communities are present in various metagenomes, they share common cold-responsive stress genes necessary for their survival and sustenance in the extreme Antarctic conditions. |
format |
Text |
author |
Hyunmin Koo Joseph Hakim Casey Morrow Michael Crowley Dale Andersen Asim Bej |
author_facet |
Hyunmin Koo Joseph Hakim Casey Morrow Michael Crowley Dale Andersen Asim Bej |
author_sort |
Hyunmin Koo |
title |
Metagenomic Analysis of Microbial Community Compositions and Cold-Responsive Stress Genes in Selected Antarctic Lacustrine and Soil Ecosystems |
title_short |
Metagenomic Analysis of Microbial Community Compositions and Cold-Responsive Stress Genes in Selected Antarctic Lacustrine and Soil Ecosystems |
title_full |
Metagenomic Analysis of Microbial Community Compositions and Cold-Responsive Stress Genes in Selected Antarctic Lacustrine and Soil Ecosystems |
title_fullStr |
Metagenomic Analysis of Microbial Community Compositions and Cold-Responsive Stress Genes in Selected Antarctic Lacustrine and Soil Ecosystems |
title_full_unstemmed |
Metagenomic Analysis of Microbial Community Compositions and Cold-Responsive Stress Genes in Selected Antarctic Lacustrine and Soil Ecosystems |
title_sort |
metagenomic analysis of microbial community compositions and cold-responsive stress genes in selected antarctic lacustrine and soil ecosystems |
publisher |
Multidisciplinary Digital Publishing Institute |
publishDate |
2018 |
url |
https://doi.org/10.3390/life8030029 |
op_coverage |
agris |
long_lat |
ENVELOPE(78.188,78.188,-68.472,-68.472) ENVELOPE(-100.964,-100.964,56.759,56.759) ENVELOPE(110.533,110.533,-66.267,-66.267) ENVELOPE(13.467,13.467,-71.350,-71.350) |
geographic |
Ace Lake Antarctic Bay Lake McMurdo Dry Valleys Newcomb Bay Untersee |
geographic_facet |
Ace Lake Antarctic Bay Lake McMurdo Dry Valleys Newcomb Bay Untersee |
genre |
Antarc* Antarctic McMurdo Dry Valleys |
genre_facet |
Antarc* Antarctic McMurdo Dry Valleys |
op_source |
Life; Volume 8; Issue 3; Pages: 29 |
op_relation |
Microbiology https://dx.doi.org/10.3390/life8030029 |
op_rights |
https://creativecommons.org/licenses/by/4.0/ |
op_doi |
https://doi.org/10.3390/life8030029 |
container_title |
Life |
container_volume |
8 |
container_issue |
3 |
container_start_page |
29 |
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1774712385507950592 |