HPLC-DAD Purification and Characterization of Meta-Cresol-Purple for Spectrophotometric Seawater pH Measurements
High-quality pH measurements are essential for observing ocean acidification and interpreting its chemical and ecological effects. Spectrophotometric measure is the preferred method for pH analysis of seawater because of its high reproducibility and excellent precision. Meta-cresol purple (mCP) is o...
Published in: | Water |
---|---|
Main Authors: | , , , |
Format: | Text |
Language: | English |
Published: |
Multidisciplinary Digital Publishing Institute
2021
|
Subjects: | |
Online Access: | https://doi.org/10.3390/w13213030 |
id |
ftmdpi:oai:mdpi.com:/2073-4441/13/21/3030/ |
---|---|
record_format |
openpolar |
spelling |
ftmdpi:oai:mdpi.com:/2073-4441/13/21/3030/ 2023-08-20T04:00:43+02:00 HPLC-DAD Purification and Characterization of Meta-Cresol-Purple for Spectrophotometric Seawater pH Measurements Paola Rivaro Davide Vivado Pierpaolo Falco Carmela Ianni agris 2021-10-28 application/pdf https://doi.org/10.3390/w13213030 EN eng Multidisciplinary Digital Publishing Institute Oceans and Coastal Zones https://dx.doi.org/10.3390/w13213030 https://creativecommons.org/licenses/by/4.0/ Water; Volume 13; Issue 21; Pages: 3030 pH meta-cresol purple HPLC ocean acidification Ross Sea Text 2021 ftmdpi https://doi.org/10.3390/w13213030 2023-08-01T03:05:49Z High-quality pH measurements are essential for observing ocean acidification and interpreting its chemical and ecological effects. Spectrophotometric measure is the preferred method for pH analysis of seawater because of its high reproducibility and excellent precision. Meta-cresol purple (mCP) is one of the most used indicator dyes for spectrophotometric pH determination. Impurities in indicator salts can significantly bias spectrophotometric pH determinations, and therefore the use of purified mCP is recommended. This work describes the use of a fast, simple, and green-friendly High-Performance-Liquid Chromatography (HPLC) procedure for purification of mCP dye solution. A C18 reversed phase column was used, and different compositions of the mobile phase were considered. The selected mobile phase was 85% Milli-Q water and 15% CH3CN pH 8.0 that allowed reasonable run time and a suitable purified mCP retention time (4.65 ± 0.005 min). Aliquots of purified mCP were mixed and evaporated by rotary evaporator that resulted in the fastest preconcentration procedure among those considered in this study. Purified mCP was used for measuring the pH in seawater samples collected in the Ross Sea (Antarctica) and the values ranged between 8.202 and 7.983, with the highest values at the surface. Text Antarc* Antarctica Ocean acidification Ross Sea MDPI Open Access Publishing Ross Sea Water 13 21 3030 |
institution |
Open Polar |
collection |
MDPI Open Access Publishing |
op_collection_id |
ftmdpi |
language |
English |
topic |
pH meta-cresol purple HPLC ocean acidification Ross Sea |
spellingShingle |
pH meta-cresol purple HPLC ocean acidification Ross Sea Paola Rivaro Davide Vivado Pierpaolo Falco Carmela Ianni HPLC-DAD Purification and Characterization of Meta-Cresol-Purple for Spectrophotometric Seawater pH Measurements |
topic_facet |
pH meta-cresol purple HPLC ocean acidification Ross Sea |
description |
High-quality pH measurements are essential for observing ocean acidification and interpreting its chemical and ecological effects. Spectrophotometric measure is the preferred method for pH analysis of seawater because of its high reproducibility and excellent precision. Meta-cresol purple (mCP) is one of the most used indicator dyes for spectrophotometric pH determination. Impurities in indicator salts can significantly bias spectrophotometric pH determinations, and therefore the use of purified mCP is recommended. This work describes the use of a fast, simple, and green-friendly High-Performance-Liquid Chromatography (HPLC) procedure for purification of mCP dye solution. A C18 reversed phase column was used, and different compositions of the mobile phase were considered. The selected mobile phase was 85% Milli-Q water and 15% CH3CN pH 8.0 that allowed reasonable run time and a suitable purified mCP retention time (4.65 ± 0.005 min). Aliquots of purified mCP were mixed and evaporated by rotary evaporator that resulted in the fastest preconcentration procedure among those considered in this study. Purified mCP was used for measuring the pH in seawater samples collected in the Ross Sea (Antarctica) and the values ranged between 8.202 and 7.983, with the highest values at the surface. |
format |
Text |
author |
Paola Rivaro Davide Vivado Pierpaolo Falco Carmela Ianni |
author_facet |
Paola Rivaro Davide Vivado Pierpaolo Falco Carmela Ianni |
author_sort |
Paola Rivaro |
title |
HPLC-DAD Purification and Characterization of Meta-Cresol-Purple for Spectrophotometric Seawater pH Measurements |
title_short |
HPLC-DAD Purification and Characterization of Meta-Cresol-Purple for Spectrophotometric Seawater pH Measurements |
title_full |
HPLC-DAD Purification and Characterization of Meta-Cresol-Purple for Spectrophotometric Seawater pH Measurements |
title_fullStr |
HPLC-DAD Purification and Characterization of Meta-Cresol-Purple for Spectrophotometric Seawater pH Measurements |
title_full_unstemmed |
HPLC-DAD Purification and Characterization of Meta-Cresol-Purple for Spectrophotometric Seawater pH Measurements |
title_sort |
hplc-dad purification and characterization of meta-cresol-purple for spectrophotometric seawater ph measurements |
publisher |
Multidisciplinary Digital Publishing Institute |
publishDate |
2021 |
url |
https://doi.org/10.3390/w13213030 |
op_coverage |
agris |
geographic |
Ross Sea |
geographic_facet |
Ross Sea |
genre |
Antarc* Antarctica Ocean acidification Ross Sea |
genre_facet |
Antarc* Antarctica Ocean acidification Ross Sea |
op_source |
Water; Volume 13; Issue 21; Pages: 3030 |
op_relation |
Oceans and Coastal Zones https://dx.doi.org/10.3390/w13213030 |
op_rights |
https://creativecommons.org/licenses/by/4.0/ |
op_doi |
https://doi.org/10.3390/w13213030 |
container_title |
Water |
container_volume |
13 |
container_issue |
21 |
container_start_page |
3030 |
_version_ |
1774719873589444608 |