Comparative Genomic Analysis of Antarctic Pseudomonas Isolates with 2,4,6-Trinitrotoluene Transformation Capabilities Reveals Their Unique Features for Xenobiotics Degradation
The nitroaromatic explosive 2,4,6-trinitrotoluene (TNT) is a highly toxic and persistent environmental pollutant. Since physicochemical methods for remediation are poorly effective, the use of microorganisms has gained interest as an alternative to restore TNT-contaminated sites. We previously demon...
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ftmdpi:oai:mdpi.com:/2073-4425/13/8/1354/ 2023-08-20T04:02:28+02:00 Comparative Genomic Analysis of Antarctic Pseudomonas Isolates with 2,4,6-Trinitrotoluene Transformation Capabilities Reveals Their Unique Features for Xenobiotics Degradation Ma. Ángeles Cabrera Sebastián L. Márquez José M. Pérez-Donoso agris 2022-07-28 application/pdf https://doi.org/10.3390/genes13081354 EN eng Multidisciplinary Digital Publishing Institute Microbial Genetics and Genomics https://dx.doi.org/10.3390/genes13081354 https://creativecommons.org/licenses/by/4.0/ Genes; Volume 13; Issue 8; Pages: 1354 Pseudomonas TNT comparative genomics Antarctica bioremediation xenobiotics Text 2022 ftmdpi https://doi.org/10.3390/genes13081354 2023-08-01T05:52:46Z The nitroaromatic explosive 2,4,6-trinitrotoluene (TNT) is a highly toxic and persistent environmental pollutant. Since physicochemical methods for remediation are poorly effective, the use of microorganisms has gained interest as an alternative to restore TNT-contaminated sites. We previously demonstrated the high TNT-transforming capability of three novel Pseudomonas spp. isolated from Deception Island, Antarctica, which exceeded that of the well-characterized TNT-degrading bacterium Pseudomonas putida KT2440. In this study, a comparative genomic analysis was performed to search for the metabolic functions encoded in the genomes of these isolates that might explain their TNT-transforming phenotype, and also to look for differences with 21 other selected pseudomonads, including xenobiotics-degrading species. Comparative analysis of xenobiotic degradation pathways revealed that our isolates have the highest abundance of key enzymes related to the degradation of fluorobenzoate, TNT, and bisphenol A. Further comparisons considering only TNT-transforming pseudomonads revealed the presence of unique genes in these isolates that would likely participate directly in TNT-transformation, and others involved in the β-ketoadipate pathway for aromatic compound degradation. Lastly, the phylogenomic analysis suggested that these Antarctic isolates likely represent novel species of the genus Pseudomonas, which emphasizes their relevance as potential agents for the bioremediation of TNT and other xenobiotics. Text Antarc* Antarctic Antarctica Deception Island MDPI Open Access Publishing Antarctic Deception Island ENVELOPE(-60.633,-60.633,-62.950,-62.950) Genes 13 8 1354 |
institution |
Open Polar |
collection |
MDPI Open Access Publishing |
op_collection_id |
ftmdpi |
language |
English |
topic |
Pseudomonas TNT comparative genomics Antarctica bioremediation xenobiotics |
spellingShingle |
Pseudomonas TNT comparative genomics Antarctica bioremediation xenobiotics Ma. Ángeles Cabrera Sebastián L. Márquez José M. Pérez-Donoso Comparative Genomic Analysis of Antarctic Pseudomonas Isolates with 2,4,6-Trinitrotoluene Transformation Capabilities Reveals Their Unique Features for Xenobiotics Degradation |
topic_facet |
Pseudomonas TNT comparative genomics Antarctica bioremediation xenobiotics |
description |
The nitroaromatic explosive 2,4,6-trinitrotoluene (TNT) is a highly toxic and persistent environmental pollutant. Since physicochemical methods for remediation are poorly effective, the use of microorganisms has gained interest as an alternative to restore TNT-contaminated sites. We previously demonstrated the high TNT-transforming capability of three novel Pseudomonas spp. isolated from Deception Island, Antarctica, which exceeded that of the well-characterized TNT-degrading bacterium Pseudomonas putida KT2440. In this study, a comparative genomic analysis was performed to search for the metabolic functions encoded in the genomes of these isolates that might explain their TNT-transforming phenotype, and also to look for differences with 21 other selected pseudomonads, including xenobiotics-degrading species. Comparative analysis of xenobiotic degradation pathways revealed that our isolates have the highest abundance of key enzymes related to the degradation of fluorobenzoate, TNT, and bisphenol A. Further comparisons considering only TNT-transforming pseudomonads revealed the presence of unique genes in these isolates that would likely participate directly in TNT-transformation, and others involved in the β-ketoadipate pathway for aromatic compound degradation. Lastly, the phylogenomic analysis suggested that these Antarctic isolates likely represent novel species of the genus Pseudomonas, which emphasizes their relevance as potential agents for the bioremediation of TNT and other xenobiotics. |
format |
Text |
author |
Ma. Ángeles Cabrera Sebastián L. Márquez José M. Pérez-Donoso |
author_facet |
Ma. Ángeles Cabrera Sebastián L. Márquez José M. Pérez-Donoso |
author_sort |
Ma. Ángeles Cabrera |
title |
Comparative Genomic Analysis of Antarctic Pseudomonas Isolates with 2,4,6-Trinitrotoluene Transformation Capabilities Reveals Their Unique Features for Xenobiotics Degradation |
title_short |
Comparative Genomic Analysis of Antarctic Pseudomonas Isolates with 2,4,6-Trinitrotoluene Transformation Capabilities Reveals Their Unique Features for Xenobiotics Degradation |
title_full |
Comparative Genomic Analysis of Antarctic Pseudomonas Isolates with 2,4,6-Trinitrotoluene Transformation Capabilities Reveals Their Unique Features for Xenobiotics Degradation |
title_fullStr |
Comparative Genomic Analysis of Antarctic Pseudomonas Isolates with 2,4,6-Trinitrotoluene Transformation Capabilities Reveals Their Unique Features for Xenobiotics Degradation |
title_full_unstemmed |
Comparative Genomic Analysis of Antarctic Pseudomonas Isolates with 2,4,6-Trinitrotoluene Transformation Capabilities Reveals Their Unique Features for Xenobiotics Degradation |
title_sort |
comparative genomic analysis of antarctic pseudomonas isolates with 2,4,6-trinitrotoluene transformation capabilities reveals their unique features for xenobiotics degradation |
publisher |
Multidisciplinary Digital Publishing Institute |
publishDate |
2022 |
url |
https://doi.org/10.3390/genes13081354 |
op_coverage |
agris |
long_lat |
ENVELOPE(-60.633,-60.633,-62.950,-62.950) |
geographic |
Antarctic Deception Island |
geographic_facet |
Antarctic Deception Island |
genre |
Antarc* Antarctic Antarctica Deception Island |
genre_facet |
Antarc* Antarctic Antarctica Deception Island |
op_source |
Genes; Volume 13; Issue 8; Pages: 1354 |
op_relation |
Microbial Genetics and Genomics https://dx.doi.org/10.3390/genes13081354 |
op_rights |
https://creativecommons.org/licenses/by/4.0/ |
op_doi |
https://doi.org/10.3390/genes13081354 |
container_title |
Genes |
container_volume |
13 |
container_issue |
8 |
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1354 |
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1774712918506471424 |