Molecular Responses to Thermal and Osmotic Stress in Arctic Intertidal Mussels (Mytilus edulis): The Limits of Resilience
Increases in Arctic temperatures have accelerated melting of the Greenland icesheet, exposing intertidal organisms, such as the blue mussel Mytilus edulis, to high air temperatures and low salinities in summer. However, the interaction of these combined stressors is poorly described at the transcrip...
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ftmdpi:oai:mdpi.com:/2073-4425/13/1/155/ 2023-08-20T04:04:20+02:00 Molecular Responses to Thermal and Osmotic Stress in Arctic Intertidal Mussels (Mytilus edulis): The Limits of Resilience Nicholas J. Barrett Jakob Thyrring Elizabeth M. Harper Mikael K. Sejr Jesper G. Sørensen Lloyd S. Peck Melody S. Clark agris 2022-01-15 application/pdf https://doi.org/10.3390/genes13010155 EN eng Multidisciplinary Digital Publishing Institute Population and Evolutionary Genetics and Genomics https://dx.doi.org/10.3390/genes13010155 https://creativecommons.org/licenses/by/4.0/ Genes; Volume 13; Issue 1; Pages: 155 blue mussel cellular stress response salinity thermal tolerance transcriptome acclimation freshening climate change aquaporins Text 2022 ftmdpi https://doi.org/10.3390/genes13010155 2023-08-01T03:51:04Z Increases in Arctic temperatures have accelerated melting of the Greenland icesheet, exposing intertidal organisms, such as the blue mussel Mytilus edulis, to high air temperatures and low salinities in summer. However, the interaction of these combined stressors is poorly described at the transcriptional level. Comparing expression profiles of M. edulis from experimentally warmed (30 °C and 33 °C) animals kept at control (23‰) and low salinities (15‰) revealed a significant lack of enrichment for Gene Ontology terms (GO), indicating that similar processes were active under all conditions. However, there was a progressive increase in the abundance of upregulated genes as each stressor was applied, with synergistic increases at 33 °C and 15‰, suggesting combined stressors push the animal towards their tolerance thresholds. Further analyses comparing the effects of salinity alone (23‰, 15‰ and 5‰) showed high expression of stress and osmoregulatory marker genes at the lowest salinity, implying that the cell is carrying out intracellular osmoregulation to maintain the cytosol as hyperosmotic. Identification of aquaporins and vacuolar-type ATPase transcripts suggested the cell may use fluid-filled cavities to excrete excess intracellular water, as previously identified in embryonic freshwater mussels. These results indicate that M. edulis has considerable resilience to heat stress and highly efficient mechanisms to acclimatise to lowered salinity in a changing world. Text Arctic Climate change Greenland MDPI Open Access Publishing Arctic Greenland Genes 13 1 155 |
institution |
Open Polar |
collection |
MDPI Open Access Publishing |
op_collection_id |
ftmdpi |
language |
English |
topic |
blue mussel cellular stress response salinity thermal tolerance transcriptome acclimation freshening climate change aquaporins |
spellingShingle |
blue mussel cellular stress response salinity thermal tolerance transcriptome acclimation freshening climate change aquaporins Nicholas J. Barrett Jakob Thyrring Elizabeth M. Harper Mikael K. Sejr Jesper G. Sørensen Lloyd S. Peck Melody S. Clark Molecular Responses to Thermal and Osmotic Stress in Arctic Intertidal Mussels (Mytilus edulis): The Limits of Resilience |
topic_facet |
blue mussel cellular stress response salinity thermal tolerance transcriptome acclimation freshening climate change aquaporins |
description |
Increases in Arctic temperatures have accelerated melting of the Greenland icesheet, exposing intertidal organisms, such as the blue mussel Mytilus edulis, to high air temperatures and low salinities in summer. However, the interaction of these combined stressors is poorly described at the transcriptional level. Comparing expression profiles of M. edulis from experimentally warmed (30 °C and 33 °C) animals kept at control (23‰) and low salinities (15‰) revealed a significant lack of enrichment for Gene Ontology terms (GO), indicating that similar processes were active under all conditions. However, there was a progressive increase in the abundance of upregulated genes as each stressor was applied, with synergistic increases at 33 °C and 15‰, suggesting combined stressors push the animal towards their tolerance thresholds. Further analyses comparing the effects of salinity alone (23‰, 15‰ and 5‰) showed high expression of stress and osmoregulatory marker genes at the lowest salinity, implying that the cell is carrying out intracellular osmoregulation to maintain the cytosol as hyperosmotic. Identification of aquaporins and vacuolar-type ATPase transcripts suggested the cell may use fluid-filled cavities to excrete excess intracellular water, as previously identified in embryonic freshwater mussels. These results indicate that M. edulis has considerable resilience to heat stress and highly efficient mechanisms to acclimatise to lowered salinity in a changing world. |
format |
Text |
author |
Nicholas J. Barrett Jakob Thyrring Elizabeth M. Harper Mikael K. Sejr Jesper G. Sørensen Lloyd S. Peck Melody S. Clark |
author_facet |
Nicholas J. Barrett Jakob Thyrring Elizabeth M. Harper Mikael K. Sejr Jesper G. Sørensen Lloyd S. Peck Melody S. Clark |
author_sort |
Nicholas J. Barrett |
title |
Molecular Responses to Thermal and Osmotic Stress in Arctic Intertidal Mussels (Mytilus edulis): The Limits of Resilience |
title_short |
Molecular Responses to Thermal and Osmotic Stress in Arctic Intertidal Mussels (Mytilus edulis): The Limits of Resilience |
title_full |
Molecular Responses to Thermal and Osmotic Stress in Arctic Intertidal Mussels (Mytilus edulis): The Limits of Resilience |
title_fullStr |
Molecular Responses to Thermal and Osmotic Stress in Arctic Intertidal Mussels (Mytilus edulis): The Limits of Resilience |
title_full_unstemmed |
Molecular Responses to Thermal and Osmotic Stress in Arctic Intertidal Mussels (Mytilus edulis): The Limits of Resilience |
title_sort |
molecular responses to thermal and osmotic stress in arctic intertidal mussels (mytilus edulis): the limits of resilience |
publisher |
Multidisciplinary Digital Publishing Institute |
publishDate |
2022 |
url |
https://doi.org/10.3390/genes13010155 |
op_coverage |
agris |
geographic |
Arctic Greenland |
geographic_facet |
Arctic Greenland |
genre |
Arctic Climate change Greenland |
genre_facet |
Arctic Climate change Greenland |
op_source |
Genes; Volume 13; Issue 1; Pages: 155 |
op_relation |
Population and Evolutionary Genetics and Genomics https://dx.doi.org/10.3390/genes13010155 |
op_rights |
https://creativecommons.org/licenses/by/4.0/ |
op_doi |
https://doi.org/10.3390/genes13010155 |
container_title |
Genes |
container_volume |
13 |
container_issue |
1 |
container_start_page |
155 |
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1774714722439921664 |