Genomics Insights into Pseudomonas sp. CG01: An Antarctic Cadmium-Resistant Strain Capable of Biosynthesizing CdS Nanoparticles Using Methionine as S-Source
Here, we present the draft genome sequence of Pseudomonas sp. GC01, a cadmium-resistant Antarctic bacterium capable of biosynthesizing CdS fluorescent nanoparticles (quantum dots, QDs) employing a unique mechanism involving the production of methanethiol (MeSH) from methionine (Met). To explore the...
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ftmdpi:oai:mdpi.com:/2073-4425/12/2/187/ 2023-08-20T04:02:22+02:00 Genomics Insights into Pseudomonas sp. CG01: An Antarctic Cadmium-Resistant Strain Capable of Biosynthesizing CdS Nanoparticles Using Methionine as S-Source Carla Gallardo-Benavente Jessica L. Campo-Giraldo Juan Castro-Severyn Andrés Quiroz José M. Pérez-Donoso agris 2021-01-27 application/pdf https://doi.org/10.3390/genes12020187 EN eng Multidisciplinary Digital Publishing Institute Microbial Genetics and Genomics https://dx.doi.org/10.3390/genes12020187 https://creativecommons.org/licenses/by/4.0/ Genes; Volume 12; Issue 2; Pages: 187 Antarctic bacteria nanoparticle biosynthesis comparative genomics volatile sulfur compounds Text 2021 ftmdpi https://doi.org/10.3390/genes12020187 2023-08-01T00:57:08Z Here, we present the draft genome sequence of Pseudomonas sp. GC01, a cadmium-resistant Antarctic bacterium capable of biosynthesizing CdS fluorescent nanoparticles (quantum dots, QDs) employing a unique mechanism involving the production of methanethiol (MeSH) from methionine (Met). To explore the molecular/metabolic components involved in QDs biosynthesis, we conducted a comparative genomic analysis, searching for the genes related to cadmium resistance and sulfur metabolic pathways. The genome of Pseudomonas sp. GC01 has a 4,706,645 bp size with a 58.61% G+C content. Pseudomonas sp. GC01 possesses five genes related to cadmium transport/resistance, with three P-type ATPases (cadA, zntA, and pbrA) involved in Cd-secretion that could contribute to the extracellular biosynthesis of CdS QDs. Furthermore, it exhibits genes involved in sulfate assimilation, cysteine/methionine synthesis, and volatile sulfur compounds catabolic pathways. Regarding MeSH production from Met, Pseudomonas sp. GC01 lacks the genes E4.4.1.11 and megL for MeSH generation. Interestingly, despite the absence of these genes, Pseudomonas sp. GC01 produces high levels of MeSH. This is probably associated with the metC gene that also produces MeSH from Met in bacteria. This work is the first report of the potential genes involved in Cd resistance, sulfur metabolism, and the process of MeSH-dependent CdS QDs bioproduction in Pseudomonas spp. strains. Text Antarc* Antarctic MDPI Open Access Publishing Antarctic Genes 12 2 187 |
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Open Polar |
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MDPI Open Access Publishing |
op_collection_id |
ftmdpi |
language |
English |
topic |
Antarctic bacteria nanoparticle biosynthesis comparative genomics volatile sulfur compounds |
spellingShingle |
Antarctic bacteria nanoparticle biosynthesis comparative genomics volatile sulfur compounds Carla Gallardo-Benavente Jessica L. Campo-Giraldo Juan Castro-Severyn Andrés Quiroz José M. Pérez-Donoso Genomics Insights into Pseudomonas sp. CG01: An Antarctic Cadmium-Resistant Strain Capable of Biosynthesizing CdS Nanoparticles Using Methionine as S-Source |
topic_facet |
Antarctic bacteria nanoparticle biosynthesis comparative genomics volatile sulfur compounds |
description |
Here, we present the draft genome sequence of Pseudomonas sp. GC01, a cadmium-resistant Antarctic bacterium capable of biosynthesizing CdS fluorescent nanoparticles (quantum dots, QDs) employing a unique mechanism involving the production of methanethiol (MeSH) from methionine (Met). To explore the molecular/metabolic components involved in QDs biosynthesis, we conducted a comparative genomic analysis, searching for the genes related to cadmium resistance and sulfur metabolic pathways. The genome of Pseudomonas sp. GC01 has a 4,706,645 bp size with a 58.61% G+C content. Pseudomonas sp. GC01 possesses five genes related to cadmium transport/resistance, with three P-type ATPases (cadA, zntA, and pbrA) involved in Cd-secretion that could contribute to the extracellular biosynthesis of CdS QDs. Furthermore, it exhibits genes involved in sulfate assimilation, cysteine/methionine synthesis, and volatile sulfur compounds catabolic pathways. Regarding MeSH production from Met, Pseudomonas sp. GC01 lacks the genes E4.4.1.11 and megL for MeSH generation. Interestingly, despite the absence of these genes, Pseudomonas sp. GC01 produces high levels of MeSH. This is probably associated with the metC gene that also produces MeSH from Met in bacteria. This work is the first report of the potential genes involved in Cd resistance, sulfur metabolism, and the process of MeSH-dependent CdS QDs bioproduction in Pseudomonas spp. strains. |
format |
Text |
author |
Carla Gallardo-Benavente Jessica L. Campo-Giraldo Juan Castro-Severyn Andrés Quiroz José M. Pérez-Donoso |
author_facet |
Carla Gallardo-Benavente Jessica L. Campo-Giraldo Juan Castro-Severyn Andrés Quiroz José M. Pérez-Donoso |
author_sort |
Carla Gallardo-Benavente |
title |
Genomics Insights into Pseudomonas sp. CG01: An Antarctic Cadmium-Resistant Strain Capable of Biosynthesizing CdS Nanoparticles Using Methionine as S-Source |
title_short |
Genomics Insights into Pseudomonas sp. CG01: An Antarctic Cadmium-Resistant Strain Capable of Biosynthesizing CdS Nanoparticles Using Methionine as S-Source |
title_full |
Genomics Insights into Pseudomonas sp. CG01: An Antarctic Cadmium-Resistant Strain Capable of Biosynthesizing CdS Nanoparticles Using Methionine as S-Source |
title_fullStr |
Genomics Insights into Pseudomonas sp. CG01: An Antarctic Cadmium-Resistant Strain Capable of Biosynthesizing CdS Nanoparticles Using Methionine as S-Source |
title_full_unstemmed |
Genomics Insights into Pseudomonas sp. CG01: An Antarctic Cadmium-Resistant Strain Capable of Biosynthesizing CdS Nanoparticles Using Methionine as S-Source |
title_sort |
genomics insights into pseudomonas sp. cg01: an antarctic cadmium-resistant strain capable of biosynthesizing cds nanoparticles using methionine as s-source |
publisher |
Multidisciplinary Digital Publishing Institute |
publishDate |
2021 |
url |
https://doi.org/10.3390/genes12020187 |
op_coverage |
agris |
geographic |
Antarctic |
geographic_facet |
Antarctic |
genre |
Antarc* Antarctic |
genre_facet |
Antarc* Antarctic |
op_source |
Genes; Volume 12; Issue 2; Pages: 187 |
op_relation |
Microbial Genetics and Genomics https://dx.doi.org/10.3390/genes12020187 |
op_rights |
https://creativecommons.org/licenses/by/4.0/ |
op_doi |
https://doi.org/10.3390/genes12020187 |
container_title |
Genes |
container_volume |
12 |
container_issue |
2 |
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187 |
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1774712797599367168 |