Transcriptional Differences between Canine Cutaneous Epitheliotropic Lymphoma and Immune-Mediated Dermatoses
Canine cutaneous epitheliotropic T-cell lymphoma (CETL) and immune-mediated T-cell predominant dermatoses (IMD) share several clinical and histopathological features, but differ substantially in prognosis. The discrimination of ambiguous cases may be challenging, as diagnostic tests are limited and...
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ftmdpi:oai:mdpi.com:/2073-4425/12/2/160/ 2023-08-20T04:05:48+02:00 Transcriptional Differences between Canine Cutaneous Epitheliotropic Lymphoma and Immune-Mediated Dermatoses Nadja Gerber Magdalena A. T. Brunner Vidhya Jagannathan Tosso Leeb Nora M. Gerhards Monika M. Welle Martina Dettwiler agris 2021-01-25 application/pdf https://doi.org/10.3390/genes12020160 EN eng Multidisciplinary Digital Publishing Institute Molecular Genetics and Genomics https://dx.doi.org/10.3390/genes12020160 https://creativecommons.org/licenses/by/4.0/ Genes; Volume 12; Issue 2; Pages: 160 Canis lupus familiaris dog skin disease cutaneous T-cell lymphoma lupus erythematosus cytotoxic dermatitis RNA sequencing FFPE tissue transcriptome Text 2021 ftmdpi https://doi.org/10.3390/genes12020160 2023-08-01T00:56:06Z Canine cutaneous epitheliotropic T-cell lymphoma (CETL) and immune-mediated T-cell predominant dermatoses (IMD) share several clinical and histopathological features, but differ substantially in prognosis. The discrimination of ambiguous cases may be challenging, as diagnostic tests are limited and may prove equivocal. This study aimed to investigate transcriptional differences between CETL and IMD, as a basis for further research on discriminating diagnostic biomarkers. We performed 100bp single-end sequencing on RNA extracted from formalin-fixed and paraffin-embedded skin biopsies from dogs with CETL and IMD, respectively. DESeq2 was used for principal component analysis (PCA) and differential gene expression analysis. Genes with significantly different expression were analyzed for enriched pathways using two different tools. The expression of selected genes and their proteins was validated by RT-qPCR and immunohistochemistry. PCA demonstrated the distinct gene expression profiles of CETL and IMD. In total, 503 genes were upregulated, while 4986 were downregulated in CETL compared to IMD. RT-qPCR confirmed the sequencing results for 5/6 selected genes tested, while the protein expression detected by immunohistochemistry was not entirely consistent. Our study revealed transcriptional differences between canine CETL and IMD, with similarities to human cutaneous lymphoma. Differentially expressed genes are potential discriminatory markers, but require further validation on larger sample collections. Text Canis lupus MDPI Open Access Publishing Genes 12 2 160 |
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Open Polar |
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MDPI Open Access Publishing |
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ftmdpi |
language |
English |
topic |
Canis lupus familiaris dog skin disease cutaneous T-cell lymphoma lupus erythematosus cytotoxic dermatitis RNA sequencing FFPE tissue transcriptome |
spellingShingle |
Canis lupus familiaris dog skin disease cutaneous T-cell lymphoma lupus erythematosus cytotoxic dermatitis RNA sequencing FFPE tissue transcriptome Nadja Gerber Magdalena A. T. Brunner Vidhya Jagannathan Tosso Leeb Nora M. Gerhards Monika M. Welle Martina Dettwiler Transcriptional Differences between Canine Cutaneous Epitheliotropic Lymphoma and Immune-Mediated Dermatoses |
topic_facet |
Canis lupus familiaris dog skin disease cutaneous T-cell lymphoma lupus erythematosus cytotoxic dermatitis RNA sequencing FFPE tissue transcriptome |
description |
Canine cutaneous epitheliotropic T-cell lymphoma (CETL) and immune-mediated T-cell predominant dermatoses (IMD) share several clinical and histopathological features, but differ substantially in prognosis. The discrimination of ambiguous cases may be challenging, as diagnostic tests are limited and may prove equivocal. This study aimed to investigate transcriptional differences between CETL and IMD, as a basis for further research on discriminating diagnostic biomarkers. We performed 100bp single-end sequencing on RNA extracted from formalin-fixed and paraffin-embedded skin biopsies from dogs with CETL and IMD, respectively. DESeq2 was used for principal component analysis (PCA) and differential gene expression analysis. Genes with significantly different expression were analyzed for enriched pathways using two different tools. The expression of selected genes and their proteins was validated by RT-qPCR and immunohistochemistry. PCA demonstrated the distinct gene expression profiles of CETL and IMD. In total, 503 genes were upregulated, while 4986 were downregulated in CETL compared to IMD. RT-qPCR confirmed the sequencing results for 5/6 selected genes tested, while the protein expression detected by immunohistochemistry was not entirely consistent. Our study revealed transcriptional differences between canine CETL and IMD, with similarities to human cutaneous lymphoma. Differentially expressed genes are potential discriminatory markers, but require further validation on larger sample collections. |
format |
Text |
author |
Nadja Gerber Magdalena A. T. Brunner Vidhya Jagannathan Tosso Leeb Nora M. Gerhards Monika M. Welle Martina Dettwiler |
author_facet |
Nadja Gerber Magdalena A. T. Brunner Vidhya Jagannathan Tosso Leeb Nora M. Gerhards Monika M. Welle Martina Dettwiler |
author_sort |
Nadja Gerber |
title |
Transcriptional Differences between Canine Cutaneous Epitheliotropic Lymphoma and Immune-Mediated Dermatoses |
title_short |
Transcriptional Differences between Canine Cutaneous Epitheliotropic Lymphoma and Immune-Mediated Dermatoses |
title_full |
Transcriptional Differences between Canine Cutaneous Epitheliotropic Lymphoma and Immune-Mediated Dermatoses |
title_fullStr |
Transcriptional Differences between Canine Cutaneous Epitheliotropic Lymphoma and Immune-Mediated Dermatoses |
title_full_unstemmed |
Transcriptional Differences between Canine Cutaneous Epitheliotropic Lymphoma and Immune-Mediated Dermatoses |
title_sort |
transcriptional differences between canine cutaneous epitheliotropic lymphoma and immune-mediated dermatoses |
publisher |
Multidisciplinary Digital Publishing Institute |
publishDate |
2021 |
url |
https://doi.org/10.3390/genes12020160 |
op_coverage |
agris |
genre |
Canis lupus |
genre_facet |
Canis lupus |
op_source |
Genes; Volume 12; Issue 2; Pages: 160 |
op_relation |
Molecular Genetics and Genomics https://dx.doi.org/10.3390/genes12020160 |
op_rights |
https://creativecommons.org/licenses/by/4.0/ |
op_doi |
https://doi.org/10.3390/genes12020160 |
container_title |
Genes |
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12 |
container_issue |
2 |
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160 |
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1774716541418340352 |